Intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of BCL2 related genes
The angiopoietin (ANGPT) receptor (TEK) system plays a crucial role in blood vessel development and regression. To date, no reports have addressed the actions of the anti-ANGPT1 antibody on gonadotropin-stimulated follicular development and atresia in the ovary. Therefore, in this study we specifica...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v78_n3_p506_Parborell http://hdl.handle.net/20.500.12110/paper_00063363_v78_n3_p506_Parborell |
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paper:paper_00063363_v78_n3_p506_Parborell2023-06-08T14:31:08Z Intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of BCL2 related genes Parborell, M.Fernanda A. Abramovich, Dalhia Nurit Tesone, Marta Angiogenesis Angiopoietin 1 Apoptosis Follicle Ovary angiopoietin 1 angiopoietin 1 antibody Fas antigen Fas ligand gonadotropin inhibitor of apoptosis protein protein antibody protein Bax protein bcl 2 protein bcl x unclassified drug angiogenesis animal cell animal experiment animal model apoptosis article cell culture controlled study culture medium DNA fragmentation gene expression granulosa cell nonhuman ovary ovary follicle ovary follicle atresia ovary follicle development paracrine signaling prepuberty priority journal protein blood level protein expression protein family protein function rat theca cell Angiopoietin-1 Animals Antibodies Apoptosis Cell Count DNA Fragmentation Endothelium Female Gene Expression Regulation, Developmental Gonadotropins Injections, Intraperitoneal Ovarian Follicle Peritoneal Cavity Proto-Oncogene Proteins c-bcl-2 Rats Rats, Sprague-Dawley Sexual Maturation Rattus The angiopoietin (ANGPT) receptor (TEK) system plays a crucial role in blood vessel development and regression. To date, no reports have addressed the actions of the anti-ANGPT1 antibody on gonadotropin-stimulated follicular development and atresia in the ovary. Therefore, in this study we specifically investigated whether ANGPT1 plays a critical intraovarian survival role for gonadotropin-dependent folliculogenesis. In particular, we examined the effect of local administration of anti-ANGPT1 antibody on follicular development, apoptosis, and expression of BCL2 protein family members (BAX, BCL2, and BCL2L1), TNFRSF6, and FASLG in ovarian follicles from prepubertal eCG-treated rats. The inhibition of ANGPT1 caused an increase in the number of atretic follicles and a decrease in the number of both antral follicles (AFs) and preovulatory follicles in gonadotropin-treated rat ovaries. Taking into account that follicular atresia is mediated by apoptosis, we analyzed the effect of the antibody against ANGPT1 on programmed cell death. The inhibition of the action of ANGPT1 caused an increase both in the number of apoptotic granulosa cells in AFs and in the spontaneous DNA fragmentation of AFs cultured in serum-free medium. Besides, AFs obtained from rats treated with intraovarian antibodies against ANGPT1 showed both a decrease in BCL2 and an increase in BAX protein levels. Moreover, a reduction in the BCL2L1L/BCL2L1S ratio was observed in this group, with a reduction of BCL2L1L greater than that of BCL2L1S, thus showing that the expression of these antiapoptotic proteins is lower in follicles from treated rats than in those from untreated ones. Our findings suggest that the inhibition of ANGPT1 activity causes an increase in the number of atretic follicles mediated by ovarian apoptosis through an imbalance in the ratio of antiapoptotic to proapoptotic proteins. This could take place through a paracrine effect on granulosa cells mediated by the TEK receptor in theca cells. Therefore, these data clearly indicate that ANGPT1 is necessary for follicular development induced by gonadotropins. © 2008 by the Society for the Study of Reproduction, Inc. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Abramovich, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v78_n3_p506_Parborell http://hdl.handle.net/20.500.12110/paper_00063363_v78_n3_p506_Parborell |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Angiogenesis Angiopoietin 1 Apoptosis Follicle Ovary angiopoietin 1 angiopoietin 1 antibody Fas antigen Fas ligand gonadotropin inhibitor of apoptosis protein protein antibody protein Bax protein bcl 2 protein bcl x unclassified drug angiogenesis animal cell animal experiment animal model apoptosis article cell culture controlled study culture medium DNA fragmentation gene expression granulosa cell nonhuman ovary ovary follicle ovary follicle atresia ovary follicle development paracrine signaling prepuberty priority journal protein blood level protein expression protein family protein function rat theca cell Angiopoietin-1 Animals Antibodies Apoptosis Cell Count DNA Fragmentation Endothelium Female Gene Expression Regulation, Developmental Gonadotropins Injections, Intraperitoneal Ovarian Follicle Peritoneal Cavity Proto-Oncogene Proteins c-bcl-2 Rats Rats, Sprague-Dawley Sexual Maturation Rattus |
| spellingShingle |
Angiogenesis Angiopoietin 1 Apoptosis Follicle Ovary angiopoietin 1 angiopoietin 1 antibody Fas antigen Fas ligand gonadotropin inhibitor of apoptosis protein protein antibody protein Bax protein bcl 2 protein bcl x unclassified drug angiogenesis animal cell animal experiment animal model apoptosis article cell culture controlled study culture medium DNA fragmentation gene expression granulosa cell nonhuman ovary ovary follicle ovary follicle atresia ovary follicle development paracrine signaling prepuberty priority journal protein blood level protein expression protein family protein function rat theca cell Angiopoietin-1 Animals Antibodies Apoptosis Cell Count DNA Fragmentation Endothelium Female Gene Expression Regulation, Developmental Gonadotropins Injections, Intraperitoneal Ovarian Follicle Peritoneal Cavity Proto-Oncogene Proteins c-bcl-2 Rats Rats, Sprague-Dawley Sexual Maturation Rattus Parborell, M.Fernanda A. Abramovich, Dalhia Nurit Tesone, Marta Intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of BCL2 related genes |
| topic_facet |
Angiogenesis Angiopoietin 1 Apoptosis Follicle Ovary angiopoietin 1 angiopoietin 1 antibody Fas antigen Fas ligand gonadotropin inhibitor of apoptosis protein protein antibody protein Bax protein bcl 2 protein bcl x unclassified drug angiogenesis animal cell animal experiment animal model apoptosis article cell culture controlled study culture medium DNA fragmentation gene expression granulosa cell nonhuman ovary ovary follicle ovary follicle atresia ovary follicle development paracrine signaling prepuberty priority journal protein blood level protein expression protein family protein function rat theca cell Angiopoietin-1 Animals Antibodies Apoptosis Cell Count DNA Fragmentation Endothelium Female Gene Expression Regulation, Developmental Gonadotropins Injections, Intraperitoneal Ovarian Follicle Peritoneal Cavity Proto-Oncogene Proteins c-bcl-2 Rats Rats, Sprague-Dawley Sexual Maturation Rattus |
| description |
The angiopoietin (ANGPT) receptor (TEK) system plays a crucial role in blood vessel development and regression. To date, no reports have addressed the actions of the anti-ANGPT1 antibody on gonadotropin-stimulated follicular development and atresia in the ovary. Therefore, in this study we specifically investigated whether ANGPT1 plays a critical intraovarian survival role for gonadotropin-dependent folliculogenesis. In particular, we examined the effect of local administration of anti-ANGPT1 antibody on follicular development, apoptosis, and expression of BCL2 protein family members (BAX, BCL2, and BCL2L1), TNFRSF6, and FASLG in ovarian follicles from prepubertal eCG-treated rats. The inhibition of ANGPT1 caused an increase in the number of atretic follicles and a decrease in the number of both antral follicles (AFs) and preovulatory follicles in gonadotropin-treated rat ovaries. Taking into account that follicular atresia is mediated by apoptosis, we analyzed the effect of the antibody against ANGPT1 on programmed cell death. The inhibition of the action of ANGPT1 caused an increase both in the number of apoptotic granulosa cells in AFs and in the spontaneous DNA fragmentation of AFs cultured in serum-free medium. Besides, AFs obtained from rats treated with intraovarian antibodies against ANGPT1 showed both a decrease in BCL2 and an increase in BAX protein levels. Moreover, a reduction in the BCL2L1L/BCL2L1S ratio was observed in this group, with a reduction of BCL2L1L greater than that of BCL2L1S, thus showing that the expression of these antiapoptotic proteins is lower in follicles from treated rats than in those from untreated ones. Our findings suggest that the inhibition of ANGPT1 activity causes an increase in the number of atretic follicles mediated by ovarian apoptosis through an imbalance in the ratio of antiapoptotic to proapoptotic proteins. This could take place through a paracrine effect on granulosa cells mediated by the TEK receptor in theca cells. Therefore, these data clearly indicate that ANGPT1 is necessary for follicular development induced by gonadotropins. © 2008 by the Society for the Study of Reproduction, Inc. |
| author |
Parborell, M.Fernanda A. Abramovich, Dalhia Nurit Tesone, Marta |
| author_facet |
Parborell, M.Fernanda A. Abramovich, Dalhia Nurit Tesone, Marta |
| author_sort |
Parborell, M.Fernanda A. |
| title |
Intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of BCL2 related genes |
| title_short |
Intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of BCL2 related genes |
| title_full |
Intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of BCL2 related genes |
| title_fullStr |
Intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of BCL2 related genes |
| title_full_unstemmed |
Intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of BCL2 related genes |
| title_sort |
intrabursal administration of the antiangiopoietin 1 antibody produces a delay in rat follicular development associated with an increase in ovarian apoptosis mediated by changes in the expression of bcl2 related genes |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v78_n3_p506_Parborell http://hdl.handle.net/20.500.12110/paper_00063363_v78_n3_p506_Parborell |
| work_keys_str_mv |
AT parborellmfernandaa intrabursaladministrationoftheantiangiopoietin1antibodyproducesadelayinratfolliculardevelopmentassociatedwithanincreaseinovarianapoptosismediatedbychangesintheexpressionofbcl2relatedgenes AT abramovichdalhianurit intrabursaladministrationoftheantiangiopoietin1antibodyproducesadelayinratfolliculardevelopmentassociatedwithanincreaseinovarianapoptosismediatedbychangesintheexpressionofbcl2relatedgenes AT tesonemarta intrabursaladministrationoftheantiangiopoietin1antibodyproducesadelayinratfolliculardevelopmentassociatedwithanincreaseinovarianapoptosismediatedbychangesintheexpressionofbcl2relatedgenes |
| _version_ |
1768542672219275264 |