Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication
Leukaemia inhibitory factor (LIF) or Oncostatin M (OSM), both mitogens for Swiss mouse 3T3 cells, triggers initiation of DNA synthesis without the requirement for mevalonic acid. Thus, Lovastatin (LOV), an inhibitor of the hydroxy methylglutaryl CoA (HMGCoA) reductase, does not block LIF or OSM indu...
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2004
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v313_n4_p926_Sauane http://hdl.handle.net/20.500.12110/paper_0006291X_v313_n4_p926_Sauane |
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paper:paper_0006291X_v313_n4_p926_Sauane2023-06-08T14:30:14Z Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication DNA replication Insulin Leukaemia inhibitory factor Mevalonic acid Oncostatin M 3 hydroxy 3 methylglutaryl coenzyme A DNA leukemia inhibitory factor mevalonic acid mevinolin oncostatin M animal cell article cell cycle S phase cell division cell strain 3T3 controlled study DNA replication leukemia nonhuman priority journal protein induction protein processing signal transduction Leukaemia inhibitory factor (LIF) or Oncostatin M (OSM), both mitogens for Swiss mouse 3T3 cells, triggers initiation of DNA synthesis without the requirement for mevalonic acid. Thus, Lovastatin (LOV), an inhibitor of the hydroxy methylglutaryl CoA (HMGCoA) reductase, does not block LIF or OSM induced DNA replication and cell multiplication. In contrast, increasing concentrations of LOV from 1 to 60 μM block the mitogenic action of PGF 2α by decreasing the number of cells capable of entering S-phase and dividing. This inhibition by LOV can be reversed by addition of mevanolactone (MEV), an analogue of mevalonic acid. Thus, LIF or OSM triggers initiation of DNA replication independently of mevalonic acid synthesis and therefore without the involvement of isoprenylation of various signalling proteins. © 2003 Elsevier Inc. All rights reserved. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v313_n4_p926_Sauane http://hdl.handle.net/20.500.12110/paper_0006291X_v313_n4_p926_Sauane |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
DNA replication Insulin Leukaemia inhibitory factor Mevalonic acid Oncostatin M 3 hydroxy 3 methylglutaryl coenzyme A DNA leukemia inhibitory factor mevalonic acid mevinolin oncostatin M animal cell article cell cycle S phase cell division cell strain 3T3 controlled study DNA replication leukemia nonhuman priority journal protein induction protein processing signal transduction |
spellingShingle |
DNA replication Insulin Leukaemia inhibitory factor Mevalonic acid Oncostatin M 3 hydroxy 3 methylglutaryl coenzyme A DNA leukemia inhibitory factor mevalonic acid mevinolin oncostatin M animal cell article cell cycle S phase cell division cell strain 3T3 controlled study DNA replication leukemia nonhuman priority journal protein induction protein processing signal transduction Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication |
topic_facet |
DNA replication Insulin Leukaemia inhibitory factor Mevalonic acid Oncostatin M 3 hydroxy 3 methylglutaryl coenzyme A DNA leukemia inhibitory factor mevalonic acid mevinolin oncostatin M animal cell article cell cycle S phase cell division cell strain 3T3 controlled study DNA replication leukemia nonhuman priority journal protein induction protein processing signal transduction |
description |
Leukaemia inhibitory factor (LIF) or Oncostatin M (OSM), both mitogens for Swiss mouse 3T3 cells, triggers initiation of DNA synthesis without the requirement for mevalonic acid. Thus, Lovastatin (LOV), an inhibitor of the hydroxy methylglutaryl CoA (HMGCoA) reductase, does not block LIF or OSM induced DNA replication and cell multiplication. In contrast, increasing concentrations of LOV from 1 to 60 μM block the mitogenic action of PGF 2α by decreasing the number of cells capable of entering S-phase and dividing. This inhibition by LOV can be reversed by addition of mevanolactone (MEV), an analogue of mevalonic acid. Thus, LIF or OSM triggers initiation of DNA replication independently of mevalonic acid synthesis and therefore without the involvement of isoprenylation of various signalling proteins. © 2003 Elsevier Inc. All rights reserved. |
title |
Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication |
title_short |
Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication |
title_full |
Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication |
title_fullStr |
Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication |
title_full_unstemmed |
Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication |
title_sort |
leukaemia inhibitory factor or oncostatin m induction of swiss 3t3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate dna replication |
publishDate |
2004 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v313_n4_p926_Sauane http://hdl.handle.net/20.500.12110/paper_0006291X_v313_n4_p926_Sauane |
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1768546048944373760 |