Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes

The effects of the carrageenans 1T1 (λ-type), 1C1 (κ/ι-type) and 1C3 (ιι/ν-type), isolated from the red seaweed Gigartina skottsbergii, on herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection of murine astrocytes were investigated. The three compounds were effective inhibitors of virus r...

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Publicado: 1999
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v45_n6_p429_Carlucci
http://hdl.handle.net/20.500.12110/paper_00093157_v45_n6_p429_Carlucci
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spelling paper:paper_00093157_v45_n6_p429_Carlucci2023-06-08T14:34:00Z Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes Antiviral properties Astrocytes Carrageenans Herpes simplex virus carrageenan glial fibrillary acidic protein animal cell article astrocyte Herpes simplex virus 1 Herpes simplex virus 2 nonhuman priority journal Vero cell virus inhibition virus replication The effects of the carrageenans 1T1 (λ-type), 1C1 (κ/ι-type) and 1C3 (ιι/ν-type), isolated from the red seaweed Gigartina skottsbergii, on herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection of murine astrocytes were investigated. The three compounds were effective inhibitors of virus replication, as determined by a virus yield inhibition assay, in astrocytes as well as in Vero cells. The 50% inhibitory concentration was in the range of 0.9-3.6 and 0.4-3.2 μg/ml for astrocytes and Vero cells, respectively, whereas the 90% inhibitory concentration ranged from 6.5 to 17.0 μg/ml in astrocytes and from 3.5 to 22.2 μg/ml in Vero cells. No cytotoxicity was detected at concentrations of up to 1000 μg/ml, indicating high selectivity indices for these compounds. Inhibition of viral cytopathology and antigen expression was also detected in the presence of the carrageenans. The increase in the expression of glial fibrillary acidic protein (GFAP), an activated astrocyte marker, produced during the course of HSV-1 infection in astrocytes, was reversed in the presence of 1C1 and 1C3. By contrast, the λ-carrageenan 1T1 increased the expression of GFAP, independently of HSV-1 infection. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v45_n6_p429_Carlucci http://hdl.handle.net/20.500.12110/paper_00093157_v45_n6_p429_Carlucci
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antiviral properties
Astrocytes
Carrageenans
Herpes simplex virus
carrageenan
glial fibrillary acidic protein
animal cell
article
astrocyte
Herpes simplex virus 1
Herpes simplex virus 2
nonhuman
priority journal
Vero cell
virus inhibition
virus replication
spellingShingle Antiviral properties
Astrocytes
Carrageenans
Herpes simplex virus
carrageenan
glial fibrillary acidic protein
animal cell
article
astrocyte
Herpes simplex virus 1
Herpes simplex virus 2
nonhuman
priority journal
Vero cell
virus inhibition
virus replication
Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes
topic_facet Antiviral properties
Astrocytes
Carrageenans
Herpes simplex virus
carrageenan
glial fibrillary acidic protein
animal cell
article
astrocyte
Herpes simplex virus 1
Herpes simplex virus 2
nonhuman
priority journal
Vero cell
virus inhibition
virus replication
description The effects of the carrageenans 1T1 (λ-type), 1C1 (κ/ι-type) and 1C3 (ιι/ν-type), isolated from the red seaweed Gigartina skottsbergii, on herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection of murine astrocytes were investigated. The three compounds were effective inhibitors of virus replication, as determined by a virus yield inhibition assay, in astrocytes as well as in Vero cells. The 50% inhibitory concentration was in the range of 0.9-3.6 and 0.4-3.2 μg/ml for astrocytes and Vero cells, respectively, whereas the 90% inhibitory concentration ranged from 6.5 to 17.0 μg/ml in astrocytes and from 3.5 to 22.2 μg/ml in Vero cells. No cytotoxicity was detected at concentrations of up to 1000 μg/ml, indicating high selectivity indices for these compounds. Inhibition of viral cytopathology and antigen expression was also detected in the presence of the carrageenans. The increase in the expression of glial fibrillary acidic protein (GFAP), an activated astrocyte marker, produced during the course of HSV-1 infection in astrocytes, was reversed in the presence of 1C1 and 1C3. By contrast, the λ-carrageenan 1T1 increased the expression of GFAP, independently of HSV-1 infection.
title Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes
title_short Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes
title_full Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes
title_fullStr Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes
title_full_unstemmed Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes
title_sort inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes
publishDate 1999
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v45_n6_p429_Carlucci
http://hdl.handle.net/20.500.12110/paper_00093157_v45_n6_p429_Carlucci
_version_ 1768542390843342848