Effect of incubating human sperm at room temperature on capacitation-related events
Objective: To determine the effect of human sperm incubation at room temperature (20°C) upon capacitation-related events. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Semen samples were obtained from normozoospermic volunteers. Human follicular fluid (hFF) was collected...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v77_n2_p252_MarinBriggiler http://hdl.handle.net/20.500.12110/paper_00150282_v77_n2_p252_MarinBriggiler |
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paper:paper_00150282_v77_n2_p252_MarinBriggiler2023-06-08T14:37:53Z Effect of incubating human sperm at room temperature on capacitation-related events Acrosome reaction Human follicular fluid Human spermatozoa Sperm capacitation Temperature phosphoprotein tyrosine acrosome article controlled study follicle fluid human human cell male normal human priority journal prospective study protein phosphorylation room temperature sample sperm spermatozoon spermatozoon capacitation spermatozoon motility temperature volunteer Acrosome Reaction Biomechanics Blotting, Western Culture Media Electrophoresis, Polyacrylamide Gel Female Follicular Fluid Humans Male Phosphorylation Prospective Studies Proteins Sperm Capacitation Sperm Motility Spermatozoa Tyrosine Objective: To determine the effect of human sperm incubation at room temperature (20°C) upon capacitation-related events. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Semen samples were obtained from normozoospermic volunteers. Human follicular fluid (hFF) was collected from women undergoing assisted reproductive treatment.Intervention(s): Spermatozoa were incubated for up to 18 hours at 20°C and/or 37°C. Main Outcome Measure(s): Protein tyrosine phosphorylation patterns, development of hyperactivated motility, and induction of acrosome reaction (AR) in response to hFF. Result(s): Spermatozoa incubated for 18 hours at 20°C showed an array of tyrosine phosphorylated proteins similar to noncapacitated cells. After incubation at 20°C, the percentage of spermatozoa displaying hyperactivated motility and undergoing acrosomal loss in response to hFF was significantly lower when compared with cells kept the same time at 37°C. Conversely, spermatozoa incubated overnight at 37°C could respond to hFF, either at 37°C or 20°C. When preincubation at 20°C was followed by sperm exposure to 37°C, capacitation-related events could be activated. In capacitated cells (16 hours at 37°C), 2-hour incubation at 20°C led to a significant decrease in acrosome reaction inducibility, suggesting sperm decapacitation. Conclusion(s): Human sperm incubation at room temperature does not allow capacitation, although it does not affect hFF-induced acrosome reaction in capacitated cells. The blocking effect is overcome when spermatozoa are exposed to 37°C. Copyright © 2002 American Society for Reproductive Medicine. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v77_n2_p252_MarinBriggiler http://hdl.handle.net/20.500.12110/paper_00150282_v77_n2_p252_MarinBriggiler |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acrosome reaction Human follicular fluid Human spermatozoa Sperm capacitation Temperature phosphoprotein tyrosine acrosome article controlled study follicle fluid human human cell male normal human priority journal prospective study protein phosphorylation room temperature sample sperm spermatozoon spermatozoon capacitation spermatozoon motility temperature volunteer Acrosome Reaction Biomechanics Blotting, Western Culture Media Electrophoresis, Polyacrylamide Gel Female Follicular Fluid Humans Male Phosphorylation Prospective Studies Proteins Sperm Capacitation Sperm Motility Spermatozoa Tyrosine |
| spellingShingle |
Acrosome reaction Human follicular fluid Human spermatozoa Sperm capacitation Temperature phosphoprotein tyrosine acrosome article controlled study follicle fluid human human cell male normal human priority journal prospective study protein phosphorylation room temperature sample sperm spermatozoon spermatozoon capacitation spermatozoon motility temperature volunteer Acrosome Reaction Biomechanics Blotting, Western Culture Media Electrophoresis, Polyacrylamide Gel Female Follicular Fluid Humans Male Phosphorylation Prospective Studies Proteins Sperm Capacitation Sperm Motility Spermatozoa Tyrosine Effect of incubating human sperm at room temperature on capacitation-related events |
| topic_facet |
Acrosome reaction Human follicular fluid Human spermatozoa Sperm capacitation Temperature phosphoprotein tyrosine acrosome article controlled study follicle fluid human human cell male normal human priority journal prospective study protein phosphorylation room temperature sample sperm spermatozoon spermatozoon capacitation spermatozoon motility temperature volunteer Acrosome Reaction Biomechanics Blotting, Western Culture Media Electrophoresis, Polyacrylamide Gel Female Follicular Fluid Humans Male Phosphorylation Prospective Studies Proteins Sperm Capacitation Sperm Motility Spermatozoa Tyrosine |
| description |
Objective: To determine the effect of human sperm incubation at room temperature (20°C) upon capacitation-related events. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Semen samples were obtained from normozoospermic volunteers. Human follicular fluid (hFF) was collected from women undergoing assisted reproductive treatment.Intervention(s): Spermatozoa were incubated for up to 18 hours at 20°C and/or 37°C. Main Outcome Measure(s): Protein tyrosine phosphorylation patterns, development of hyperactivated motility, and induction of acrosome reaction (AR) in response to hFF. Result(s): Spermatozoa incubated for 18 hours at 20°C showed an array of tyrosine phosphorylated proteins similar to noncapacitated cells. After incubation at 20°C, the percentage of spermatozoa displaying hyperactivated motility and undergoing acrosomal loss in response to hFF was significantly lower when compared with cells kept the same time at 37°C. Conversely, spermatozoa incubated overnight at 37°C could respond to hFF, either at 37°C or 20°C. When preincubation at 20°C was followed by sperm exposure to 37°C, capacitation-related events could be activated. In capacitated cells (16 hours at 37°C), 2-hour incubation at 20°C led to a significant decrease in acrosome reaction inducibility, suggesting sperm decapacitation. Conclusion(s): Human sperm incubation at room temperature does not allow capacitation, although it does not affect hFF-induced acrosome reaction in capacitated cells. The blocking effect is overcome when spermatozoa are exposed to 37°C. Copyright © 2002 American Society for Reproductive Medicine. |
| title |
Effect of incubating human sperm at room temperature on capacitation-related events |
| title_short |
Effect of incubating human sperm at room temperature on capacitation-related events |
| title_full |
Effect of incubating human sperm at room temperature on capacitation-related events |
| title_fullStr |
Effect of incubating human sperm at room temperature on capacitation-related events |
| title_full_unstemmed |
Effect of incubating human sperm at room temperature on capacitation-related events |
| title_sort |
effect of incubating human sperm at room temperature on capacitation-related events |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v77_n2_p252_MarinBriggiler http://hdl.handle.net/20.500.12110/paper_00150282_v77_n2_p252_MarinBriggiler |
| _version_ |
1768543307571396608 |