Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)

The dusky grouper Epinephelus marginatus is a protogynous hermaphrodite fish, that maintains high levels of plasma steroids as juveniles, as substrates for sex inversion. These fish are exposed to marine pollution from oil spills during cargo handling. Polycyclic aromatic hydrocarbons (PAHs), such a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lo Nostro, Fabiana Laura, Da Cuña, Rodrigo Hernán
Publicado:	2018
Materias:	Acute toxicity Gonadal steroids PAH Protogynous hermaphroditism 11 oxotestosterone alcohol estradiol phenanthrene testosterone phenanthrene derivative animal cell animal experiment animal tissue Article concentration (parameters) controlled study drug effect endocrine disease enzyme linked immunosorbent assay Epinephelus Epinephelus marginatus exposure hemosiderosis histology hormone blood level in vitro study in vivo study juvenile animal LC50 nonhuman priority journal spleen hemosiderosis steroidogenesis animal female male metabolism sex determination process Animals Estradiol Female Male Phenanthrenes Sex Determination Processes
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v257_n_p255_deCampos http://hdl.handle.net/20.500.12110/paper_00166480_v257_n_p255_deCampos
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00166480_v257_n_p255_deCampos
record_format	dspace
spelling	paper:paper_00166480_v257_n_p255_deCampos2023-06-08T14:38:22Z Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes) Lo Nostro, Fabiana Laura Da Cuña, Rodrigo Hernán Acute toxicity Gonadal steroids PAH Protogynous hermaphroditism 11 oxotestosterone alcohol estradiol phenanthrene testosterone estradiol phenanthrene phenanthrene derivative animal cell animal experiment animal tissue Article concentration (parameters) controlled study drug effect endocrine disease enzyme linked immunosorbent assay Epinephelus Epinephelus marginatus exposure hemosiderosis histology hormone blood level in vitro study in vivo study juvenile animal LC50 nonhuman priority journal spleen hemosiderosis steroidogenesis animal female male metabolism sex determination process Animals Estradiol Female Male Phenanthrenes Sex Determination Processes The dusky grouper Epinephelus marginatus is a protogynous hermaphrodite fish, that maintains high levels of plasma steroids as juveniles, as substrates for sex inversion. These fish are exposed to marine pollution from oil spills during cargo handling. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene (Phe), are the main crude oil components and are toxic to fish, acting as endocrine disruptors (ED). This is the first study that investigated impacts of Phe as an ED in E. marginatus juveniles. An in vivo sublethal exposure (96 h) to Phe was carried out at two concentrations (0.1 mg/L and 1 mg/L); exposure to the vehicle (ethanol; ETOH) was also performed. Plasma levels of 17β-estradiol (E 2 ), testosterone (T) and 11-ketotestosterone (11-KT) were measured by ELISA. Gonads, liver and spleen were processed for histological analysis. In an in vitro bioassay, gonad fragments were incubated with Phe (8.91 mg/L) or ETOH. Steroid levels in the culture media were measured by ELISA. The in vivo exposure to Phe triggered an increase of the area of the hepatocytes, increased number of melanomacrophagic centers and hemosiderosis in the spleen; ETOH induced similar effects on spleen. E 2 and T levels did not change in plasma or in vitro media. In plasma, ETOH decreased 11-KT levels. Phenanthrene sharply reduced 11-KT levels in vitro. Although in vivo bioassay results were not unequivocal owing to ethanol effects, Phe might disrupt steroidogenesis in juvenile grouper, possibly causing dysfunctions during sex change and gonadal maturity, considering the importance of 11-KT in developing ovaries. © 2017 Elsevier Inc. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Da Cuña, R.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v257_n_p255_deCampos http://hdl.handle.net/20.500.12110/paper_00166480_v257_n_p255_deCampos
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acute toxicity Gonadal steroids PAH Protogynous hermaphroditism 11 oxotestosterone alcohol estradiol phenanthrene testosterone estradiol phenanthrene phenanthrene derivative animal cell animal experiment animal tissue Article concentration (parameters) controlled study drug effect endocrine disease enzyme linked immunosorbent assay Epinephelus Epinephelus marginatus exposure hemosiderosis histology hormone blood level in vitro study in vivo study juvenile animal LC50 nonhuman priority journal spleen hemosiderosis steroidogenesis animal female male metabolism sex determination process Animals Estradiol Female Male Phenanthrenes Sex Determination Processes
spellingShingle	Acute toxicity Gonadal steroids PAH Protogynous hermaphroditism 11 oxotestosterone alcohol estradiol phenanthrene testosterone estradiol phenanthrene phenanthrene derivative animal cell animal experiment animal tissue Article concentration (parameters) controlled study drug effect endocrine disease enzyme linked immunosorbent assay Epinephelus Epinephelus marginatus exposure hemosiderosis histology hormone blood level in vitro study in vivo study juvenile animal LC50 nonhuman priority journal spleen hemosiderosis steroidogenesis animal female male metabolism sex determination process Animals Estradiol Female Male Phenanthrenes Sex Determination Processes Lo Nostro, Fabiana Laura Da Cuña, Rodrigo Hernán Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)
topic_facet	Acute toxicity Gonadal steroids PAH Protogynous hermaphroditism 11 oxotestosterone alcohol estradiol phenanthrene testosterone estradiol phenanthrene phenanthrene derivative animal cell animal experiment animal tissue Article concentration (parameters) controlled study drug effect endocrine disease enzyme linked immunosorbent assay Epinephelus Epinephelus marginatus exposure hemosiderosis histology hormone blood level in vitro study in vivo study juvenile animal LC50 nonhuman priority journal spleen hemosiderosis steroidogenesis animal female male metabolism sex determination process Animals Estradiol Female Male Phenanthrenes Sex Determination Processes
description	The dusky grouper Epinephelus marginatus is a protogynous hermaphrodite fish, that maintains high levels of plasma steroids as juveniles, as substrates for sex inversion. These fish are exposed to marine pollution from oil spills during cargo handling. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene (Phe), are the main crude oil components and are toxic to fish, acting as endocrine disruptors (ED). This is the first study that investigated impacts of Phe as an ED in E. marginatus juveniles. An in vivo sublethal exposure (96 h) to Phe was carried out at two concentrations (0.1 mg/L and 1 mg/L); exposure to the vehicle (ethanol; ETOH) was also performed. Plasma levels of 17β-estradiol (E 2 ), testosterone (T) and 11-ketotestosterone (11-KT) were measured by ELISA. Gonads, liver and spleen were processed for histological analysis. In an in vitro bioassay, gonad fragments were incubated with Phe (8.91 mg/L) or ETOH. Steroid levels in the culture media were measured by ELISA. The in vivo exposure to Phe triggered an increase of the area of the hepatocytes, increased number of melanomacrophagic centers and hemosiderosis in the spleen; ETOH induced similar effects on spleen. E 2 and T levels did not change in plasma or in vitro media. In plasma, ETOH decreased 11-KT levels. Phenanthrene sharply reduced 11-KT levels in vitro. Although in vivo bioassay results were not unequivocal owing to ethanol effects, Phe might disrupt steroidogenesis in juvenile grouper, possibly causing dysfunctions during sex change and gonadal maturity, considering the importance of 11-KT in developing ovaries. © 2017 Elsevier Inc.
author	Lo Nostro, Fabiana Laura Da Cuña, Rodrigo Hernán
author_facet	Lo Nostro, Fabiana Laura Da Cuña, Rodrigo Hernán
author_sort	Lo Nostro, Fabiana Laura
title	Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)
title_short	Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)
title_full	Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)
title_fullStr	Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)
title_full_unstemmed	Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)
title_sort	endocrine disruption of phenanthrene in the protogynous dusky grouper epinephelus marginatus (serranidae: perciformes)
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v257_n_p255_deCampos http://hdl.handle.net/20.500.12110/paper_00166480_v257_n_p255_deCampos
work_keys_str_mv	AT lonostrofabianalaura endocrinedisruptionofphenanthreneintheprotogynousduskygrouperepinephelusmarginatusserranidaeperciformes AT dacunarodrigohernan endocrinedisruptionofphenanthreneintheprotogynousduskygrouperepinephelusmarginatusserranidaeperciformes
_version_	1768544714716348416

Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)

Ejemplares similares