Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin

The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if adde...

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Publicado:	1974
Materias:	colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v86_n2_p295_Medrano http://hdl.handle.net/20.500.12110/paper_00144827_v86_n2_p295_Medrano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00144827_v86_n2_p295_Medrano
record_format	dspace
spelling	paper:paper_00144827_v86_n2_p295_Medrano2023-06-08T14:37:19Z Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if added prior to PHA. Inhibitory concentrations of cytochalasin B were no longer effective in preventing DNA synthesis if added 2 h after exposure to PHA; on the other hand, colchicine and vinblastine were effective even if they were added 16 h after PHA. Studies of lymphocyte aggregation to beads of Sepharose with chemically bound PHA suggest that the effects of these drugs do not seem to lie primarily on blocking PHA binding to the lymphocyte membrane, but rather on a subsequent step(s). © 1974. 1974 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v86_n2_p295_Medrano http://hdl.handle.net/20.500.12110/paper_00144827_v86_n2_p295_Medrano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine
spellingShingle	colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin
topic_facet	colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine
description	The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if added prior to PHA. Inhibitory concentrations of cytochalasin B were no longer effective in preventing DNA synthesis if added 2 h after exposure to PHA; on the other hand, colchicine and vinblastine were effective even if they were added 16 h after PHA. Studies of lymphocyte aggregation to beads of Sepharose with chemically bound PHA suggest that the effects of these drugs do not seem to lie primarily on blocking PHA binding to the lymphocyte membrane, but rather on a subsequent step(s). © 1974.
title	Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin
title_short	Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin
title_full	Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin
title_fullStr	Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin
title_full_unstemmed	Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin
title_sort	effect of colchicine, vinblastine and cytochalasin b on human lymphocyte transformation by phytohemagglutinin
publishDate	1974
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v86_n2_p295_Medrano http://hdl.handle.net/20.500.12110/paper_00144827_v86_n2_p295_Medrano
_version_	1768545768382136320

Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin

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