GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process

Yeast cells are able to adapt their metabolism according to the quality of both carbon and nitrogen sources available in the environment. Saccharomyces cerevisiae UGA4 gene encodes a permease capable of transporting γ-aminobutyric acid (GABA) into the cells. Yeast uses this amino acid as a nitrogen...

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Autores principales: Cardillo, Sabrina Beatriz, Bertotti, Santiago Andrés, Correa García, Susana Raquel
Publicado: 2012
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v421_n3_p572_Levi
http://hdl.handle.net/20.500.12110/paper_0006291X_v421_n3_p572_Levi
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spelling paper:paper_0006291X_v421_n3_p572_Levi2023-06-08T14:30:19Z GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process Cardillo, Sabrina Beatriz Bertotti, Santiago Andrés Correa García, Susana Raquel Carbon regulation Dal81 GATA factors Kinases Uga3 UGA4 4 aminobutyric acid carbon fungal protein transcription factor uga4 protein unclassified drug article carbon source gene induction nonhuman priority journal protein expression protein localization protein phosphorylation Saccharomyces cerevisiae Carbon DNA-Binding Proteins Fermentation GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Glucose Phosphatidylinositol 3-Kinases Promoter Regions, Genetic Protein-Serine-Threonine Kinases Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae Yeast cells are able to adapt their metabolism according to the quality of both carbon and nitrogen sources available in the environment. Saccharomyces cerevisiae UGA4 gene encodes a permease capable of transporting γ-aminobutyric acid (GABA) into the cells. Yeast uses this amino acid as a nitrogen source or as a carbon skeleton that enters the tricarboxylic acid cycle.The quality of the carbon source modulates UGA4 expression through two parallel pathways, each one acting on different regulatory elements, the UAS GATA and the UAS GABA. In the presence of a fermentable carbon source, UGA4 expression is induced by GABA while in the presence of a non-fermentable carbon source this expression is GABA-independent.The aim of this work was to study the mechanisms responsible for the differences in the profiles of UGA4 expression in both growth conditions.We found that although the subcellular localization of Gln3 depends on the carbon source and UGA4 expression depends on Tor1 and Snf1, Gln3 localization does not depend on these kinases. We also found that the phosphorylation of Gln3 is mediated by two systems activated by a non-fermentable carbon source, involving the Snf1 kinase and an unidentified TORC1-regulated kinase.We also found that the activity of the main transcription factors responsible for UGA4 induction by GABA varies depending on the quality of the carbon source. In a fermentable carbon source such as glucose, the negative GATA factor Dal80 binds to UGA4 promoter; only after the addition of the inducer, the positive factors Uga3, Dal81 and Gln3 interact with the promoter removing Dal80 and leading to gene induction. In contrast, in the non-fermentable carbon source acetate the negative GATA factor remains bound to UGA4 promoter in the presence or absence of GABA, the positive factors are not detected bound in any of these conditions and in consequence, UGA4 is not induced. © 2012 Elsevier Inc. Fil:Cardillo, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bertotti, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v421_n3_p572_Levi http://hdl.handle.net/20.500.12110/paper_0006291X_v421_n3_p572_Levi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Carbon regulation
Dal81
GATA factors
Kinases
Uga3
UGA4
4 aminobutyric acid
carbon
fungal protein
transcription factor
uga4 protein
unclassified drug
article
carbon source
gene induction
nonhuman
priority journal
protein expression
protein localization
protein phosphorylation
Saccharomyces cerevisiae
Carbon
DNA-Binding Proteins
Fermentation
GABA Plasma Membrane Transport Proteins
gamma-Aminobutyric Acid
Gene Expression Regulation, Developmental
Gene Expression Regulation, Fungal
Glucose
Phosphatidylinositol 3-Kinases
Promoter Regions, Genetic
Protein-Serine-Threonine Kinases
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Saccharomyces cerevisiae
spellingShingle Carbon regulation
Dal81
GATA factors
Kinases
Uga3
UGA4
4 aminobutyric acid
carbon
fungal protein
transcription factor
uga4 protein
unclassified drug
article
carbon source
gene induction
nonhuman
priority journal
protein expression
protein localization
protein phosphorylation
Saccharomyces cerevisiae
Carbon
DNA-Binding Proteins
Fermentation
GABA Plasma Membrane Transport Proteins
gamma-Aminobutyric Acid
Gene Expression Regulation, Developmental
Gene Expression Regulation, Fungal
Glucose
Phosphatidylinositol 3-Kinases
Promoter Regions, Genetic
Protein-Serine-Threonine Kinases
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Saccharomyces cerevisiae
Cardillo, Sabrina Beatriz
Bertotti, Santiago Andrés
Correa García, Susana Raquel
GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
topic_facet Carbon regulation
Dal81
GATA factors
Kinases
Uga3
UGA4
4 aminobutyric acid
carbon
fungal protein
transcription factor
uga4 protein
unclassified drug
article
carbon source
gene induction
nonhuman
priority journal
protein expression
protein localization
protein phosphorylation
Saccharomyces cerevisiae
Carbon
DNA-Binding Proteins
Fermentation
GABA Plasma Membrane Transport Proteins
gamma-Aminobutyric Acid
Gene Expression Regulation, Developmental
Gene Expression Regulation, Fungal
Glucose
Phosphatidylinositol 3-Kinases
Promoter Regions, Genetic
Protein-Serine-Threonine Kinases
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Saccharomyces cerevisiae
description Yeast cells are able to adapt their metabolism according to the quality of both carbon and nitrogen sources available in the environment. Saccharomyces cerevisiae UGA4 gene encodes a permease capable of transporting γ-aminobutyric acid (GABA) into the cells. Yeast uses this amino acid as a nitrogen source or as a carbon skeleton that enters the tricarboxylic acid cycle.The quality of the carbon source modulates UGA4 expression through two parallel pathways, each one acting on different regulatory elements, the UAS GATA and the UAS GABA. In the presence of a fermentable carbon source, UGA4 expression is induced by GABA while in the presence of a non-fermentable carbon source this expression is GABA-independent.The aim of this work was to study the mechanisms responsible for the differences in the profiles of UGA4 expression in both growth conditions.We found that although the subcellular localization of Gln3 depends on the carbon source and UGA4 expression depends on Tor1 and Snf1, Gln3 localization does not depend on these kinases. We also found that the phosphorylation of Gln3 is mediated by two systems activated by a non-fermentable carbon source, involving the Snf1 kinase and an unidentified TORC1-regulated kinase.We also found that the activity of the main transcription factors responsible for UGA4 induction by GABA varies depending on the quality of the carbon source. In a fermentable carbon source such as glucose, the negative GATA factor Dal80 binds to UGA4 promoter; only after the addition of the inducer, the positive factors Uga3, Dal81 and Gln3 interact with the promoter removing Dal80 and leading to gene induction. In contrast, in the non-fermentable carbon source acetate the negative GATA factor remains bound to UGA4 promoter in the presence or absence of GABA, the positive factors are not detected bound in any of these conditions and in consequence, UGA4 is not induced. © 2012 Elsevier Inc.
author Cardillo, Sabrina Beatriz
Bertotti, Santiago Andrés
Correa García, Susana Raquel
author_facet Cardillo, Sabrina Beatriz
Bertotti, Santiago Andrés
Correa García, Susana Raquel
author_sort Cardillo, Sabrina Beatriz
title GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_short GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_full GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_fullStr GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_full_unstemmed GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_sort gaba induction of the saccharomyces cerevisiae uga4 gene depends on the quality of the carbon source: role of the key transcription factors acting in this process
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v421_n3_p572_Levi
http://hdl.handle.net/20.500.12110/paper_0006291X_v421_n3_p572_Levi
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AT bertottisantiagoandres gabainductionofthesaccharomycescerevisiaeuga4genedependsonthequalityofthecarbonsourceroleofthekeytranscriptionfactorsactinginthisprocess
AT correagarciasusanaraquel gabainductionofthesaccharomycescerevisiaeuga4genedependsonthequalityofthecarbonsourceroleofthekeytranscriptionfactorsactinginthisprocess
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