Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells
Phorbol 12-myristate 13-acetate (PMA), N,N'-hexamethylenebisacetamide (HMBA) and retinoic acid induce cell differentiation in U-937 promonocytic cells. This report examines the effects of these agents on DNA topoisomerase I activity. A decrease in enzyme activity could be detected as early as 3...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v324_n1_p129_Shayo http://hdl.handle.net/20.500.12110/paper_00142999_v324_n1_p129_Shayo |
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paper:paper_00142999_v324_n1_p129_Shayo2023-06-08T14:36:50Z Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells Differentiation Topoisomerase I U-937 cell 4alpha phorbol dna topoisomerase hexamethylenebisacetamide phorbol 13 acetate 12 myristate retinoic acid article cell differentiation controlled study enzyme activity human human cell leukemia cell priority journal Acetamides Antineoplastic Agents Blotting, Northern Blotting, Western Carcinogens Cell Transformation, Neoplastic DNA Topoisomerases, Type I Electrophoresis, Polyacrylamide Gel Humans Leukemia, Monocytic, Chronic Tetradecanoylphorbol Acetate Transcription, Genetic Tretinoin Tumor Cells, Cultured Phorbol 12-myristate 13-acetate (PMA), N,N'-hexamethylenebisacetamide (HMBA) and retinoic acid induce cell differentiation in U-937 promonocytic cells. This report examines the effects of these agents on DNA topoisomerase I activity. A decrease in enzyme activity could be detected as early as 30 min after treatment with all three differentiating compounds and lasted at least 48 h. No alteration in the levels of DNA topoisomerase I transcript or protein was observed during these treatments. The results might be explained by post-translational events that render DNA topoisomerase type I less active. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v324_n1_p129_Shayo http://hdl.handle.net/20.500.12110/paper_00142999_v324_n1_p129_Shayo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Differentiation Topoisomerase I U-937 cell 4alpha phorbol dna topoisomerase hexamethylenebisacetamide phorbol 13 acetate 12 myristate retinoic acid article cell differentiation controlled study enzyme activity human human cell leukemia cell priority journal Acetamides Antineoplastic Agents Blotting, Northern Blotting, Western Carcinogens Cell Transformation, Neoplastic DNA Topoisomerases, Type I Electrophoresis, Polyacrylamide Gel Humans Leukemia, Monocytic, Chronic Tetradecanoylphorbol Acetate Transcription, Genetic Tretinoin Tumor Cells, Cultured |
| spellingShingle |
Differentiation Topoisomerase I U-937 cell 4alpha phorbol dna topoisomerase hexamethylenebisacetamide phorbol 13 acetate 12 myristate retinoic acid article cell differentiation controlled study enzyme activity human human cell leukemia cell priority journal Acetamides Antineoplastic Agents Blotting, Northern Blotting, Western Carcinogens Cell Transformation, Neoplastic DNA Topoisomerases, Type I Electrophoresis, Polyacrylamide Gel Humans Leukemia, Monocytic, Chronic Tetradecanoylphorbol Acetate Transcription, Genetic Tretinoin Tumor Cells, Cultured Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells |
| topic_facet |
Differentiation Topoisomerase I U-937 cell 4alpha phorbol dna topoisomerase hexamethylenebisacetamide phorbol 13 acetate 12 myristate retinoic acid article cell differentiation controlled study enzyme activity human human cell leukemia cell priority journal Acetamides Antineoplastic Agents Blotting, Northern Blotting, Western Carcinogens Cell Transformation, Neoplastic DNA Topoisomerases, Type I Electrophoresis, Polyacrylamide Gel Humans Leukemia, Monocytic, Chronic Tetradecanoylphorbol Acetate Transcription, Genetic Tretinoin Tumor Cells, Cultured |
| description |
Phorbol 12-myristate 13-acetate (PMA), N,N'-hexamethylenebisacetamide (HMBA) and retinoic acid induce cell differentiation in U-937 promonocytic cells. This report examines the effects of these agents on DNA topoisomerase I activity. A decrease in enzyme activity could be detected as early as 30 min after treatment with all three differentiating compounds and lasted at least 48 h. No alteration in the levels of DNA topoisomerase I transcript or protein was observed during these treatments. The results might be explained by post-translational events that render DNA topoisomerase type I less active. |
| title |
Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells |
| title_short |
Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells |
| title_full |
Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells |
| title_fullStr |
Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells |
| title_full_unstemmed |
Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells |
| title_sort |
differentiating agents modulate topoisomerase i activity in u-937 promonocytic cells |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v324_n1_p129_Shayo http://hdl.handle.net/20.500.12110/paper_00142999_v324_n1_p129_Shayo |
| _version_ |
1768546426544979968 |