Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis

Several sugar phosphates have been converted to cyclic phosphates or methyl esters by treatment with dicyclohexylcarbodiimide in methanolic solution. The products obtained were then separated by paper chromatography with solvents containing cetyltrimethylammonium bromide or by paper electrophoresis...

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Autor principal: Cabib, Enrique
Publicado: 1963
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v35_n6_p755_Piras
http://hdl.handle.net/20.500.12110/paper_00032700_v35_n6_p755_Piras
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00032700_v35_n6_p755_Piras
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spelling paper:paper_00032700_v35_n6_p755_Piras2023-06-08T14:24:07Z Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis Cabib, Enrique Several sugar phosphates have been converted to cyclic phosphates or methyl esters by treatment with dicyclohexylcarbodiimide in methanolic solution. The products obtained were then separated by paper chromatography with solvents containing cetyltrimethylammonium bromide or by paper electrophoresis in potassium or cetyltrimethylammonium borate buffers. The separations obtained were better than those previously described for the untreated esters and the complete procedure could be carried out with less than 0.5 μmole of substance. The compounds obtained in the dicyclohexylcarbodiimide reaction were studied in detail and general rules have been formulated for the prediction of the type of product to be expected in each case. Some correlations between chemical structure and chromatographic or electrophoretic behavior have been observed. © 1963, American Chemical Society. All rights reserved. Fil:Cabib, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1963 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v35_n6_p755_Piras http://hdl.handle.net/20.500.12110/paper_00032700_v35_n6_p755_Piras
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Several sugar phosphates have been converted to cyclic phosphates or methyl esters by treatment with dicyclohexylcarbodiimide in methanolic solution. The products obtained were then separated by paper chromatography with solvents containing cetyltrimethylammonium bromide or by paper electrophoresis in potassium or cetyltrimethylammonium borate buffers. The separations obtained were better than those previously described for the untreated esters and the complete procedure could be carried out with less than 0.5 μmole of substance. The compounds obtained in the dicyclohexylcarbodiimide reaction were studied in detail and general rules have been formulated for the prediction of the type of product to be expected in each case. Some correlations between chemical structure and chromatographic or electrophoretic behavior have been observed. © 1963, American Chemical Society. All rights reserved.
author Cabib, Enrique
spellingShingle Cabib, Enrique
Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
author_facet Cabib, Enrique
author_sort Cabib, Enrique
title Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_short Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_full Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_fullStr Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_full_unstemmed Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_sort microscale identification of several sugar phosphates by paper chromatography and electrophoresis
publishDate 1963
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v35_n6_p755_Piras
http://hdl.handle.net/20.500.12110/paper_00032700_v35_n6_p755_Piras
work_keys_str_mv AT cabibenrique microscaleidentificationofseveralsugarphosphatesbypaperchromatographyandelectrophoresis
_version_ 1768541634780200960

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