Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides
Nucleic acid immunization is a new vaccination technology. DNA vaccines do not only carry the genetic information for the antigen of interest but also deliver an adjuvant effect due to the presence of immunostimulatory sequences within the plasmid backbone. It is generally assumed that the adjuvant...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142980_v33_n5_p1382_Zelenay http://hdl.handle.net/20.500.12110/paper_00142980_v33_n5_p1382_Zelenay |
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paper:paper_00142980_v33_n5_p1382_Zelenay2023-06-08T14:36:47Z Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides CpG motif DNA vaccine Immunoadjuvanticity antigen cytokine DNA vaccine gamma interferon glycoprotein D interleukin 6 oligodeoxynucleotide derivative plasmid DNA animal cell article B lymphocyte cell activation cell culture cell proliferation cellular immunity controlled study CpG island cytokine production DNA modification DNA strand drug mechanism female humoral immunity immunization immunostimulation in vitro study in vivo study mouse nonhuman priority journal protein motif spleen cell Adjuvants, Immunologic Animals Antigens, CD40 B-Lymphocytes Cytokines Dendritic Cells Female Interferon Type II Killer Cells, Natural Lymphocyte Activation Mice Mice, Inbred BALB C Oligodeoxyribonucleotides Plasmids Thionucleotides Vaccines, DNA Viral Envelope Proteins Nucleic acid immunization is a new vaccination technology. DNA vaccines do not only carry the genetic information for the antigen of interest but also deliver an adjuvant effect due to the presence of immunostimulatory sequences within the plasmid backbone. It is generally assumed that the adjuvant properties of plasmid DNA are equal to those described for oligodeoxynucleotides (ODN) containing immunostimulatory CpG motifs. To challenge this hypothesis we have carried out a series of experiments comparing the ability of single- and double-stranded ODN containing CpG motifs to induce the activation of mouse spleen cells. Moreover, we compared the immunostimulatory properties of plasmids that were modified by the addition of two to four CpG motifs. Our results establish that plasmid DNA express their adjuvanticity as either double or single strands, and no differences were observed between modified and unmodified plasmids. On the other hand, the strongest stimulatory ODN sequences lost their adjuvant properties when administered as double-strand DNA. Furthermore, the profile of cytokines induced on spleen cells by plasmid DNA and ODN is different. Strikingly, plasmid DNA induces a moderate synthesis of IL-6 and a strong synthesis of IFN-γ, whereas stimulation with ODN showed an inverse profile with a higher increase in the synthesis of IL-6 but a moderate increase in IFN-γ. Finally, in vivo studies were consistent with the results obtained in vitro. Mice immunized with modified or unmodified plasmids encoding the glycoprotein D of HSV showed similar levels of cellular and humoral immune responses. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142980_v33_n5_p1382_Zelenay http://hdl.handle.net/20.500.12110/paper_00142980_v33_n5_p1382_Zelenay |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CpG motif DNA vaccine Immunoadjuvanticity antigen cytokine DNA vaccine gamma interferon glycoprotein D interleukin 6 oligodeoxynucleotide derivative plasmid DNA animal cell article B lymphocyte cell activation cell culture cell proliferation cellular immunity controlled study CpG island cytokine production DNA modification DNA strand drug mechanism female humoral immunity immunization immunostimulation in vitro study in vivo study mouse nonhuman priority journal protein motif spleen cell Adjuvants, Immunologic Animals Antigens, CD40 B-Lymphocytes Cytokines Dendritic Cells Female Interferon Type II Killer Cells, Natural Lymphocyte Activation Mice Mice, Inbred BALB C Oligodeoxyribonucleotides Plasmids Thionucleotides Vaccines, DNA Viral Envelope Proteins |
| spellingShingle |
CpG motif DNA vaccine Immunoadjuvanticity antigen cytokine DNA vaccine gamma interferon glycoprotein D interleukin 6 oligodeoxynucleotide derivative plasmid DNA animal cell article B lymphocyte cell activation cell culture cell proliferation cellular immunity controlled study CpG island cytokine production DNA modification DNA strand drug mechanism female humoral immunity immunization immunostimulation in vitro study in vivo study mouse nonhuman priority journal protein motif spleen cell Adjuvants, Immunologic Animals Antigens, CD40 B-Lymphocytes Cytokines Dendritic Cells Female Interferon Type II Killer Cells, Natural Lymphocyte Activation Mice Mice, Inbred BALB C Oligodeoxyribonucleotides Plasmids Thionucleotides Vaccines, DNA Viral Envelope Proteins Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides |
| topic_facet |
CpG motif DNA vaccine Immunoadjuvanticity antigen cytokine DNA vaccine gamma interferon glycoprotein D interleukin 6 oligodeoxynucleotide derivative plasmid DNA animal cell article B lymphocyte cell activation cell culture cell proliferation cellular immunity controlled study CpG island cytokine production DNA modification DNA strand drug mechanism female humoral immunity immunization immunostimulation in vitro study in vivo study mouse nonhuman priority journal protein motif spleen cell Adjuvants, Immunologic Animals Antigens, CD40 B-Lymphocytes Cytokines Dendritic Cells Female Interferon Type II Killer Cells, Natural Lymphocyte Activation Mice Mice, Inbred BALB C Oligodeoxyribonucleotides Plasmids Thionucleotides Vaccines, DNA Viral Envelope Proteins |
| description |
Nucleic acid immunization is a new vaccination technology. DNA vaccines do not only carry the genetic information for the antigen of interest but also deliver an adjuvant effect due to the presence of immunostimulatory sequences within the plasmid backbone. It is generally assumed that the adjuvant properties of plasmid DNA are equal to those described for oligodeoxynucleotides (ODN) containing immunostimulatory CpG motifs. To challenge this hypothesis we have carried out a series of experiments comparing the ability of single- and double-stranded ODN containing CpG motifs to induce the activation of mouse spleen cells. Moreover, we compared the immunostimulatory properties of plasmids that were modified by the addition of two to four CpG motifs. Our results establish that plasmid DNA express their adjuvanticity as either double or single strands, and no differences were observed between modified and unmodified plasmids. On the other hand, the strongest stimulatory ODN sequences lost their adjuvant properties when administered as double-strand DNA. Furthermore, the profile of cytokines induced on spleen cells by plasmid DNA and ODN is different. Strikingly, plasmid DNA induces a moderate synthesis of IL-6 and a strong synthesis of IFN-γ, whereas stimulation with ODN showed an inverse profile with a higher increase in the synthesis of IL-6 but a moderate increase in IFN-γ. Finally, in vivo studies were consistent with the results obtained in vitro. Mice immunized with modified or unmodified plasmids encoding the glycoprotein D of HSV showed similar levels of cellular and humoral immune responses. |
| title |
Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides |
| title_short |
Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides |
| title_full |
Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides |
| title_fullStr |
Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides |
| title_full_unstemmed |
Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides |
| title_sort |
immunostimulatory effects of plasmid dna and synthetic oligodeoxynucleotides |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142980_v33_n5_p1382_Zelenay http://hdl.handle.net/20.500.12110/paper_00142980_v33_n5_p1382_Zelenay |
| _version_ |
1768546522700447744 |