Characterization of Junin arenavirus cell entry

Junin virus (JUNV) entry is conducted by receptor-mediated endocytosis. To explore the cellular entry mechanism of JUNV, inhibitory effects of drugs affecting the main endocytic pathways on JUNV entry into Vero cells were analysed. Compounds that impair clathrin-mediated endocytosis were shown to re...

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Publicado:	2007
Materias:	clathrin animal cell article caveola cell transport controlled study endocytosis Junin virus lipid raft membrane vesicle nonhuman pathogenesis priority journal transmission electron microscopy transport kinetics Vero cell virogenesis virus cell interaction virus infection virus inhibition virus particle Animals Caveolae Cell Membrane Cercopithecus aethiops Chlorpromazine Clathrin-Coated Vesicles Endocytosis Microscopy, Electron, Transmission Vero Cells Virion Virus Attachment Virus Internalization Arenavirus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v88_n6_p1776_Martinez http://hdl.handle.net/20.500.12110/paper_00221317_v88_n6_p1776_Martinez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00221317_v88_n6_p1776_Martinez
record_format	dspace
spelling	paper:paper_00221317_v88_n6_p1776_Martinez2023-06-08T14:46:32Z Characterization of Junin arenavirus cell entry clathrin animal cell article caveola cell transport controlled study endocytosis Junin virus lipid raft membrane vesicle nonhuman pathogenesis priority journal transmission electron microscopy transport kinetics Vero cell virogenesis virus cell interaction virus infection virus inhibition virus particle Animals Caveolae Cell Membrane Cercopithecus aethiops Chlorpromazine Clathrin-Coated Vesicles Endocytosis Junin virus Microscopy, Electron, Transmission Vero Cells Virion Virus Attachment Virus Internalization Arenavirus Junin virus Junin virus (JUNV) entry is conducted by receptor-mediated endocytosis. To explore the cellular entry mechanism of JUNV, inhibitory effects of drugs affecting the main endocytic pathways on JUNV entry into Vero cells were analysed. Compounds that impair clathrin-mediated endocytosis were shown to reduce virus internalization without affecting virion binding. In contrast, drugs that alter lipid-raft microdomains, impairing caveola-mediated endocytosis, were not able to block virus entry. To show direct evidence of JUNV entry, transmission electron microscopy was performed; it showed JUNV particles of about 60-100 nm in membrane depressions that had an electron-dense coating, In addition, JUNV particles were found within invaginations of the plasma membrane and vesicles that resembled those of pits and clathrin-coated vesicles. Taken together, these results demonstrate that clathrin-mediated endocytosis is the main JUNV entry pathway into Vero cells and represent an important contribution to the characterization of the arenavirus multiplication cycle. © 2007 SGM. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v88_n6_p1776_Martinez http://hdl.handle.net/20.500.12110/paper_00221317_v88_n6_p1776_Martinez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	clathrin animal cell article caveola cell transport controlled study endocytosis Junin virus lipid raft membrane vesicle nonhuman pathogenesis priority journal transmission electron microscopy transport kinetics Vero cell virogenesis virus cell interaction virus infection virus inhibition virus particle Animals Caveolae Cell Membrane Cercopithecus aethiops Chlorpromazine Clathrin-Coated Vesicles Endocytosis Junin virus Microscopy, Electron, Transmission Vero Cells Virion Virus Attachment Virus Internalization Arenavirus Junin virus
spellingShingle	clathrin animal cell article caveola cell transport controlled study endocytosis Junin virus lipid raft membrane vesicle nonhuman pathogenesis priority journal transmission electron microscopy transport kinetics Vero cell virogenesis virus cell interaction virus infection virus inhibition virus particle Animals Caveolae Cell Membrane Cercopithecus aethiops Chlorpromazine Clathrin-Coated Vesicles Endocytosis Junin virus Microscopy, Electron, Transmission Vero Cells Virion Virus Attachment Virus Internalization Arenavirus Junin virus Characterization of Junin arenavirus cell entry
topic_facet	clathrin animal cell article caveola cell transport controlled study endocytosis Junin virus lipid raft membrane vesicle nonhuman pathogenesis priority journal transmission electron microscopy transport kinetics Vero cell virogenesis virus cell interaction virus infection virus inhibition virus particle Animals Caveolae Cell Membrane Cercopithecus aethiops Chlorpromazine Clathrin-Coated Vesicles Endocytosis Junin virus Microscopy, Electron, Transmission Vero Cells Virion Virus Attachment Virus Internalization Arenavirus Junin virus
description	Junin virus (JUNV) entry is conducted by receptor-mediated endocytosis. To explore the cellular entry mechanism of JUNV, inhibitory effects of drugs affecting the main endocytic pathways on JUNV entry into Vero cells were analysed. Compounds that impair clathrin-mediated endocytosis were shown to reduce virus internalization without affecting virion binding. In contrast, drugs that alter lipid-raft microdomains, impairing caveola-mediated endocytosis, were not able to block virus entry. To show direct evidence of JUNV entry, transmission electron microscopy was performed; it showed JUNV particles of about 60-100 nm in membrane depressions that had an electron-dense coating, In addition, JUNV particles were found within invaginations of the plasma membrane and vesicles that resembled those of pits and clathrin-coated vesicles. Taken together, these results demonstrate that clathrin-mediated endocytosis is the main JUNV entry pathway into Vero cells and represent an important contribution to the characterization of the arenavirus multiplication cycle. © 2007 SGM.
title	Characterization of Junin arenavirus cell entry
title_short	Characterization of Junin arenavirus cell entry
title_full	Characterization of Junin arenavirus cell entry
title_fullStr	Characterization of Junin arenavirus cell entry
title_full_unstemmed	Characterization of Junin arenavirus cell entry
title_sort	characterization of junin arenavirus cell entry
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v88_n6_p1776_Martinez http://hdl.handle.net/20.500.12110/paper_00221317_v88_n6_p1776_Martinez
_version_	1768544489478029312

Characterization of Junin arenavirus cell entry

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