ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures

In spite of the wide range of tumours successfully treated with 5-aminolevulinic acid mediated photodynamic therapy, the fact that 5-aminolevulinic acid has low lipid solubility, limits its clinical application. More lipophilic 5-aminolevulinic acid prodrugs and the use of liposomal carriers are two...

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Autores principales:	Casas, Adriana Gabriela, Perotti, Christian Pablo, Sacca, Paula Alejandra, Fukuda, Haydeé, Batlle, Alcira María del Carmen
Publicado:	2002
Materias:	ALA ALA derivatives Aminolevulinic acid Hexyl-ALA Liposomes PDT Photodynamic therapy aminolevulinic acid liposome porphyrin prodrug animal cell animal experiment animal model animal tissue article cell damage cell membrane controlled study drug formulation encapsulation endocytosis endoplasmic reticulum laser lipophilicity male mouse nonhuman organ culture phospholipid vesicle photodynamic therapy photosensitivity priority journal protein localization protein structure tumor cell culture Aminolevulinic Acid Animals Male Mice Mice, Inbred BALB C Microscopy, Electron Mitochondria Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v86_n5_p837_Casas http://hdl.handle.net/20.500.12110/paper_00070920_v86_n5_p837_Casas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00070920_v86_n5_p837_Casas
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spelling	paper:paper_00070920_v86_n5_p837_Casas2025-07-30T17:14:42Z ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures Casas, Adriana Gabriela Perotti, Christian Pablo Sacca, Paula Alejandra Fukuda, Haydeé Batlle, Alcira María del Carmen ALA ALA derivatives Aminolevulinic acid Hexyl-ALA Liposomes PDT Photodynamic therapy aminolevulinic acid liposome porphyrin prodrug animal cell animal experiment animal model animal tissue article cell damage cell membrane controlled study drug formulation encapsulation endocytosis endoplasmic reticulum laser lipophilicity male mouse nonhuman organ culture phospholipid vesicle photodynamic therapy photosensitivity priority journal protein localization protein structure tumor cell culture Aminolevulinic Acid Animals Liposomes Male Mice Mice, Inbred BALB C Microscopy, Electron Mitochondria Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured In spite of the wide range of tumours successfully treated with 5-aminolevulinic acid mediated photodynamic therapy, the fact that 5-aminolevulinic acid has low lipid solubility, limits its clinical application. More lipophilic 5-aminolevulinic acid prodrugs and the use of liposomal carriers are two approaches aimed at improving 5-aminolevulinic acid transmembrane access. In this study we used both 5-aminolevulinic acid and its hexyl ester in their free and encapsulated formulations to compare their corresponding endogenous synthesis of porphyrins. Employing murine tumour cultures, we found that neither the use of hexyl ester nor the entrappment of either 5-aminolevulinic acid or hexyl ester into liposomes increase the rate of tumour porphyrin synthesis. By light and electronic microscopy it was demonstrated that exposure of tumour explants to either free or liposomal 5-aminolevulinic acid and subsequent illumination induces the same type of subcellullar damage. Mitochondria, endoplasmic reticulum and plasma membrane are the structures mostly injured in the early steps of photodynamic treatment. In a later stage, cytoplasmic and nuclear disintegration are observed. By electronic microscopy the involvement of the endocytic pathway in the incorporation of liposomal 5-aminolevulinic acid into the cells was shown. © 2002 Cancer Research UK. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sacca, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v86_n5_p837_Casas http://hdl.handle.net/20.500.12110/paper_00070920_v86_n5_p837_Casas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ALA ALA derivatives Aminolevulinic acid Hexyl-ALA Liposomes PDT Photodynamic therapy aminolevulinic acid liposome porphyrin prodrug animal cell animal experiment animal model animal tissue article cell damage cell membrane controlled study drug formulation encapsulation endocytosis endoplasmic reticulum laser lipophilicity male mouse nonhuman organ culture phospholipid vesicle photodynamic therapy photosensitivity priority journal protein localization protein structure tumor cell culture Aminolevulinic Acid Animals Liposomes Male Mice Mice, Inbred BALB C Microscopy, Electron Mitochondria Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
spellingShingle	ALA ALA derivatives Aminolevulinic acid Hexyl-ALA Liposomes PDT Photodynamic therapy aminolevulinic acid liposome porphyrin prodrug animal cell animal experiment animal model animal tissue article cell damage cell membrane controlled study drug formulation encapsulation endocytosis endoplasmic reticulum laser lipophilicity male mouse nonhuman organ culture phospholipid vesicle photodynamic therapy photosensitivity priority journal protein localization protein structure tumor cell culture Aminolevulinic Acid Animals Liposomes Male Mice Mice, Inbred BALB C Microscopy, Electron Mitochondria Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured Casas, Adriana Gabriela Perotti, Christian Pablo Sacca, Paula Alejandra Fukuda, Haydeé Batlle, Alcira María del Carmen ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures
topic_facet	ALA ALA derivatives Aminolevulinic acid Hexyl-ALA Liposomes PDT Photodynamic therapy aminolevulinic acid liposome porphyrin prodrug animal cell animal experiment animal model animal tissue article cell damage cell membrane controlled study drug formulation encapsulation endocytosis endoplasmic reticulum laser lipophilicity male mouse nonhuman organ culture phospholipid vesicle photodynamic therapy photosensitivity priority journal protein localization protein structure tumor cell culture Aminolevulinic Acid Animals Liposomes Male Mice Mice, Inbred BALB C Microscopy, Electron Mitochondria Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
description	In spite of the wide range of tumours successfully treated with 5-aminolevulinic acid mediated photodynamic therapy, the fact that 5-aminolevulinic acid has low lipid solubility, limits its clinical application. More lipophilic 5-aminolevulinic acid prodrugs and the use of liposomal carriers are two approaches aimed at improving 5-aminolevulinic acid transmembrane access. In this study we used both 5-aminolevulinic acid and its hexyl ester in their free and encapsulated formulations to compare their corresponding endogenous synthesis of porphyrins. Employing murine tumour cultures, we found that neither the use of hexyl ester nor the entrappment of either 5-aminolevulinic acid or hexyl ester into liposomes increase the rate of tumour porphyrin synthesis. By light and electronic microscopy it was demonstrated that exposure of tumour explants to either free or liposomal 5-aminolevulinic acid and subsequent illumination induces the same type of subcellullar damage. Mitochondria, endoplasmic reticulum and plasma membrane are the structures mostly injured in the early steps of photodynamic treatment. In a later stage, cytoplasmic and nuclear disintegration are observed. By electronic microscopy the involvement of the endocytic pathway in the incorporation of liposomal 5-aminolevulinic acid into the cells was shown. © 2002 Cancer Research UK.
author	Casas, Adriana Gabriela Perotti, Christian Pablo Sacca, Paula Alejandra Fukuda, Haydeé Batlle, Alcira María del Carmen
author_facet	Casas, Adriana Gabriela Perotti, Christian Pablo Sacca, Paula Alejandra Fukuda, Haydeé Batlle, Alcira María del Carmen
author_sort	Casas, Adriana Gabriela
title	ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures
title_short	ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures
title_full	ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures
title_fullStr	ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures
title_full_unstemmed	ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures
title_sort	ala and ala hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v86_n5_p837_Casas http://hdl.handle.net/20.500.12110/paper_00070920_v86_n5_p837_Casas
work_keys_str_mv	AT casasadrianagabriela alaandalahexylesterinfreeandliposomalformulationsforthephotosensitisationoftumourorgancultures AT perottichristianpablo alaandalahexylesterinfreeandliposomalformulationsforthephotosensitisationoftumourorgancultures AT saccapaulaalejandra alaandalahexylesterinfreeandliposomalformulationsforthephotosensitisationoftumourorgancultures AT fukudahaydee alaandalahexylesterinfreeandliposomalformulationsforthephotosensitisationoftumourorgancultures AT batllealciramariadelcarmen alaandalahexylesterinfreeandliposomalformulationsforthephotosensitisationoftumourorgancultures
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ALA and ALA hexyl ester in free and liposomal formulations for the photosensitisation of tumour organ cultures

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