Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are a promising source of cells for regenerative medicine because of their potential of self renew and differentiation. Multiple evidences highlight the relationship of chromatin remodeling with stem cell properties, differentiat...

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Autores principales: Luzzani, Carlos Daniel, Losino, Noelia Ivana, Romorini, Leonardo, Barañao, José Lino S., Guberman, Alejandra Sonia
Publicado: 2011
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v410_n4_p816_Luzzani
http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n4_p816_Luzzani
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spelling paper:paper_0006291X_v410_n4_p816_Luzzani2023-06-08T14:30:19Z Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells Luzzani, Carlos Daniel Losino, Noelia Ivana Romorini, Leonardo Barañao, José Lino S. Guberman, Alejandra Sonia Chromatin modifying factors Differentiation Embryonic stem cells Induced pluripotent stem cells BMI1 protein cell protein histone deacetylase histone deacetylase 10 histone deacetylase 2 histone deacetylase 3 histone deacetylase 5 protein atf7ip protein gcn512 protein gtf3c3 protein Jarid2 protein myst2 protein suv39h2 protein taf15 unclassified drug animal cell article cell differentiation chromatin assembly and disassembly embryonic stem cell gene expression human human cell mouse nonhuman pluripotent stem cell priority journal protein expression Animals Cell Line Chromatin Chromatin Immunoprecipitation Embryonic Stem Cells Gene Expression Regulation Humans Induced Pluripotent Stem Cells Mice Transcription Factors Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are a promising source of cells for regenerative medicine because of their potential of self renew and differentiation. Multiple evidences highlight the relationship of chromatin remodeling with stem cell properties, differentiation programs and reprogramming for iPSC obtention.With the purpose of finding chromatin modifying factors relevant to these processes, and based on ChIP on chip studies, we selected several genes that could be modulated by Oct4, Sox2 and Nanog, critical transcription factors in stem cells, and studied their expression profile along the differentiation in mouse and human ESCs, and in mouse iPSCs. In this work, we analyzed the expression of Gcn5l2, GTF3C3, TAF15, ATF7IP, Myst2, HDAC2, HDAC3, HDAC5, HDAC10, SUV39H2, Jarid2, and Bmi-1. We found some genes from different functional groups that were highly modulated, suggesting that they could be relevant both in the undifferentiated state and during differentiation. These findings could contribute to the comprehension of molecular mechanisms involved in pluripotency, early differentiation and reprogramming. We believe that a deeper knowledge of the epigenetic regulation of ESC will allow improving somatic cell reprogramming for iPSC obtention and differentiation protocols optimization. © 2011 Elsevier Inc. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Losino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romorini, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v410_n4_p816_Luzzani http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n4_p816_Luzzani
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Chromatin modifying factors
Differentiation
Embryonic stem cells
Induced pluripotent stem cells
BMI1 protein
cell protein
histone deacetylase
histone deacetylase 10
histone deacetylase 2
histone deacetylase 3
histone deacetylase 5
protein atf7ip
protein gcn512
protein gtf3c3
protein Jarid2
protein myst2
protein suv39h2
protein taf15
unclassified drug
animal cell
article
cell differentiation
chromatin assembly and disassembly
embryonic stem cell
gene expression
human
human cell
mouse
nonhuman
pluripotent stem cell
priority journal
protein expression
Animals
Cell Line
Chromatin
Chromatin Immunoprecipitation
Embryonic Stem Cells
Gene Expression Regulation
Humans
Induced Pluripotent Stem Cells
Mice
Transcription Factors
spellingShingle Chromatin modifying factors
Differentiation
Embryonic stem cells
Induced pluripotent stem cells
BMI1 protein
cell protein
histone deacetylase
histone deacetylase 10
histone deacetylase 2
histone deacetylase 3
histone deacetylase 5
protein atf7ip
protein gcn512
protein gtf3c3
protein Jarid2
protein myst2
protein suv39h2
protein taf15
unclassified drug
animal cell
article
cell differentiation
chromatin assembly and disassembly
embryonic stem cell
gene expression
human
human cell
mouse
nonhuman
pluripotent stem cell
priority journal
protein expression
Animals
Cell Line
Chromatin
Chromatin Immunoprecipitation
Embryonic Stem Cells
Gene Expression Regulation
Humans
Induced Pluripotent Stem Cells
Mice
Transcription Factors
Luzzani, Carlos Daniel
Losino, Noelia Ivana
Romorini, Leonardo
Barañao, José Lino S.
Guberman, Alejandra Sonia
Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
topic_facet Chromatin modifying factors
Differentiation
Embryonic stem cells
Induced pluripotent stem cells
BMI1 protein
cell protein
histone deacetylase
histone deacetylase 10
histone deacetylase 2
histone deacetylase 3
histone deacetylase 5
protein atf7ip
protein gcn512
protein gtf3c3
protein Jarid2
protein myst2
protein suv39h2
protein taf15
unclassified drug
animal cell
article
cell differentiation
chromatin assembly and disassembly
embryonic stem cell
gene expression
human
human cell
mouse
nonhuman
pluripotent stem cell
priority journal
protein expression
Animals
Cell Line
Chromatin
Chromatin Immunoprecipitation
Embryonic Stem Cells
Gene Expression Regulation
Humans
Induced Pluripotent Stem Cells
Mice
Transcription Factors
description Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are a promising source of cells for regenerative medicine because of their potential of self renew and differentiation. Multiple evidences highlight the relationship of chromatin remodeling with stem cell properties, differentiation programs and reprogramming for iPSC obtention.With the purpose of finding chromatin modifying factors relevant to these processes, and based on ChIP on chip studies, we selected several genes that could be modulated by Oct4, Sox2 and Nanog, critical transcription factors in stem cells, and studied their expression profile along the differentiation in mouse and human ESCs, and in mouse iPSCs. In this work, we analyzed the expression of Gcn5l2, GTF3C3, TAF15, ATF7IP, Myst2, HDAC2, HDAC3, HDAC5, HDAC10, SUV39H2, Jarid2, and Bmi-1. We found some genes from different functional groups that were highly modulated, suggesting that they could be relevant both in the undifferentiated state and during differentiation. These findings could contribute to the comprehension of molecular mechanisms involved in pluripotency, early differentiation and reprogramming. We believe that a deeper knowledge of the epigenetic regulation of ESC will allow improving somatic cell reprogramming for iPSC obtention and differentiation protocols optimization. © 2011 Elsevier Inc.
author Luzzani, Carlos Daniel
Losino, Noelia Ivana
Romorini, Leonardo
Barañao, José Lino S.
Guberman, Alejandra Sonia
author_facet Luzzani, Carlos Daniel
Losino, Noelia Ivana
Romorini, Leonardo
Barañao, José Lino S.
Guberman, Alejandra Sonia
author_sort Luzzani, Carlos Daniel
title Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
title_short Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
title_full Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
title_fullStr Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
title_full_unstemmed Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
title_sort modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
publishDate 2011
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v410_n4_p816_Luzzani
http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n4_p816_Luzzani
work_keys_str_mv AT luzzanicarlosdaniel modulationofchromatinmodifyingfactorsgeneexpressioninembryonicandinducedpluripotentstemcells
AT losinonoeliaivana modulationofchromatinmodifyingfactorsgeneexpressioninembryonicandinducedpluripotentstemcells
AT romorinileonardo modulationofchromatinmodifyingfactorsgeneexpressioninembryonicandinducedpluripotentstemcells
AT baranaojoselinos modulationofchromatinmodifyingfactorsgeneexpressioninembryonicandinducedpluripotentstemcells
AT gubermanalejandrasonia modulationofchromatinmodifyingfactorsgeneexpressioninembryonicandinducedpluripotentstemcells
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