Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development

Catecholestrogens are endogenous metabolites that have been shown to modulate granulosa, theca, and luteal cell function in some species. The present study was aimed at determining the possible role of these steroids on oocyte maturation. Cumulus-enclosed bovine oocytes were matured for 24 h, fertil...

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Publicado: 2003
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v69_n6_p1793_Lattanzi
http://hdl.handle.net/20.500.12110/paper_00063363_v69_n6_p1793_Lattanzi
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spelling paper:paper_00063363_v69_n6_p1793_Lattanzi2023-06-08T14:31:05Z Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development Embryo Gamete biology Meiosis Oocyte development Steroid hormones 2 hydroxyestradiol 2 methoxyestradiol 4 hydroxyestradiol aromatic hydrocarbon receptor catechol estrogen colchicine estradiol ligand roscovitine steroid tubulin animal cell blastocyst cattle chromosome aberration conference paper controlled study cumulus oophorus embryo culture embryo development female fertilization gamete in vitro study male meiosis metabolite microtubule assembly nonhuman oocyte cleavage oocyte maturation ovary follicle fluid priority journal Animals Antioxidants Apoptosis Blastocyst Cattle Cells, Cultured Colchicine Cytogenetic Analysis Dose-Response Relationship, Drug Estradiol Female Fertilization in Vitro Male Meiosis Mitotic Spindle Apparatus Oocytes Animalia Bos taurus Bovinae Mammalia Catecholestrogens are endogenous metabolites that have been shown to modulate granulosa, theca, and luteal cell function in some species. The present study was aimed at determining the possible role of these steroids on oocyte maturation. Cumulus-enclosed bovine oocytes were matured for 24 h, fertilized, and then cultured for 8 days. Whereas estradiol was without effect, addition of catecholestrogens (2-hydroxyestradiol, 4-hydroxyestradiol, and 2-methoxyestradiol [2-MOE2]) to the maturation medium did not affect the cleavage rate but was associated with a decrease in blastocyst production on Day 8. Although 2-MOE2 was also able to inhibit blastocyst formation when added during embryo culture, the effects were less pronounced than those seen when the steroid was added only during maturation. In agreement with the known ability of 2-MOE2 to bind tubulin at the colchicine site, marked alterations were observed in the spindle assembly of oocytes exposed to 2-MOE2 during maturation, which lead to gross chromosomal aberrations after fertilization and consequent developmental arrest at the morula stage. Moreover, that the blastocyst rate was not affected when meiosis was blocked with roscovitine during 2-MOE2 exposure is consistent with the idea that altered nuclear maturation is the cause of the low developmental competence. Because 2-MOE2 could be increased in follicular fluid in response to aryl hydrocarbon-receptor ligands, such as some environmental contaminants, our results show that abnormally high intraovarian levels of catecholestrogens could have a deleterious effect on oocyte maturation and early embryonic development arising from the alterations in the meiotic spindle. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v69_n6_p1793_Lattanzi http://hdl.handle.net/20.500.12110/paper_00063363_v69_n6_p1793_Lattanzi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Embryo
Gamete biology
Meiosis
Oocyte development
Steroid hormones
2 hydroxyestradiol
2 methoxyestradiol
4 hydroxyestradiol
aromatic hydrocarbon receptor
catechol estrogen
colchicine
estradiol
ligand
roscovitine
steroid
tubulin
animal cell
blastocyst
cattle
chromosome aberration
conference paper
controlled study
cumulus oophorus
embryo culture
embryo development
female
fertilization
gamete
in vitro study
male
meiosis
metabolite
microtubule assembly
nonhuman
oocyte cleavage
oocyte maturation
ovary follicle fluid
priority journal
Animals
Antioxidants
Apoptosis
Blastocyst
Cattle
Cells, Cultured
Colchicine
Cytogenetic Analysis
Dose-Response Relationship, Drug
Estradiol
Female
Fertilization in Vitro
Male
Meiosis
Mitotic Spindle Apparatus
Oocytes
Animalia
Bos taurus
Bovinae
Mammalia
spellingShingle Embryo
Gamete biology
Meiosis
Oocyte development
Steroid hormones
2 hydroxyestradiol
2 methoxyestradiol
4 hydroxyestradiol
aromatic hydrocarbon receptor
catechol estrogen
colchicine
estradiol
ligand
roscovitine
steroid
tubulin
animal cell
blastocyst
cattle
chromosome aberration
conference paper
controlled study
cumulus oophorus
embryo culture
embryo development
female
fertilization
gamete
in vitro study
male
meiosis
metabolite
microtubule assembly
nonhuman
oocyte cleavage
oocyte maturation
ovary follicle fluid
priority journal
Animals
Antioxidants
Apoptosis
Blastocyst
Cattle
Cells, Cultured
Colchicine
Cytogenetic Analysis
Dose-Response Relationship, Drug
Estradiol
Female
Fertilization in Vitro
Male
Meiosis
Mitotic Spindle Apparatus
Oocytes
Animalia
Bos taurus
Bovinae
Mammalia
Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development
topic_facet Embryo
Gamete biology
Meiosis
Oocyte development
Steroid hormones
2 hydroxyestradiol
2 methoxyestradiol
4 hydroxyestradiol
aromatic hydrocarbon receptor
catechol estrogen
colchicine
estradiol
ligand
roscovitine
steroid
tubulin
animal cell
blastocyst
cattle
chromosome aberration
conference paper
controlled study
cumulus oophorus
embryo culture
embryo development
female
fertilization
gamete
in vitro study
male
meiosis
metabolite
microtubule assembly
nonhuman
oocyte cleavage
oocyte maturation
ovary follicle fluid
priority journal
Animals
Antioxidants
Apoptosis
Blastocyst
Cattle
Cells, Cultured
Colchicine
Cytogenetic Analysis
Dose-Response Relationship, Drug
Estradiol
Female
Fertilization in Vitro
Male
Meiosis
Mitotic Spindle Apparatus
Oocytes
Animalia
Bos taurus
Bovinae
Mammalia
description Catecholestrogens are endogenous metabolites that have been shown to modulate granulosa, theca, and luteal cell function in some species. The present study was aimed at determining the possible role of these steroids on oocyte maturation. Cumulus-enclosed bovine oocytes were matured for 24 h, fertilized, and then cultured for 8 days. Whereas estradiol was without effect, addition of catecholestrogens (2-hydroxyestradiol, 4-hydroxyestradiol, and 2-methoxyestradiol [2-MOE2]) to the maturation medium did not affect the cleavage rate but was associated with a decrease in blastocyst production on Day 8. Although 2-MOE2 was also able to inhibit blastocyst formation when added during embryo culture, the effects were less pronounced than those seen when the steroid was added only during maturation. In agreement with the known ability of 2-MOE2 to bind tubulin at the colchicine site, marked alterations were observed in the spindle assembly of oocytes exposed to 2-MOE2 during maturation, which lead to gross chromosomal aberrations after fertilization and consequent developmental arrest at the morula stage. Moreover, that the blastocyst rate was not affected when meiosis was blocked with roscovitine during 2-MOE2 exposure is consistent with the idea that altered nuclear maturation is the cause of the low developmental competence. Because 2-MOE2 could be increased in follicular fluid in response to aryl hydrocarbon-receptor ligands, such as some environmental contaminants, our results show that abnormally high intraovarian levels of catecholestrogens could have a deleterious effect on oocyte maturation and early embryonic development arising from the alterations in the meiotic spindle.
title Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development
title_short Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development
title_full Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development
title_fullStr Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development
title_full_unstemmed Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development
title_sort exposure of bovine oocytes to the endogenous metabolite 2-methoxyestradiol during in vitro maturation inhibits early embryonic development
publishDate 2003
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v69_n6_p1793_Lattanzi
http://hdl.handle.net/20.500.12110/paper_00063363_v69_n6_p1793_Lattanzi
_version_ 1768544347244986368