A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients

Local intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual channels or clusters of channels. To gain a mechanistic understanding of these events we need to know the magnitude and spatial distribution of the underlying Ca2+ flux. However, this is difficult to infer fro...

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Autores principales:	Ventura, Alejandra C., Bruno, Luciana, Ponce Dawson, Silvina
Publicado:	2005
Materias:	adenosine triphosphatase (calcium) calcium channel calcium ion voltage gated calcium channel algorithm article calcium binding calcium cell level calcium current calcium signaling calcium transport cytosol diffusion fluorescence mathematical analysis model
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063495_v88_n4_p2403_Ventura http://hdl.handle.net/20.500.12110/paper_00063495_v88_n4_p2403_Ventura
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00063495_v88_n4_p2403_Ventura
record_format	dspace
spelling	paper:paper_00063495_v88_n4_p2403_Ventura2023-06-08T14:31:17Z A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients Ventura, Alejandra C. Bruno, Luciana Ponce Dawson, Silvina adenosine triphosphatase (calcium) calcium channel calcium ion voltage gated calcium channel algorithm article calcium binding calcium cell level calcium current calcium signaling calcium transport cytosol diffusion fluorescence mathematical analysis model Local intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual channels or clusters of channels. To gain a mechanistic understanding of these events we need to know the magnitude and spatial distribution of the underlying Ca2+ flux. However, this is difficult to infer from fluorescence Ca2+ images because the distribution of Ca2+-bound dye is affected by poorly characterized processes including diffusion of Ca2+ ions, their binding to mobile and immobile buffers, and sequestration by Ca2+ pumps. Several methods have previously been proposed to derive Ca2+ flux from fluorescence images, but all require explicit knowledge or assumptions regarding these processes. We now present a novel algorithm that requires few assumptions and is largely model-independent. By testing the algorithm with both numerically generated image data and experimental images of sparklets resulting from Ca2+ flux through individual voltage-gated channels, we show that it satisfactorily reconstructs the magnitude and time course of the underlying Ca2+ currents. © 2005 by the Biophysical Society. Fil:Ventura, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dawson, S.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063495_v88_n4_p2403_Ventura http://hdl.handle.net/20.500.12110/paper_00063495_v88_n4_p2403_Ventura
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	adenosine triphosphatase (calcium) calcium channel calcium ion voltage gated calcium channel algorithm article calcium binding calcium cell level calcium current calcium signaling calcium transport cytosol diffusion fluorescence mathematical analysis model
spellingShingle	adenosine triphosphatase (calcium) calcium channel calcium ion voltage gated calcium channel algorithm article calcium binding calcium cell level calcium current calcium signaling calcium transport cytosol diffusion fluorescence mathematical analysis model Ventura, Alejandra C. Bruno, Luciana Ponce Dawson, Silvina A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients
topic_facet	adenosine triphosphatase (calcium) calcium channel calcium ion voltage gated calcium channel algorithm article calcium binding calcium cell level calcium current calcium signaling calcium transport cytosol diffusion fluorescence mathematical analysis model
description	Local intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual channels or clusters of channels. To gain a mechanistic understanding of these events we need to know the magnitude and spatial distribution of the underlying Ca2+ flux. However, this is difficult to infer from fluorescence Ca2+ images because the distribution of Ca2+-bound dye is affected by poorly characterized processes including diffusion of Ca2+ ions, their binding to mobile and immobile buffers, and sequestration by Ca2+ pumps. Several methods have previously been proposed to derive Ca2+ flux from fluorescence images, but all require explicit knowledge or assumptions regarding these processes. We now present a novel algorithm that requires few assumptions and is largely model-independent. By testing the algorithm with both numerically generated image data and experimental images of sparklets resulting from Ca2+ flux through individual voltage-gated channels, we show that it satisfactorily reconstructs the magnitude and time course of the underlying Ca2+ currents. © 2005 by the Biophysical Society.
author	Ventura, Alejandra C. Bruno, Luciana Ponce Dawson, Silvina
author_facet	Ventura, Alejandra C. Bruno, Luciana Ponce Dawson, Silvina
author_sort	Ventura, Alejandra C.
title	A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients
title_short	A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients
title_full	A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients
title_fullStr	A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients
title_full_unstemmed	A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients
title_sort	model-independent algorithm to derive ca2+ fluxes underlying local cytosolic ca2+ transients
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063495_v88_n4_p2403_Ventura http://hdl.handle.net/20.500.12110/paper_00063495_v88_n4_p2403_Ventura
work_keys_str_mv	AT venturaalejandrac amodelindependentalgorithmtoderiveca2fluxesunderlyinglocalcytosolicca2transients AT brunoluciana amodelindependentalgorithmtoderiveca2fluxesunderlyinglocalcytosolicca2transients AT poncedawsonsilvina amodelindependentalgorithmtoderiveca2fluxesunderlyinglocalcytosolicca2transients AT venturaalejandrac modelindependentalgorithmtoderiveca2fluxesunderlyinglocalcytosolicca2transients AT brunoluciana modelindependentalgorithmtoderiveca2fluxesunderlyinglocalcytosolicca2transients AT poncedawsonsilvina modelindependentalgorithmtoderiveca2fluxesunderlyinglocalcytosolicca2transients
_version_	1768543113859563520

A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients

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