Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n6_p1332_Talarico http://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_Talarico |
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paper:paper_00221317_v92_n6_p1332_Talarico2023-06-08T14:46:34Z Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan Talarico, Laura Beatriz Noseda, Miguel D. Damonte, Elsa Beatriz carrageenan heparan sulfate animal cell antiviral activity article cell proliferation controlled study dengue Dengue virus insect cell mammal cell mosquito nonhuman priority journal protein synthesis Vero cell virus inhibition Animals Carrageenan Cell Line Cercopithecus aethiops Culicidae Dengue Dengue Virus Vero Cells Virus Replication Dengue virus Mammalia The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM. Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Noseda, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n6_p1332_Talarico http://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_Talarico |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carrageenan heparan sulfate animal cell antiviral activity article cell proliferation controlled study dengue Dengue virus insect cell mammal cell mosquito nonhuman priority journal protein synthesis Vero cell virus inhibition Animals Carrageenan Cell Line Cercopithecus aethiops Culicidae Dengue Dengue Virus Vero Cells Virus Replication Dengue virus Mammalia |
| spellingShingle |
carrageenan heparan sulfate animal cell antiviral activity article cell proliferation controlled study dengue Dengue virus insect cell mammal cell mosquito nonhuman priority journal protein synthesis Vero cell virus inhibition Animals Carrageenan Cell Line Cercopithecus aethiops Culicidae Dengue Dengue Virus Vero Cells Virus Replication Dengue virus Mammalia Talarico, Laura Beatriz Noseda, Miguel D. Damonte, Elsa Beatriz Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan |
| topic_facet |
carrageenan heparan sulfate animal cell antiviral activity article cell proliferation controlled study dengue Dengue virus insect cell mammal cell mosquito nonhuman priority journal protein synthesis Vero cell virus inhibition Animals Carrageenan Cell Line Cercopithecus aethiops Culicidae Dengue Dengue Virus Vero Cells Virus Replication Dengue virus Mammalia |
| description |
The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM. |
| author |
Talarico, Laura Beatriz Noseda, Miguel D. Damonte, Elsa Beatriz |
| author_facet |
Talarico, Laura Beatriz Noseda, Miguel D. Damonte, Elsa Beatriz |
| author_sort |
Talarico, Laura Beatriz |
| title |
Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan |
| title_short |
Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan |
| title_full |
Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan |
| title_fullStr |
Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan |
| title_full_unstemmed |
Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan |
| title_sort |
differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n6_p1332_Talarico http://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_Talarico |
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