Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin
The antiapoptotic effect of melatonin has been described in several systems. In this study, the antagonistic effect of the methoxyindole on dexamethasone-induced apoptosis in mouse thymocytes was examined. Melatonin decreased both DNA fragmentation, and the number of annexin V-positive cells incubat...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v145_n1_p418_Hoijman http://hdl.handle.net/20.500.12110/paper_00137227_v145_n1_p418_Hoijman |
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paper:paper_00137227_v145_n1_p418_Hoijman2023-06-08T14:36:11Z Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin Hoijman, Esteban Rocha Viegas, Luciana Rosenstein, Ruth Estela Pecci, Adali cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland The antiapoptotic effect of melatonin has been described in several systems. In this study, the antagonistic effect of the methoxyindole on dexamethasone-induced apoptosis in mouse thymocytes was examined. Melatonin decreased both DNA fragmentation, and the number of annexin V-positive cells incubated in the presence of dexamethasone. Analysis of the expression of the members of the Bcl-2 family indicated that the synthetic glucocorticoid increased Bax protein levels without affecting the levels of Bcl-2, Bcl-X L, Bcl-X S, or Bak. This effect correlated with an increase in thymocytes bax mRNA levels. Dexamethasone also increased the release of cytochrome C from mitochondria. All of these effects were reduced in the presence of melatonin, which was ineffective per se on these parameters. In addition, the involvement of cAMP on glucocorticoid/melatonin antagonism was examined. Both melatonin and dexamethasone decreased the levels of this nucleotide in mouse thymocytes, indicating that the antagonistic action between both hormones involves a cAMP-independent pathway. In summary, the present results suggest that the antiapoptotic effect of melatonin on glucocorticoid-treated thymocytes would be a consequence of an inhibition of the mitochondrial pathway, presumably through the regulation of Bax protein levels. Fil:Hoijman, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocha Viegas, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosenstein, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v145_n1_p418_Hoijman http://hdl.handle.net/20.500.12110/paper_00137227_v145_n1_p418_Hoijman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland |
| spellingShingle |
cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland Hoijman, Esteban Rocha Viegas, Luciana Rosenstein, Ruth Estela Pecci, Adali Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin |
| topic_facet |
cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland |
| description |
The antiapoptotic effect of melatonin has been described in several systems. In this study, the antagonistic effect of the methoxyindole on dexamethasone-induced apoptosis in mouse thymocytes was examined. Melatonin decreased both DNA fragmentation, and the number of annexin V-positive cells incubated in the presence of dexamethasone. Analysis of the expression of the members of the Bcl-2 family indicated that the synthetic glucocorticoid increased Bax protein levels without affecting the levels of Bcl-2, Bcl-X L, Bcl-X S, or Bak. This effect correlated with an increase in thymocytes bax mRNA levels. Dexamethasone also increased the release of cytochrome C from mitochondria. All of these effects were reduced in the presence of melatonin, which was ineffective per se on these parameters. In addition, the involvement of cAMP on glucocorticoid/melatonin antagonism was examined. Both melatonin and dexamethasone decreased the levels of this nucleotide in mouse thymocytes, indicating that the antagonistic action between both hormones involves a cAMP-independent pathway. In summary, the present results suggest that the antiapoptotic effect of melatonin on glucocorticoid-treated thymocytes would be a consequence of an inhibition of the mitochondrial pathway, presumably through the regulation of Bax protein levels. |
| author |
Hoijman, Esteban Rocha Viegas, Luciana Rosenstein, Ruth Estela Pecci, Adali |
| author_facet |
Hoijman, Esteban Rocha Viegas, Luciana Rosenstein, Ruth Estela Pecci, Adali |
| author_sort |
Hoijman, Esteban |
| title |
Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin |
| title_short |
Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin |
| title_full |
Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin |
| title_fullStr |
Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin |
| title_full_unstemmed |
Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin |
| title_sort |
involvement of bax protein in the prevention of glucocorticoid-induced thymocytes apoptosis by melatonin |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v145_n1_p418_Hoijman http://hdl.handle.net/20.500.12110/paper_00137227_v145_n1_p418_Hoijman |
| work_keys_str_mv |
AT hoijmanesteban involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin AT rochaviegasluciana involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin AT rosensteinruthestela involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin AT pecciadali involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin |
| _version_ |
1768545309622796288 |