Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties

1. 1. Uroporphyrinogen carboxy-lyase (EC 4.1.1.d), the enzyme catalysing the decaroxylation of uroporphyrinogen to coproporphyrinogen, has been isolated from normal chicken erythrocytes. The enzyme was purified 220-fold with a yield of 24% from haemolysate supernatant by DEAE-cellulose batch treatme...

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Autores principales:	Tomio, Josefina María, García, Rodolfo Carlos, San Martín de Viale, Leonor Carmen, Grinstein, Moisés
Publicado:	1970
Materias:	acrylic acid derivative ammonium derivative Carboxy Lyases carboxylyase cellulose edetic acid glutathione porphyrin sodium sulfate air animal article chicken drug stability enzyme activation enzymology erythrocyte gel gel electrophoresis heat ion exchange chromatography isolation and purification liver pH precipitation rat technique Acrylates Air Ammonium Compounds Animal Carboxy-Lyases Cellulose Chickens Chromatography, DEAE-Cellulose Drug Stability Edetic Acid Electrophoresis, Disc Enzyme Activation Erythrocytes Gels Glutathione Heat Hydrogen-Ion Concentration Liver Methods Porphyrins Precipitation Rats Sodium Sulfates
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v198_n2_p353_Tomio http://hdl.handle.net/20.500.12110/paper_00052744_v198_n2_p353_Tomio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00052744_v198_n2_p353_Tomio2023-06-08T14:29:53Z Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties Tomio, Josefina María García, Rodolfo Carlos San Martín de Viale, Leonor Carmen Grinstein, Moisés acrylic acid derivative ammonium derivative Carboxy Lyases carboxylyase cellulose edetic acid glutathione porphyrin sodium sulfate air animal article chicken drug stability enzyme activation enzymology erythrocyte gel gel electrophoresis heat ion exchange chromatography isolation and purification liver pH precipitation rat technique Acrylates Air Ammonium Compounds Animal Carboxy-Lyases Cellulose Chickens Chromatography, DEAE-Cellulose Drug Stability Edetic Acid Electrophoresis, Disc Enzyme Activation Erythrocytes Gels Glutathione Heat Hydrogen-Ion Concentration Liver Methods Porphyrins Precipitation Rats Sodium Sulfates 1. 1. Uroporphyrinogen carboxy-lyase (EC 4.1.1.d), the enzyme catalysing the decaroxylation of uroporphyrinogen to coproporphyrinogen, has been isolated from normal chicken erythrocytes. The enzyme was purified 220-fold with a yield of 24% from haemolysate supernatant by DEAE-cellulose batch treatment, (NH4)2SO4 fractionation and chromatography on DEAE-cellulose. 2. 2. The purified material appears to be homogeneous in polyacrylamide gel disc electrophoresis. 3. 3. The enzyme was heat labile and inhibited by sodium salt; the activity was enhanced by EDTA, GSH and boiled rat-liver extract. 4. 4. The influence of these chemical and physical agents on the removal of the first and second carboxyl groups from uroporphyrinogen was compared; the second group was more susceptible to these agents. 5. 5. The possibility that one or several enzymes were involved in the stepwise decarboxylation of uroporphyrinogen is discussed. 6. 6. The general name of porphyrinogen carboxy-lyase for the enzyme system is proposed because of the different porphyrinogens it can decarboxylate. © 1970. Fil:Tomio, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:García, R.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:San Martín De Viale, L.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1970 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v198_n2_p353_Tomio http://hdl.handle.net/20.500.12110/paper_00052744_v198_n2_p353_Tomio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acrylic acid derivative ammonium derivative Carboxy Lyases carboxylyase cellulose edetic acid glutathione porphyrin sodium sulfate air animal article chicken drug stability enzyme activation enzymology erythrocyte gel gel electrophoresis heat ion exchange chromatography isolation and purification liver pH precipitation rat technique Acrylates Air Ammonium Compounds Animal Carboxy-Lyases Cellulose Chickens Chromatography, DEAE-Cellulose Drug Stability Edetic Acid Electrophoresis, Disc Enzyme Activation Erythrocytes Gels Glutathione Heat Hydrogen-Ion Concentration Liver Methods Porphyrins Precipitation Rats Sodium Sulfates
spellingShingle	acrylic acid derivative ammonium derivative Carboxy Lyases carboxylyase cellulose edetic acid glutathione porphyrin sodium sulfate air animal article chicken drug stability enzyme activation enzymology erythrocyte gel gel electrophoresis heat ion exchange chromatography isolation and purification liver pH precipitation rat technique Acrylates Air Ammonium Compounds Animal Carboxy-Lyases Cellulose Chickens Chromatography, DEAE-Cellulose Drug Stability Edetic Acid Electrophoresis, Disc Enzyme Activation Erythrocytes Gels Glutathione Heat Hydrogen-Ion Concentration Liver Methods Porphyrins Precipitation Rats Sodium Sulfates Tomio, Josefina María García, Rodolfo Carlos San Martín de Viale, Leonor Carmen Grinstein, Moisés Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties
topic_facet	acrylic acid derivative ammonium derivative Carboxy Lyases carboxylyase cellulose edetic acid glutathione porphyrin sodium sulfate air animal article chicken drug stability enzyme activation enzymology erythrocyte gel gel electrophoresis heat ion exchange chromatography isolation and purification liver pH precipitation rat technique Acrylates Air Ammonium Compounds Animal Carboxy-Lyases Cellulose Chickens Chromatography, DEAE-Cellulose Drug Stability Edetic Acid Electrophoresis, Disc Enzyme Activation Erythrocytes Gels Glutathione Heat Hydrogen-Ion Concentration Liver Methods Porphyrins Precipitation Rats Sodium Sulfates
description	1. 1. Uroporphyrinogen carboxy-lyase (EC 4.1.1.d), the enzyme catalysing the decaroxylation of uroporphyrinogen to coproporphyrinogen, has been isolated from normal chicken erythrocytes. The enzyme was purified 220-fold with a yield of 24% from haemolysate supernatant by DEAE-cellulose batch treatment, (NH4)2SO4 fractionation and chromatography on DEAE-cellulose. 2. 2. The purified material appears to be homogeneous in polyacrylamide gel disc electrophoresis. 3. 3. The enzyme was heat labile and inhibited by sodium salt; the activity was enhanced by EDTA, GSH and boiled rat-liver extract. 4. 4. The influence of these chemical and physical agents on the removal of the first and second carboxyl groups from uroporphyrinogen was compared; the second group was more susceptible to these agents. 5. 5. The possibility that one or several enzymes were involved in the stepwise decarboxylation of uroporphyrinogen is discussed. 6. 6. The general name of porphyrinogen carboxy-lyase for the enzyme system is proposed because of the different porphyrinogens it can decarboxylate. © 1970.
author	Tomio, Josefina María García, Rodolfo Carlos San Martín de Viale, Leonor Carmen Grinstein, Moisés
author_facet	Tomio, Josefina María García, Rodolfo Carlos San Martín de Viale, Leonor Carmen Grinstein, Moisés
author_sort	Tomio, Josefina María
title	Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties
title_short	Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties
title_full	Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties
title_fullStr	Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties
title_full_unstemmed	Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties
title_sort	porphyrin biosynthesis. vii. porphyrinogen carboxy-lyase from avian erythrocytes. purification and properties
publishDate	1970
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v198_n2_p353_Tomio http://hdl.handle.net/20.500.12110/paper_00052744_v198_n2_p353_Tomio
work_keys_str_mv	AT tomiojosefinamaria porphyrinbiosynthesisviiporphyrinogencarboxylyasefromavianerythrocytespurificationandproperties AT garciarodolfocarlos porphyrinbiosynthesisviiporphyrinogencarboxylyasefromavianerythrocytespurificationandproperties AT sanmartindevialeleonorcarmen porphyrinbiosynthesisviiporphyrinogencarboxylyasefromavianerythrocytespurificationandproperties AT grinsteinmoises porphyrinbiosynthesisviiporphyrinogencarboxylyasefromavianerythrocytespurificationandproperties
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Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties

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