Polarized entry and release of Junín virus, a New World arenavirus

Junín virus (JUNV), the causative agent of Argentine haemorrhagic fever, is a human pathogen that naturally enters the body through the epithelial cells of the respiratory and digestive tracts. The interaction of JUNV with two types of polarized epithelial cultures, Vero C1008 and A549, was investig...

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Publicado:	2005
Materias:	colchicine virus glycoprotein virus nucleoprotein animal cell Arenavirus article binding assay cell culture cell membrane transport cell surface confocal microscopy controlled study culture medium epithelium cell human human cell immunofluorescence microscopy intestine epithelium Junin virus microtubule nonhuman polarization priority journal protein expression respiratory epithelium stomach epithelium Vero cell virogenesis virus cell interaction virus hemorrhagic fever virus transmission Animals Cell Membrane Cell Polarity Cells, Cultured Cercopithecus aethiops Epithelial Cells Gene Expression Humans Microscopy, Confocal Nucleocapsid Proteins Vero Cells Arenaviridae Argentina (fish)
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v86_n5_p1475_Cordo http://hdl.handle.net/20.500.12110/paper_00221317_v86_n5_p1475_Cordo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00221317_v86_n5_p1475_Cordo
record_format	dspace
spelling	paper:paper_00221317_v86_n5_p1475_Cordo2023-06-08T14:46:32Z Polarized entry and release of Junín virus, a New World arenavirus colchicine virus glycoprotein virus nucleoprotein animal cell Arenavirus article binding assay cell culture cell membrane transport cell surface confocal microscopy controlled study culture medium epithelium cell human human cell immunofluorescence microscopy intestine epithelium Junin virus microtubule nonhuman polarization priority journal protein expression respiratory epithelium stomach epithelium Vero cell virogenesis virus cell interaction virus hemorrhagic fever virus transmission Animals Cell Membrane Cell Polarity Cells, Cultured Cercopithecus aethiops Epithelial Cells Gene Expression Humans Junin virus Microscopy, Confocal Nucleocapsid Proteins Vero Cells Arenaviridae Arenavirus Argentina (fish) Junin virus Junín virus (JUNV), the causative agent of Argentine haemorrhagic fever, is a human pathogen that naturally enters the body through the epithelial cells of the respiratory and digestive tracts. The interaction of JUNV with two types of polarized epithelial cultures, Vero C1008 and A549, was investigated. Radioactive virus-binding assays showed that JUNV infects polarized lines preferentially through the apical surface. High-level expression of viral nucleoprotein was detected in polarized cell lines infected through the apical domain. Virus production from apical media was about 100-fold higher than that found into the basolateral medium. Confocal-immunofluorescence analysis revealed high-level expression of glycoprotein at the apical-membrane surface. Disruption of the microtubule network by colchicine impaired JUNV vectorial release. This is the first study to analyse the interaction between a member of the virus family Arenaviridae and polarized epithelial cells, showing preferential entry and release from the apical plasma membrane. © 2005 SGM. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v86_n5_p1475_Cordo http://hdl.handle.net/20.500.12110/paper_00221317_v86_n5_p1475_Cordo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	colchicine virus glycoprotein virus nucleoprotein animal cell Arenavirus article binding assay cell culture cell membrane transport cell surface confocal microscopy controlled study culture medium epithelium cell human human cell immunofluorescence microscopy intestine epithelium Junin virus microtubule nonhuman polarization priority journal protein expression respiratory epithelium stomach epithelium Vero cell virogenesis virus cell interaction virus hemorrhagic fever virus transmission Animals Cell Membrane Cell Polarity Cells, Cultured Cercopithecus aethiops Epithelial Cells Gene Expression Humans Junin virus Microscopy, Confocal Nucleocapsid Proteins Vero Cells Arenaviridae Arenavirus Argentina (fish) Junin virus
spellingShingle	colchicine virus glycoprotein virus nucleoprotein animal cell Arenavirus article binding assay cell culture cell membrane transport cell surface confocal microscopy controlled study culture medium epithelium cell human human cell immunofluorescence microscopy intestine epithelium Junin virus microtubule nonhuman polarization priority journal protein expression respiratory epithelium stomach epithelium Vero cell virogenesis virus cell interaction virus hemorrhagic fever virus transmission Animals Cell Membrane Cell Polarity Cells, Cultured Cercopithecus aethiops Epithelial Cells Gene Expression Humans Junin virus Microscopy, Confocal Nucleocapsid Proteins Vero Cells Arenaviridae Arenavirus Argentina (fish) Junin virus Polarized entry and release of Junín virus, a New World arenavirus
topic_facet	colchicine virus glycoprotein virus nucleoprotein animal cell Arenavirus article binding assay cell culture cell membrane transport cell surface confocal microscopy controlled study culture medium epithelium cell human human cell immunofluorescence microscopy intestine epithelium Junin virus microtubule nonhuman polarization priority journal protein expression respiratory epithelium stomach epithelium Vero cell virogenesis virus cell interaction virus hemorrhagic fever virus transmission Animals Cell Membrane Cell Polarity Cells, Cultured Cercopithecus aethiops Epithelial Cells Gene Expression Humans Junin virus Microscopy, Confocal Nucleocapsid Proteins Vero Cells Arenaviridae Arenavirus Argentina (fish) Junin virus
description	Junín virus (JUNV), the causative agent of Argentine haemorrhagic fever, is a human pathogen that naturally enters the body through the epithelial cells of the respiratory and digestive tracts. The interaction of JUNV with two types of polarized epithelial cultures, Vero C1008 and A549, was investigated. Radioactive virus-binding assays showed that JUNV infects polarized lines preferentially through the apical surface. High-level expression of viral nucleoprotein was detected in polarized cell lines infected through the apical domain. Virus production from apical media was about 100-fold higher than that found into the basolateral medium. Confocal-immunofluorescence analysis revealed high-level expression of glycoprotein at the apical-membrane surface. Disruption of the microtubule network by colchicine impaired JUNV vectorial release. This is the first study to analyse the interaction between a member of the virus family Arenaviridae and polarized epithelial cells, showing preferential entry and release from the apical plasma membrane. © 2005 SGM.
title	Polarized entry and release of Junín virus, a New World arenavirus
title_short	Polarized entry and release of Junín virus, a New World arenavirus
title_full	Polarized entry and release of Junín virus, a New World arenavirus
title_fullStr	Polarized entry and release of Junín virus, a New World arenavirus
title_full_unstemmed	Polarized entry and release of Junín virus, a New World arenavirus
title_sort	polarized entry and release of junín virus, a new world arenavirus
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v86_n5_p1475_Cordo http://hdl.handle.net/20.500.12110/paper_00221317_v86_n5_p1475_Cordo
_version_	1768543310296645632

Polarized entry and release of Junín virus, a New World arenavirus

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