Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a l...
Guardado en:
Autor principal: | |
---|---|
Publicado: |
2000
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v272_n3_p946_Moretti http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti |
Aporte de: |
id |
paper:paper_0006291X_v272_n3_p946_Moretti |
---|---|
record_format |
dspace |
spelling |
paper:paper_0006291X_v272_n3_p946_Moretti2023-06-08T14:30:11Z Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae Batlle, Alcira María del Carmen Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a limiting step for its intracellular metabolization. To determine the relationship between ALA transport and ALA metabolization, ALA incorporation was measured in yeast mutant strains deficient in the δ-aminolevulinic acid-synthase, uroporphyrinogen III decarboxylase, and ferrochelatase, three enzymes involved in porphyrin biosynthesis. Results presented here showed that neither intracellular ALA nor uroporphyrin or protoporphyrin regulates ALA incorporation, indicating that ALA uptake and its subsequent metabolization are not related to each other. Thus a key metabolite as it is, ALA does not have a transport system regulated according to its role. (C) 2000 Academic Press. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v272_n3_p946_Moretti http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae |
spellingShingle |
Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae Batlle, Alcira María del Carmen Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae |
topic_facet |
Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae |
description |
In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a limiting step for its intracellular metabolization. To determine the relationship between ALA transport and ALA metabolization, ALA incorporation was measured in yeast mutant strains deficient in the δ-aminolevulinic acid-synthase, uroporphyrinogen III decarboxylase, and ferrochelatase, three enzymes involved in porphyrin biosynthesis. Results presented here showed that neither intracellular ALA nor uroporphyrin or protoporphyrin regulates ALA incorporation, indicating that ALA uptake and its subsequent metabolization are not related to each other. Thus a key metabolite as it is, ALA does not have a transport system regulated according to its role. (C) 2000 Academic Press. |
author |
Batlle, Alcira María del Carmen |
author_facet |
Batlle, Alcira María del Carmen |
author_sort |
Batlle, Alcira María del Carmen |
title |
Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae |
title_short |
Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae |
title_full |
Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae |
title_fullStr |
Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae |
title_full_unstemmed |
Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae |
title_sort |
porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in saccharomyces cerevisiae |
publishDate |
2000 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v272_n3_p946_Moretti http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti |
work_keys_str_mv |
AT batllealciramariadelcarmen porphyrinbiosynthesisintermediatesarenotregulatingdaminolevulinicacidtransportinsaccharomycescerevisiae |
_version_ |
1768545906498469888 |