Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract
Objectives: β-Escin, one of the constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), inhibits NF-κB activation, which plays an important role in HSV-1 replication. The aim was to examine the antiherpetic activity of β-escin and AH, as well as their effect on the activation...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223573_v70_n11_p1561_Michelini http://hdl.handle.net/20.500.12110/paper_00223573_v70_n11_p1561_Michelini |
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paper:paper_00223573_v70_n11_p1561_Michelini2023-06-08T14:50:02Z Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract Aesculus hippocastanum antiviral immunomodulatory medicinal plants β-escin Aesculus hippocastanum extract beta escin immunoglobulin enhancer binding protein toll like receptor transcription factor AP 1 antivirus agent autacoid cytokine escin immunoglobulin enhancer binding protein immunologic factor plant extract transcription factor AP 1 animal cell antiviral activity Article cell viability controlled study cytokine production Dengue virus epithelium cell herpes simplex human Human alphaherpesvirus 1 human cell immunomodulation inflammation macrophage nonhuman Vesiculovirus A-549 cell line Aesculus animal chemistry drug effect immunology isolation and purification metabolism mouse pathogenicity virology virus A549 Cells Aesculus Animals Antiviral Agents Cytokines Dengue Virus Epithelial Cells Escin Herpesvirus 1, Human Humans Immunologic Factors Inflammation Mediators Macrophages Mice NF-kappa B Plant Extracts Transcription Factor AP-1 Vesiculovirus Viruses Objectives: β-Escin, one of the constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), inhibits NF-κB activation, which plays an important role in HSV-1 replication. The aim was to examine the antiherpetic activity of β-escin and AH, as well as their effect on the activation of NF-κB and AP-1 and cytokine secretion in epithelial cells and macrophages. Methods: Cell viability was evaluated using MTT assay, and antiviral and virucidal activity was determined by plaque assay. The effect on NF-κB and AP-1 signalling pathways activation was determined by a luciferase reporter assay, and cytokine production was measured by ELISA. Key findings: β-Escin and AH had virucidal and anti-HSV-1 activities, and the antiviral activity was discovered for other enveloped viruses (VSV and Dengue). Moreover, β-escin and AH significantly reduced NF-κB and AP-1 activation and cytokine production in macrophages stimulated with HSV-1 and TLRs ligands. However, an enhanced activation of these pathways and an increase in the levels of pro-inflammatory cytokines in β-escin and AH-treated HSV-1-infected epithelial cells were found. Conclusions: This study demonstrates virucidal and broad-spectrum antiviral activities for β escin and AH. Besides, β-escin and AH modulate cytokine production depending on the stimuli (viral or non-viral) and the cell type under study. © 2018 Royal Pharmaceutical Society 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223573_v70_n11_p1561_Michelini http://hdl.handle.net/20.500.12110/paper_00223573_v70_n11_p1561_Michelini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aesculus hippocastanum antiviral immunomodulatory medicinal plants β-escin Aesculus hippocastanum extract beta escin immunoglobulin enhancer binding protein toll like receptor transcription factor AP 1 antivirus agent autacoid cytokine escin immunoglobulin enhancer binding protein immunologic factor plant extract transcription factor AP 1 animal cell antiviral activity Article cell viability controlled study cytokine production Dengue virus epithelium cell herpes simplex human Human alphaherpesvirus 1 human cell immunomodulation inflammation macrophage nonhuman Vesiculovirus A-549 cell line Aesculus animal chemistry drug effect immunology isolation and purification metabolism mouse pathogenicity virology virus A549 Cells Aesculus Animals Antiviral Agents Cytokines Dengue Virus Epithelial Cells Escin Herpesvirus 1, Human Humans Immunologic Factors Inflammation Mediators Macrophages Mice NF-kappa B Plant Extracts Transcription Factor AP-1 Vesiculovirus Viruses |
| spellingShingle |
Aesculus hippocastanum antiviral immunomodulatory medicinal plants β-escin Aesculus hippocastanum extract beta escin immunoglobulin enhancer binding protein toll like receptor transcription factor AP 1 antivirus agent autacoid cytokine escin immunoglobulin enhancer binding protein immunologic factor plant extract transcription factor AP 1 animal cell antiviral activity Article cell viability controlled study cytokine production Dengue virus epithelium cell herpes simplex human Human alphaherpesvirus 1 human cell immunomodulation inflammation macrophage nonhuman Vesiculovirus A-549 cell line Aesculus animal chemistry drug effect immunology isolation and purification metabolism mouse pathogenicity virology virus A549 Cells Aesculus Animals Antiviral Agents Cytokines Dengue Virus Epithelial Cells Escin Herpesvirus 1, Human Humans Immunologic Factors Inflammation Mediators Macrophages Mice NF-kappa B Plant Extracts Transcription Factor AP-1 Vesiculovirus Viruses Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract |
| topic_facet |
Aesculus hippocastanum antiviral immunomodulatory medicinal plants β-escin Aesculus hippocastanum extract beta escin immunoglobulin enhancer binding protein toll like receptor transcription factor AP 1 antivirus agent autacoid cytokine escin immunoglobulin enhancer binding protein immunologic factor plant extract transcription factor AP 1 animal cell antiviral activity Article cell viability controlled study cytokine production Dengue virus epithelium cell herpes simplex human Human alphaherpesvirus 1 human cell immunomodulation inflammation macrophage nonhuman Vesiculovirus A-549 cell line Aesculus animal chemistry drug effect immunology isolation and purification metabolism mouse pathogenicity virology virus A549 Cells Aesculus Animals Antiviral Agents Cytokines Dengue Virus Epithelial Cells Escin Herpesvirus 1, Human Humans Immunologic Factors Inflammation Mediators Macrophages Mice NF-kappa B Plant Extracts Transcription Factor AP-1 Vesiculovirus Viruses |
| description |
Objectives: β-Escin, one of the constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), inhibits NF-κB activation, which plays an important role in HSV-1 replication. The aim was to examine the antiherpetic activity of β-escin and AH, as well as their effect on the activation of NF-κB and AP-1 and cytokine secretion in epithelial cells and macrophages. Methods: Cell viability was evaluated using MTT assay, and antiviral and virucidal activity was determined by plaque assay. The effect on NF-κB and AP-1 signalling pathways activation was determined by a luciferase reporter assay, and cytokine production was measured by ELISA. Key findings: β-Escin and AH had virucidal and anti-HSV-1 activities, and the antiviral activity was discovered for other enveloped viruses (VSV and Dengue). Moreover, β-escin and AH significantly reduced NF-κB and AP-1 activation and cytokine production in macrophages stimulated with HSV-1 and TLRs ligands. However, an enhanced activation of these pathways and an increase in the levels of pro-inflammatory cytokines in β-escin and AH-treated HSV-1-infected epithelial cells were found. Conclusions: This study demonstrates virucidal and broad-spectrum antiviral activities for β escin and AH. Besides, β-escin and AH modulate cytokine production depending on the stimuli (viral or non-viral) and the cell type under study. © 2018 Royal Pharmaceutical Society |
| title |
Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract |
| title_short |
Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract |
| title_full |
Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract |
| title_fullStr |
Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract |
| title_full_unstemmed |
Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract |
| title_sort |
virucidal, antiviral and immunomodulatory activities of β-escin and aesculus hippocastanum extract |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223573_v70_n11_p1561_Michelini http://hdl.handle.net/20.500.12110/paper_00223573_v70_n11_p1561_Michelini |
| _version_ |
1768546055826178048 |