Fluctuation of oxidative stress indicators in Salix nigra seeds during priming

Salix nigra seeds subjected to increased humidification show a decrease in normal germination (NG) during early imbibition followed by a recovery in that parameter at increasing imbibition times. Since photo-oxidized seeds contain high levels of reactive oxygen species (ROS), it is possible to infer...

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Detalles Bibliográficos
Autores principales:	Roqueiro, Gonzalo, Maroder, Horacio Luis
Publicado:	2012
Materias:	Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v63_n10_p3631_Roqueiro http://hdl.handle.net/20.500.12110/paper_00220957_v63_n10_p3631_Roqueiro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00220957_v63_n10_p3631_Roqueiro
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spelling	paper:paper_00220957_v63_n10_p3631_Roqueiro2023-06-08T14:45:43Z Fluctuation of oxidative stress indicators in Salix nigra seeds during priming Roqueiro, Gonzalo Maroder, Horacio Luis Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra Salix nigra seeds subjected to increased humidification show a decrease in normal germination (NG) during early imbibition followed by a recovery in that parameter at increasing imbibition times. Since photo-oxidized seeds contain high levels of reactive oxygen species (ROS), it is possible to infer that the atypical decrease in NG is a consequence of a higher ROS mobilization at early imbibition and the subsequent recovery from an increase in antioxidant activity. In this study, several oxidative stress indicators were evaluated in photo-oxidized seeds subjected to priming. ROS production was studied using electronic spin resonance spectroscopy, spontaneous chemiluminescence (SCL), spectrophotometry (with XTT), and histochemical (with DAB and NBT) and cytochemical (with CeCl3) techniques. Four indicators of molecular damage were monitored: lipid peroxidation, pigment destruction, protein oxidation, and membrane integrity. Antioxidant activity was evaluated by changes in the enzymes SOD, CAT, APX, and POX. The results revealed that the decrease in NG at the beginning of priming occurs by an oxidative burst, as determined by increases in both SCL and superoxide anion radical (O2·-). Such oxidative burst generates lipid peroxidation, protein oxidation, and a decrease in both pigment content and enzyme activities. With increasing hydration, damages are progressively reversed and NG restored, which coincides with the increased activity of antioxidant defences. It is proposed that these novel observations regarding the occurrence of an oxidative burst are related to the high basal ROS levels and the high membrane content retained in the mature embryo tissues. © 2012 The Author. Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v63_n10_p3631_Roqueiro http://hdl.handle.net/20.500.12110/paper_00220957_v63_n10_p3631_Roqueiro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra
spellingShingle	Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra Roqueiro, Gonzalo Maroder, Horacio Luis Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
topic_facet	Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra
description	Salix nigra seeds subjected to increased humidification show a decrease in normal germination (NG) during early imbibition followed by a recovery in that parameter at increasing imbibition times. Since photo-oxidized seeds contain high levels of reactive oxygen species (ROS), it is possible to infer that the atypical decrease in NG is a consequence of a higher ROS mobilization at early imbibition and the subsequent recovery from an increase in antioxidant activity. In this study, several oxidative stress indicators were evaluated in photo-oxidized seeds subjected to priming. ROS production was studied using electronic spin resonance spectroscopy, spontaneous chemiluminescence (SCL), spectrophotometry (with XTT), and histochemical (with DAB and NBT) and cytochemical (with CeCl3) techniques. Four indicators of molecular damage were monitored: lipid peroxidation, pigment destruction, protein oxidation, and membrane integrity. Antioxidant activity was evaluated by changes in the enzymes SOD, CAT, APX, and POX. The results revealed that the decrease in NG at the beginning of priming occurs by an oxidative burst, as determined by increases in both SCL and superoxide anion radical (O2·-). Such oxidative burst generates lipid peroxidation, protein oxidation, and a decrease in both pigment content and enzyme activities. With increasing hydration, damages are progressively reversed and NG restored, which coincides with the increased activity of antioxidant defences. It is proposed that these novel observations regarding the occurrence of an oxidative burst are related to the high basal ROS levels and the high membrane content retained in the mature embryo tissues. © 2012 The Author.
author	Roqueiro, Gonzalo Maroder, Horacio Luis
author_facet	Roqueiro, Gonzalo Maroder, Horacio Luis
author_sort	Roqueiro, Gonzalo
title	Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
title_short	Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
title_full	Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
title_fullStr	Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
title_full_unstemmed	Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
title_sort	fluctuation of oxidative stress indicators in salix nigra seeds during priming
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v63_n10_p3631_Roqueiro http://hdl.handle.net/20.500.12110/paper_00220957_v63_n10_p3631_Roqueiro
work_keys_str_mv	AT roqueirogonzalo fluctuationofoxidativestressindicatorsinsalixnigraseedsduringpriming AT maroderhoracioluis fluctuationofoxidativestressindicatorsinsalixnigraseedsduringpriming
_version_	1768545771270963200

Fluctuation of oxidative stress indicators in Salix nigra seeds during priming

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