Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae

In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a l...

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Autor principal: Batlle, Alcira María del Carmen
Publicado: 2000
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v272_n3_p946_Moretti
http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti
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spelling paper:paper_0006291X_v272_n3_p946_Moretti2023-06-08T14:30:11Z Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae Batlle, Alcira María del Carmen Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a limiting step for its intracellular metabolization. To determine the relationship between ALA transport and ALA metabolization, ALA incorporation was measured in yeast mutant strains deficient in the δ-aminolevulinic acid-synthase, uroporphyrinogen III decarboxylase, and ferrochelatase, three enzymes involved in porphyrin biosynthesis. Results presented here showed that neither intracellular ALA nor uroporphyrin or protoporphyrin regulates ALA incorporation, indicating that ALA uptake and its subsequent metabolization are not related to each other. Thus a key metabolite as it is, ALA does not have a transport system regulated according to its role. (C) 2000 Academic Press. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v272_n3_p946_Moretti http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Membrane transport
Porphyrin biosynthesis
Saccharomyces cerevisiae
Transport regulation
Uga4 permease
γ-aminobutyric acid
δ-aminolevulinic acid
4 aminobutyric acid
5 aminolevulinate synthase
aminolevulinic acid
ferrochelatase
permease
porphyrin
uroporphyrinogen decarboxylase
article
cell membrane transport
cell mutant
controlled study
enzyme deficiency
metabolism
nonhuman
priority journal
regulatory mechanism
Saccharomyces cerevisiae
Eukaryota
Saccharomyces cerevisiae
Saccharomyces cerevisiae
spellingShingle Membrane transport
Porphyrin biosynthesis
Saccharomyces cerevisiae
Transport regulation
Uga4 permease
γ-aminobutyric acid
δ-aminolevulinic acid
4 aminobutyric acid
5 aminolevulinate synthase
aminolevulinic acid
ferrochelatase
permease
porphyrin
uroporphyrinogen decarboxylase
article
cell membrane transport
cell mutant
controlled study
enzyme deficiency
metabolism
nonhuman
priority journal
regulatory mechanism
Saccharomyces cerevisiae
Eukaryota
Saccharomyces cerevisiae
Saccharomyces cerevisiae
Batlle, Alcira María del Carmen
Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
topic_facet Membrane transport
Porphyrin biosynthesis
Saccharomyces cerevisiae
Transport regulation
Uga4 permease
γ-aminobutyric acid
δ-aminolevulinic acid
4 aminobutyric acid
5 aminolevulinate synthase
aminolevulinic acid
ferrochelatase
permease
porphyrin
uroporphyrinogen decarboxylase
article
cell membrane transport
cell mutant
controlled study
enzyme deficiency
metabolism
nonhuman
priority journal
regulatory mechanism
Saccharomyces cerevisiae
Eukaryota
Saccharomyces cerevisiae
Saccharomyces cerevisiae
description In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a limiting step for its intracellular metabolization. To determine the relationship between ALA transport and ALA metabolization, ALA incorporation was measured in yeast mutant strains deficient in the δ-aminolevulinic acid-synthase, uroporphyrinogen III decarboxylase, and ferrochelatase, three enzymes involved in porphyrin biosynthesis. Results presented here showed that neither intracellular ALA nor uroporphyrin or protoporphyrin regulates ALA incorporation, indicating that ALA uptake and its subsequent metabolization are not related to each other. Thus a key metabolite as it is, ALA does not have a transport system regulated according to its role. (C) 2000 Academic Press.
author Batlle, Alcira María del Carmen
author_facet Batlle, Alcira María del Carmen
author_sort Batlle, Alcira María del Carmen
title Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_short Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_full Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_fullStr Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_full_unstemmed Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_sort porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in saccharomyces cerevisiae
publishDate 2000
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v272_n3_p946_Moretti
http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti
work_keys_str_mv AT batllealciramariadelcarmen porphyrinbiosynthesisintermediatesarenotregulatingdaminolevulinicacidtransportinsaccharomycescerevisiae
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