Bradykinin stimulates phospholipase C in rat renal medullary slices

Rat renal medullary slices prelabeled with [14C]arachidonic acid generate [14C]diacylglycerol within 1 min of exposure to bradykinin action. Production of [14C]diacylglycerol is transient. 2 min after the addition of bradykinin, the levels of metabolite reach the maximun, but decrease thereafter. Si...

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Publicado:	1985
Materias:	(Rat kidney medulla) Bradykinin Diacylglycerol Phosphatidylinositol Phospholipase C bradykinin bucladesine enzyme indometacin mepacrine phosphatidylinositol phospholipase c radioisotope animal cell arachidonic acid c 14 diacylglycerol c 14 dose response drug comparison drug elimination drug inhibition drug mechanism drug metabolism drug monitoring drug response kidney kidney medulla nonhuman phosphatidylinositol c 14 priority journal rat thin layer chromatography Animal Bucladesine Diglycerides Enzyme Activation Indomethacin Kidney Medulla Male Phosphatidylinositols Phospholipases Phospholipids Quinacrine Rats Rats, Inbred Strains Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v836_n1_p14_Speziale http://hdl.handle.net/20.500.12110/paper_00052760_v836_n1_p14_Speziale
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00052760_v836_n1_p14_Speziale
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spelling	paper:paper_00052760_v836_n1_p14_Speziale2023-06-08T14:29:57Z Bradykinin stimulates phospholipase C in rat renal medullary slices (Rat kidney medulla) Bradykinin Diacylglycerol Phosphatidylinositol Phospholipase C bradykinin bucladesine enzyme indometacin mepacrine phosphatidylinositol phospholipase c radioisotope animal cell arachidonic acid c 14 diacylglycerol c 14 dose response drug comparison drug elimination drug inhibition drug mechanism drug metabolism drug monitoring drug response kidney kidney medulla nonhuman phosphatidylinositol c 14 priority journal rat thin layer chromatography Animal Bradykinin Bucladesine Diglycerides Enzyme Activation Indomethacin Kidney Medulla Male Phosphatidylinositols Phospholipase C Phospholipases Phospholipids Quinacrine Rats Rats, Inbred Strains Support, Non-U.S. Gov't Rat renal medullary slices prelabeled with [14C]arachidonic acid generate [14C]diacylglycerol within 1 min of exposure to bradykinin action. Production of [14C]diacylglycerol is transient. 2 min after the addition of bradykinin, the levels of metabolite reach the maximun, but decrease thereafter. Simultaneously, bradykinin induces a parallel decrease of the radioactivity in phosphatidylinositol. No degradation of other phospholipids is observed, and triacylglycerol is not affected. The degradation of [14C]phosphatidylinositol to (14C]diacylglycerol indicated the presence of phospholipase C acivity. Preincubation of prelabeled slices with 2 mM dibutyryl cyclic AMP prevents both the generation of diacylglycerol and the degradation of phosphatidylinositol. Neither mepacrine nor indomethacin block diacylglycerol production and phosphatidylinositol breakdown. We conclude that, when rat renal medullary slices are stimulated with bradykinin, phosphatidylinositol-specific phospholipase C is activated. © 1985. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v836_n1_p14_Speziale http://hdl.handle.net/20.500.12110/paper_00052760_v836_n1_p14_Speziale
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	(Rat kidney medulla) Bradykinin Diacylglycerol Phosphatidylinositol Phospholipase C bradykinin bucladesine enzyme indometacin mepacrine phosphatidylinositol phospholipase c radioisotope animal cell arachidonic acid c 14 diacylglycerol c 14 dose response drug comparison drug elimination drug inhibition drug mechanism drug metabolism drug monitoring drug response kidney kidney medulla nonhuman phosphatidylinositol c 14 priority journal rat thin layer chromatography Animal Bradykinin Bucladesine Diglycerides Enzyme Activation Indomethacin Kidney Medulla Male Phosphatidylinositols Phospholipase C Phospholipases Phospholipids Quinacrine Rats Rats, Inbred Strains Support, Non-U.S. Gov't
spellingShingle	(Rat kidney medulla) Bradykinin Diacylglycerol Phosphatidylinositol Phospholipase C bradykinin bucladesine enzyme indometacin mepacrine phosphatidylinositol phospholipase c radioisotope animal cell arachidonic acid c 14 diacylglycerol c 14 dose response drug comparison drug elimination drug inhibition drug mechanism drug metabolism drug monitoring drug response kidney kidney medulla nonhuman phosphatidylinositol c 14 priority journal rat thin layer chromatography Animal Bradykinin Bucladesine Diglycerides Enzyme Activation Indomethacin Kidney Medulla Male Phosphatidylinositols Phospholipase C Phospholipases Phospholipids Quinacrine Rats Rats, Inbred Strains Support, Non-U.S. Gov't Bradykinin stimulates phospholipase C in rat renal medullary slices
topic_facet	(Rat kidney medulla) Bradykinin Diacylglycerol Phosphatidylinositol Phospholipase C bradykinin bucladesine enzyme indometacin mepacrine phosphatidylinositol phospholipase c radioisotope animal cell arachidonic acid c 14 diacylglycerol c 14 dose response drug comparison drug elimination drug inhibition drug mechanism drug metabolism drug monitoring drug response kidney kidney medulla nonhuman phosphatidylinositol c 14 priority journal rat thin layer chromatography Animal Bradykinin Bucladesine Diglycerides Enzyme Activation Indomethacin Kidney Medulla Male Phosphatidylinositols Phospholipase C Phospholipases Phospholipids Quinacrine Rats Rats, Inbred Strains Support, Non-U.S. Gov't
description	Rat renal medullary slices prelabeled with [14C]arachidonic acid generate [14C]diacylglycerol within 1 min of exposure to bradykinin action. Production of [14C]diacylglycerol is transient. 2 min after the addition of bradykinin, the levels of metabolite reach the maximun, but decrease thereafter. Simultaneously, bradykinin induces a parallel decrease of the radioactivity in phosphatidylinositol. No degradation of other phospholipids is observed, and triacylglycerol is not affected. The degradation of [14C]phosphatidylinositol to (14C]diacylglycerol indicated the presence of phospholipase C acivity. Preincubation of prelabeled slices with 2 mM dibutyryl cyclic AMP prevents both the generation of diacylglycerol and the degradation of phosphatidylinositol. Neither mepacrine nor indomethacin block diacylglycerol production and phosphatidylinositol breakdown. We conclude that, when rat renal medullary slices are stimulated with bradykinin, phosphatidylinositol-specific phospholipase C is activated. © 1985.
title	Bradykinin stimulates phospholipase C in rat renal medullary slices
title_short	Bradykinin stimulates phospholipase C in rat renal medullary slices
title_full	Bradykinin stimulates phospholipase C in rat renal medullary slices
title_fullStr	Bradykinin stimulates phospholipase C in rat renal medullary slices
title_full_unstemmed	Bradykinin stimulates phospholipase C in rat renal medullary slices
title_sort	bradykinin stimulates phospholipase c in rat renal medullary slices
publishDate	1985
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052760_v836_n1_p14_Speziale http://hdl.handle.net/20.500.12110/paper_00052760_v836_n1_p14_Speziale
_version_	1768544847912763392

Bradykinin stimulates phospholipase C in rat renal medullary slices

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