Modulation of the immune response mediated by oral transgene administration of IL-10

In the current study, we analyze the immunomodulatory effect of oral transgene administration of IL-10 using a mice model of viral inflammation. Salmonella harboring a plasmid encoding the IL-10 gene (SLIL10) was administered by the oral route together with Salmonella carrying a plasmid encoding the...

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Publicado:	2002
Materias:	DNA immunization HSV IL-10 Immune suppression Salmonella gamma interferon glycoprotein B glycoprotein D interleukin 10 interleukin 2 virus antibody virus protein antigen presentation article bacterial strain cellular immunity controlled study disease model drug effect female genetic code Herpes simplex virus 2 humoral immunity immunization immunomodulation lymphocyte proliferation mouse nonhuman plasmid priority journal protein synthesis transgene vaginitis virus infection Administration, Oral Animals Antibody Formation Disease Models, Animal Down-Regulation Female Herpes Genitalis Herpes Simplex Herpesvirus 2, Human Immunity, Cellular Injections, Intramuscular Interleukin-10 Mice Mice, Inbred BALB C Plasmids Transformation, Bacterial Transgenes Vaccination Vaccines, Synthetic Viral Envelope Proteins Human herpesvirus 2 Simplexvirus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00088749_v216_n1-2_p73_Elias http://hdl.handle.net/20.500.12110/paper_00088749_v216_n1-2_p73_Elias
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00088749_v216_n1-2_p73_Elias
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spelling	paper:paper_00088749_v216_n1-2_p73_Elias2023-06-08T14:33:24Z Modulation of the immune response mediated by oral transgene administration of IL-10 DNA immunization HSV IL-10 Immune suppression Salmonella gamma interferon glycoprotein B glycoprotein D interleukin 10 interleukin 2 virus antibody virus protein antigen presentation article bacterial strain cellular immunity controlled study disease model drug effect female genetic code Herpes simplex virus 2 humoral immunity immunization immunomodulation lymphocyte proliferation mouse nonhuman plasmid priority journal protein synthesis Salmonella transgene vaginitis virus infection Administration, Oral Animals Antibody Formation Disease Models, Animal Down-Regulation Female Herpes Genitalis Herpes Simplex Herpesvirus 2, Human Immunity, Cellular Injections, Intramuscular Interleukin-10 Mice Mice, Inbred BALB C Plasmids Salmonella Transformation, Bacterial Transgenes Vaccination Vaccines, Synthetic Viral Envelope Proteins Human herpesvirus 2 Salmonella Simplexvirus In the current study, we analyze the immunomodulatory effect of oral transgene administration of IL-10 using a mice model of viral inflammation. Salmonella harboring a plasmid encoding the IL-10 gene (SLIL10) was administered by the oral route together with Salmonella carrying a plasmid encoding the glycoprotein D or B (SLgD, SLgB) of Herpes simplex virus type 2 (HSV-2). This resulted in a high inhibition of the cellular and human immune response against the viral proteins. When mice immunized against the HSV proteins were challenged with 10 lethal doses of HSV-2 by the intravaginal route, only those that had also received SLIL10 showed severe lesions and died. When Salmonella harboring pIL10 was administered orally to mice immunized by the intramuscular route with a plasmid encoding gD, inhibition of cellular and humoral immune responses were also observed but to a lesser extent than with oral immunization. By means of adoptive transfer experiments and in vitro experiments, we have subsequently determined that the mechanism possibly involved in the inhibition of the immune response could be a reduced antigenic presentation when mice receive SLIL10 that induced a state of anergy on specific T lymphocytes. © 2002 Elsevier Science (USA). All rights reserved. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00088749_v216_n1-2_p73_Elias http://hdl.handle.net/20.500.12110/paper_00088749_v216_n1-2_p73_Elias
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	DNA immunization HSV IL-10 Immune suppression Salmonella gamma interferon glycoprotein B glycoprotein D interleukin 10 interleukin 2 virus antibody virus protein antigen presentation article bacterial strain cellular immunity controlled study disease model drug effect female genetic code Herpes simplex virus 2 humoral immunity immunization immunomodulation lymphocyte proliferation mouse nonhuman plasmid priority journal protein synthesis Salmonella transgene vaginitis virus infection Administration, Oral Animals Antibody Formation Disease Models, Animal Down-Regulation Female Herpes Genitalis Herpes Simplex Herpesvirus 2, Human Immunity, Cellular Injections, Intramuscular Interleukin-10 Mice Mice, Inbred BALB C Plasmids Salmonella Transformation, Bacterial Transgenes Vaccination Vaccines, Synthetic Viral Envelope Proteins Human herpesvirus 2 Salmonella Simplexvirus
spellingShingle	DNA immunization HSV IL-10 Immune suppression Salmonella gamma interferon glycoprotein B glycoprotein D interleukin 10 interleukin 2 virus antibody virus protein antigen presentation article bacterial strain cellular immunity controlled study disease model drug effect female genetic code Herpes simplex virus 2 humoral immunity immunization immunomodulation lymphocyte proliferation mouse nonhuman plasmid priority journal protein synthesis Salmonella transgene vaginitis virus infection Administration, Oral Animals Antibody Formation Disease Models, Animal Down-Regulation Female Herpes Genitalis Herpes Simplex Herpesvirus 2, Human Immunity, Cellular Injections, Intramuscular Interleukin-10 Mice Mice, Inbred BALB C Plasmids Salmonella Transformation, Bacterial Transgenes Vaccination Vaccines, Synthetic Viral Envelope Proteins Human herpesvirus 2 Salmonella Simplexvirus Modulation of the immune response mediated by oral transgene administration of IL-10
topic_facet	DNA immunization HSV IL-10 Immune suppression Salmonella gamma interferon glycoprotein B glycoprotein D interleukin 10 interleukin 2 virus antibody virus protein antigen presentation article bacterial strain cellular immunity controlled study disease model drug effect female genetic code Herpes simplex virus 2 humoral immunity immunization immunomodulation lymphocyte proliferation mouse nonhuman plasmid priority journal protein synthesis Salmonella transgene vaginitis virus infection Administration, Oral Animals Antibody Formation Disease Models, Animal Down-Regulation Female Herpes Genitalis Herpes Simplex Herpesvirus 2, Human Immunity, Cellular Injections, Intramuscular Interleukin-10 Mice Mice, Inbred BALB C Plasmids Salmonella Transformation, Bacterial Transgenes Vaccination Vaccines, Synthetic Viral Envelope Proteins Human herpesvirus 2 Salmonella Simplexvirus
description	In the current study, we analyze the immunomodulatory effect of oral transgene administration of IL-10 using a mice model of viral inflammation. Salmonella harboring a plasmid encoding the IL-10 gene (SLIL10) was administered by the oral route together with Salmonella carrying a plasmid encoding the glycoprotein D or B (SLgD, SLgB) of Herpes simplex virus type 2 (HSV-2). This resulted in a high inhibition of the cellular and human immune response against the viral proteins. When mice immunized against the HSV proteins were challenged with 10 lethal doses of HSV-2 by the intravaginal route, only those that had also received SLIL10 showed severe lesions and died. When Salmonella harboring pIL10 was administered orally to mice immunized by the intramuscular route with a plasmid encoding gD, inhibition of cellular and humoral immune responses were also observed but to a lesser extent than with oral immunization. By means of adoptive transfer experiments and in vitro experiments, we have subsequently determined that the mechanism possibly involved in the inhibition of the immune response could be a reduced antigenic presentation when mice receive SLIL10 that induced a state of anergy on specific T lymphocytes. © 2002 Elsevier Science (USA). All rights reserved.
title	Modulation of the immune response mediated by oral transgene administration of IL-10
title_short	Modulation of the immune response mediated by oral transgene administration of IL-10
title_full	Modulation of the immune response mediated by oral transgene administration of IL-10
title_fullStr	Modulation of the immune response mediated by oral transgene administration of IL-10
title_full_unstemmed	Modulation of the immune response mediated by oral transgene administration of IL-10
title_sort	modulation of the immune response mediated by oral transgene administration of il-10
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00088749_v216_n1-2_p73_Elias http://hdl.handle.net/20.500.12110/paper_00088749_v216_n1-2_p73_Elias
_version_	1768546050213150720

Modulation of the immune response mediated by oral transgene administration of IL-10

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