Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells

Phorbol 12-myristate 13-acetate (PMA), N,N'-hexamethylenebisacetamide (HMBA) and retinoic acid induce cell differentiation in U-937 promonocytic cells. This report examines the effects of these agents on DNA topoisomerase I activity. A decrease in enzyme activity could be detected as early as 3...

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Detalles Bibliográficos
Publicado:	1997
Materias:	Differentiation Topoisomerase I U-937 cell 4alpha phorbol dna topoisomerase hexamethylenebisacetamide phorbol 13 acetate 12 myristate retinoic acid article cell differentiation controlled study enzyme activity human human cell leukemia cell priority journal Acetamides Antineoplastic Agents Blotting, Northern Blotting, Western Carcinogens Cell Transformation, Neoplastic DNA Topoisomerases, Type I Electrophoresis, Polyacrylamide Gel Humans Leukemia, Monocytic, Chronic Tetradecanoylphorbol Acetate Transcription, Genetic Tretinoin Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v324_n1_p129_Shayo http://hdl.handle.net/20.500.12110/paper_00142999_v324_n1_p129_Shayo
Aporte de:	Aportado por : Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires .

id	paper:paper_00142999_v324_n1_p129_Shayo
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Differentiation Topoisomerase I U-937 cell 4alpha phorbol dna topoisomerase hexamethylenebisacetamide phorbol 13 acetate 12 myristate retinoic acid article cell differentiation controlled study enzyme activity human human cell leukemia cell priority journal Acetamides Antineoplastic Agents Blotting, Northern Blotting, Western Carcinogens Cell Transformation, Neoplastic DNA Topoisomerases, Type I Electrophoresis, Polyacrylamide Gel Humans Leukemia, Monocytic, Chronic Tetradecanoylphorbol Acetate Transcription, Genetic Tretinoin Tumor Cells, Cultured
spellingShingle	Differentiation Topoisomerase I U-937 cell 4alpha phorbol dna topoisomerase hexamethylenebisacetamide phorbol 13 acetate 12 myristate retinoic acid article cell differentiation controlled study enzyme activity human human cell leukemia cell priority journal Acetamides Antineoplastic Agents Blotting, Northern Blotting, Western Carcinogens Cell Transformation, Neoplastic DNA Topoisomerases, Type I Electrophoresis, Polyacrylamide Gel Humans Leukemia, Monocytic, Chronic Tetradecanoylphorbol Acetate Transcription, Genetic Tretinoin Tumor Cells, Cultured Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells
topic_facet	Differentiation Topoisomerase I U-937 cell 4alpha phorbol dna topoisomerase hexamethylenebisacetamide phorbol 13 acetate 12 myristate retinoic acid article cell differentiation controlled study enzyme activity human human cell leukemia cell priority journal Acetamides Antineoplastic Agents Blotting, Northern Blotting, Western Carcinogens Cell Transformation, Neoplastic DNA Topoisomerases, Type I Electrophoresis, Polyacrylamide Gel Humans Leukemia, Monocytic, Chronic Tetradecanoylphorbol Acetate Transcription, Genetic Tretinoin Tumor Cells, Cultured
description	Phorbol 12-myristate 13-acetate (PMA), N,N'-hexamethylenebisacetamide (HMBA) and retinoic acid induce cell differentiation in U-937 promonocytic cells. This report examines the effects of these agents on DNA topoisomerase I activity. A decrease in enzyme activity could be detected as early as 30 min after treatment with all three differentiating compounds and lasted at least 48 h. No alteration in the levels of DNA topoisomerase I transcript or protein was observed during these treatments. The results might be explained by post-translational events that render DNA topoisomerase type I less active.
title	Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells
title_short	Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells
title_full	Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells
title_fullStr	Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells
title_full_unstemmed	Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells
title_sort	differentiating agents modulate topoisomerase i activity in u-937 promonocytic cells
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v324_n1_p129_Shayo http://hdl.handle.net/20.500.12110/paper_00142999_v324_n1_p129_Shayo
bdutipo_str	Repositorios
_version_	1764820557158678529

Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells

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