Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues
Nanoparticles (NPs) of diamond, titanium dioxide, titanium silicon oxide, barium strontium titanium oxide, and silver (Ag) were examined for their potential as MALDI matrixes for direct laser desorption/ionization of carbohydrates, especially fructans, from plant tissue. Two sample preparation metho...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v82_n13_p5518_Gholipour http://hdl.handle.net/20.500.12110/paper_00032700_v82_n13_p5518_Gholipour |
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paper:paper_00032700_v82_n13_p5518_Gholipour2023-06-08T14:24:12Z Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues Barium strontium titanium oxide Dihydroxybenzoic acids Doped sample Fructans Harmane In-plants In-vivo Kestose Laser desorption/ionization Limit of detection MALDI Matrix matrix Matrix-assisted laser desorption/ionization mass spectrometry Molecular ions Plant tissues Positive ion mode Reproducibilities Sample preparation methods Signal acquisitions Signal intensities Soluble sugars Solvent free Barium Barium compounds Carbon nanotubes Desorption Histology Mass spectrometry Nanoparticles Negative ions Organic compounds Polysaccharides Signal processing Signal to noise ratio Silicon compounds Silicon oxides Silver Silver oxides Solvents Strontium Sugar (sucrose) Tissue Titanium Titanium dioxide Titanium oxides 1 kestose 1-kestose barium carbon nanotube diamond fructan gold nanoparticle oxide silicon dioxide strontium strontium titanium oxide sucrose titanium titanium dioxide trisaccharide diamond fructan nanoparticle oxide silicon dioxide strontium titanium article chemistry mass spectrometry methodology plant root tulip mass spectrometry procedures Barium Diamond Fructans Gold Nanoparticles Nanotubes, Carbon Oxides Plant Roots Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strontium Sucrose Titanium Trisaccharides Tulipa Barium Diamond Fructans Gold Nanoparticles Nanotubes, Carbon Oxides Plant Roots Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strontium Sucrose Titanium Trisaccharides Tulipa Nanoparticles (NPs) of diamond, titanium dioxide, titanium silicon oxide, barium strontium titanium oxide, and silver (Ag) were examined for their potential as MALDI matrixes for direct laser desorption/ionization of carbohydrates, especially fructans, from plant tissue. Two sample preparation methods including solvent-assisted and solvent-free (dry) NPs deposition were performed and compared. All examined NPs except for Ag could desorb/ionize standard sucrose and fructans in positive and in negative ion mode. Ag NPs yielded good signals only for nonsalt-doped samples that were measured in the negative ion mode. In the case of in vivo studies, except for Ag, all NPs studied could desorb/ionize carbohydrates from tissue in both the positive and negative ion modes. Furthermore, compared to the results obtained with soluble sugars extracted from plant tissues, fructans with higher molecular weight intact molecular ions could be detected when the plant tissues were directly profiled. The limit of detection (LOD) of fructans and the ratios between signal intensities and fructan concentrations were analyzed. NPs had similar LODs for standard fructan triose (1-kestose) in the positive ion mode and better LODs in the negative ion mode when compared with the common crystalline organic MALDI matrixes used for carbohydrates (2,5-dihydroxybenzoic acid and nor-harmane) or carbon nanotubes. Solvent-free NP deposition on tissues partially improves the signal acquisition. Although lower signal-to-noise ratio sugar signals were acquired from the tissues when compared to the solvent-assisted method, the reproducibility averaged over all sample was more uniform. © 2010 American Chemical Society. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v82_n13_p5518_Gholipour http://hdl.handle.net/20.500.12110/paper_00032700_v82_n13_p5518_Gholipour |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Barium strontium titanium oxide Dihydroxybenzoic acids Doped sample Fructans Harmane In-plants In-vivo Kestose Laser desorption/ionization Limit of detection MALDI Matrix matrix Matrix-assisted laser desorption/ionization mass spectrometry Molecular ions Plant tissues Positive ion mode Reproducibilities Sample preparation methods Signal acquisitions Signal intensities Soluble sugars Solvent free Barium Barium compounds Carbon nanotubes Desorption Histology Mass spectrometry Nanoparticles Negative ions Organic compounds Polysaccharides Signal processing Signal to noise ratio Silicon compounds Silicon oxides Silver Silver oxides Solvents Strontium Sugar (sucrose) Tissue Titanium Titanium dioxide Titanium oxides 1 kestose 1-kestose barium carbon nanotube diamond fructan gold nanoparticle oxide silicon dioxide strontium strontium titanium oxide sucrose titanium titanium dioxide trisaccharide diamond fructan nanoparticle oxide silicon dioxide strontium titanium article chemistry mass spectrometry methodology plant root tulip mass spectrometry procedures Barium Diamond Fructans Gold Nanoparticles Nanotubes, Carbon Oxides Plant Roots Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strontium Sucrose Titanium Trisaccharides Tulipa Barium Diamond Fructans Gold Nanoparticles Nanotubes, Carbon Oxides Plant Roots Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strontium Sucrose Titanium Trisaccharides Tulipa |
| spellingShingle |
Barium strontium titanium oxide Dihydroxybenzoic acids Doped sample Fructans Harmane In-plants In-vivo Kestose Laser desorption/ionization Limit of detection MALDI Matrix matrix Matrix-assisted laser desorption/ionization mass spectrometry Molecular ions Plant tissues Positive ion mode Reproducibilities Sample preparation methods Signal acquisitions Signal intensities Soluble sugars Solvent free Barium Barium compounds Carbon nanotubes Desorption Histology Mass spectrometry Nanoparticles Negative ions Organic compounds Polysaccharides Signal processing Signal to noise ratio Silicon compounds Silicon oxides Silver Silver oxides Solvents Strontium Sugar (sucrose) Tissue Titanium Titanium dioxide Titanium oxides 1 kestose 1-kestose barium carbon nanotube diamond fructan gold nanoparticle oxide silicon dioxide strontium strontium titanium oxide sucrose titanium titanium dioxide trisaccharide diamond fructan nanoparticle oxide silicon dioxide strontium titanium article chemistry mass spectrometry methodology plant root tulip mass spectrometry procedures Barium Diamond Fructans Gold Nanoparticles Nanotubes, Carbon Oxides Plant Roots Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strontium Sucrose Titanium Trisaccharides Tulipa Barium Diamond Fructans Gold Nanoparticles Nanotubes, Carbon Oxides Plant Roots Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strontium Sucrose Titanium Trisaccharides Tulipa Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues |
| topic_facet |
Barium strontium titanium oxide Dihydroxybenzoic acids Doped sample Fructans Harmane In-plants In-vivo Kestose Laser desorption/ionization Limit of detection MALDI Matrix matrix Matrix-assisted laser desorption/ionization mass spectrometry Molecular ions Plant tissues Positive ion mode Reproducibilities Sample preparation methods Signal acquisitions Signal intensities Soluble sugars Solvent free Barium Barium compounds Carbon nanotubes Desorption Histology Mass spectrometry Nanoparticles Negative ions Organic compounds Polysaccharides Signal processing Signal to noise ratio Silicon compounds Silicon oxides Silver Silver oxides Solvents Strontium Sugar (sucrose) Tissue Titanium Titanium dioxide Titanium oxides 1 kestose 1-kestose barium carbon nanotube diamond fructan gold nanoparticle oxide silicon dioxide strontium strontium titanium oxide sucrose titanium titanium dioxide trisaccharide diamond fructan nanoparticle oxide silicon dioxide strontium titanium article chemistry mass spectrometry methodology plant root tulip mass spectrometry procedures Barium Diamond Fructans Gold Nanoparticles Nanotubes, Carbon Oxides Plant Roots Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strontium Sucrose Titanium Trisaccharides Tulipa Barium Diamond Fructans Gold Nanoparticles Nanotubes, Carbon Oxides Plant Roots Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strontium Sucrose Titanium Trisaccharides Tulipa |
| description |
Nanoparticles (NPs) of diamond, titanium dioxide, titanium silicon oxide, barium strontium titanium oxide, and silver (Ag) were examined for their potential as MALDI matrixes for direct laser desorption/ionization of carbohydrates, especially fructans, from plant tissue. Two sample preparation methods including solvent-assisted and solvent-free (dry) NPs deposition were performed and compared. All examined NPs except for Ag could desorb/ionize standard sucrose and fructans in positive and in negative ion mode. Ag NPs yielded good signals only for nonsalt-doped samples that were measured in the negative ion mode. In the case of in vivo studies, except for Ag, all NPs studied could desorb/ionize carbohydrates from tissue in both the positive and negative ion modes. Furthermore, compared to the results obtained with soluble sugars extracted from plant tissues, fructans with higher molecular weight intact molecular ions could be detected when the plant tissues were directly profiled. The limit of detection (LOD) of fructans and the ratios between signal intensities and fructan concentrations were analyzed. NPs had similar LODs for standard fructan triose (1-kestose) in the positive ion mode and better LODs in the negative ion mode when compared with the common crystalline organic MALDI matrixes used for carbohydrates (2,5-dihydroxybenzoic acid and nor-harmane) or carbon nanotubes. Solvent-free NP deposition on tissues partially improves the signal acquisition. Although lower signal-to-noise ratio sugar signals were acquired from the tissues when compared to the solvent-assisted method, the reproducibility averaged over all sample was more uniform. © 2010 American Chemical Society. |
| title |
Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues |
| title_short |
Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues |
| title_full |
Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues |
| title_fullStr |
Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues |
| title_full_unstemmed |
Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues |
| title_sort |
diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v82_n13_p5518_Gholipour http://hdl.handle.net/20.500.12110/paper_00032700_v82_n13_p5518_Gholipour |
| _version_ |
1768542913889828864 |