Expression of pejerrey gonadotropin-releasing hormone in three orders of fish
Molecular variants of GnRH were characterized by reversephase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [Synbranchus marmoratus]), Cyprinidontiformes (platyfish [Xiphophorus maculatus] and green swordtail [X. helleri),...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v67_n6_p1864_Somoza http://hdl.handle.net/20.500.12110/paper_00063363_v67_n6_p1864_Somoza |
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paper:paper_00063363_v67_n6_p1864_Somoza2023-06-08T14:31:04Z Expression of pejerrey gonadotropin-releasing hormone in three orders of fish Anterior pituitary Central nervous system Gonadotropin-releasing hormone Hypothalamic hormones Neuropeptides brain extract gonadorelin gonadorelin tissue extract animal tissue article chicken controlled study female fish hypophysis hypophysis function male nonhuman priority journal radioimmunoassay reversed phase high performance liquid chromatography salmon animal brain level chemistry Cyprinodontiformes gene expression genetics high performance liquid chromatography isolation and purification metabolism Non-programmatic Smegmamorpha Anguilliformes Animalia Atheriniformes Gallus gallus Monopterus albus Odontesthes bonariensis Synbranchiformes Synbranchus marmoratus Xiphophorus hellerii Xiphophorus maculatus Non-programmatic Animals Brain Chemistry Chromatography, High Pressure Liquid Cyprinodontiformes Female Gene Expression Gonadotropin-Releasing Hormone Male Pituitary Gland Smegmamorpha Tissue Extracts Molecular variants of GnRH were characterized by reversephase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [Synbranchus marmoratus]), Cyprinidontiformes (platyfish [Xiphophorus maculatus] and green swordtail [X. helleri), and Atheriniformes (Patagonia pejerrey [Odontesthes hatchery]. Also, pituitary gland extracts from the pejerrey O. bonariensis (Atheriniformes) were characterized. Eluted fractions were tested in radioimmunoassays with antisera specific to GnRH, including both antisera that detected only one form of GnRH and those that detected several forms. The results show that brain extracts obtained from all species contained the same three molecular forms of GnRH, which were immunologically and chromatographically undistinguishable from chicken GnRH-II, pejerrey GnRH (pjGnRH), and salmon GnRH. This study supports the hypothesis that expression of these three forms is common in different fish orders and that pjGnRH is the main regulator of pituitary function in these fish. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v67_n6_p1864_Somoza http://hdl.handle.net/20.500.12110/paper_00063363_v67_n6_p1864_Somoza |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anterior pituitary Central nervous system Gonadotropin-releasing hormone Hypothalamic hormones Neuropeptides brain extract gonadorelin gonadorelin tissue extract animal tissue article chicken controlled study female fish hypophysis hypophysis function male nonhuman priority journal radioimmunoassay reversed phase high performance liquid chromatography salmon animal brain level chemistry Cyprinodontiformes gene expression genetics high performance liquid chromatography isolation and purification metabolism Non-programmatic Smegmamorpha Anguilliformes Animalia Atheriniformes Gallus gallus Monopterus albus Odontesthes bonariensis Synbranchiformes Synbranchus marmoratus Xiphophorus hellerii Xiphophorus maculatus Non-programmatic Animals Brain Chemistry Chromatography, High Pressure Liquid Cyprinodontiformes Female Gene Expression Gonadotropin-Releasing Hormone Male Pituitary Gland Smegmamorpha Tissue Extracts |
| spellingShingle |
Anterior pituitary Central nervous system Gonadotropin-releasing hormone Hypothalamic hormones Neuropeptides brain extract gonadorelin gonadorelin tissue extract animal tissue article chicken controlled study female fish hypophysis hypophysis function male nonhuman priority journal radioimmunoassay reversed phase high performance liquid chromatography salmon animal brain level chemistry Cyprinodontiformes gene expression genetics high performance liquid chromatography isolation and purification metabolism Non-programmatic Smegmamorpha Anguilliformes Animalia Atheriniformes Gallus gallus Monopterus albus Odontesthes bonariensis Synbranchiformes Synbranchus marmoratus Xiphophorus hellerii Xiphophorus maculatus Non-programmatic Animals Brain Chemistry Chromatography, High Pressure Liquid Cyprinodontiformes Female Gene Expression Gonadotropin-Releasing Hormone Male Pituitary Gland Smegmamorpha Tissue Extracts Expression of pejerrey gonadotropin-releasing hormone in three orders of fish |
| topic_facet |
Anterior pituitary Central nervous system Gonadotropin-releasing hormone Hypothalamic hormones Neuropeptides brain extract gonadorelin gonadorelin tissue extract animal tissue article chicken controlled study female fish hypophysis hypophysis function male nonhuman priority journal radioimmunoassay reversed phase high performance liquid chromatography salmon animal brain level chemistry Cyprinodontiformes gene expression genetics high performance liquid chromatography isolation and purification metabolism Non-programmatic Smegmamorpha Anguilliformes Animalia Atheriniformes Gallus gallus Monopterus albus Odontesthes bonariensis Synbranchiformes Synbranchus marmoratus Xiphophorus hellerii Xiphophorus maculatus Non-programmatic Animals Brain Chemistry Chromatography, High Pressure Liquid Cyprinodontiformes Female Gene Expression Gonadotropin-Releasing Hormone Male Pituitary Gland Smegmamorpha Tissue Extracts |
| description |
Molecular variants of GnRH were characterized by reversephase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [Synbranchus marmoratus]), Cyprinidontiformes (platyfish [Xiphophorus maculatus] and green swordtail [X. helleri), and Atheriniformes (Patagonia pejerrey [Odontesthes hatchery]. Also, pituitary gland extracts from the pejerrey O. bonariensis (Atheriniformes) were characterized. Eluted fractions were tested in radioimmunoassays with antisera specific to GnRH, including both antisera that detected only one form of GnRH and those that detected several forms. The results show that brain extracts obtained from all species contained the same three molecular forms of GnRH, which were immunologically and chromatographically undistinguishable from chicken GnRH-II, pejerrey GnRH (pjGnRH), and salmon GnRH. This study supports the hypothesis that expression of these three forms is common in different fish orders and that pjGnRH is the main regulator of pituitary function in these fish. |
| title |
Expression of pejerrey gonadotropin-releasing hormone in three orders of fish |
| title_short |
Expression of pejerrey gonadotropin-releasing hormone in three orders of fish |
| title_full |
Expression of pejerrey gonadotropin-releasing hormone in three orders of fish |
| title_fullStr |
Expression of pejerrey gonadotropin-releasing hormone in three orders of fish |
| title_full_unstemmed |
Expression of pejerrey gonadotropin-releasing hormone in three orders of fish |
| title_sort |
expression of pejerrey gonadotropin-releasing hormone in three orders of fish |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v67_n6_p1864_Somoza http://hdl.handle.net/20.500.12110/paper_00063363_v67_n6_p1864_Somoza |
| _version_ |
1768541683666911232 |