A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis

A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity pattern...

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Autores principales:	Krisman de Fischman, Clara Rebeca, Blumenfeld, Marta Liliana
Publicado:	1986
Materias:	Glycogen synthase assay glycogen synthase gel electrophoresis glycogen synthase polyacrylamide gel detection iodine staining α-1,4 glucan synthase autoradiography α-1,4 glucan synthase iodine staining glycogen synthase enzyme assay methodology nonhuman polyacrylamide gel electrophoresis priority journal alpha-Amylase Animal Autoradiography Electrophoresis, Polyacrylamide Gel Glycogen Synthase Iodine Myocardium Rats Solutions Staining and Labeling Support, Non-U.S. Gov't Time Factors
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v154_n2_p409_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v154_n2_p409_Krisman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00032697_v154_n2_p409_Krisman
record_format	dspace
spelling	paper:paper_00032697_v154_n2_p409_Krisman2023-06-08T14:24:00Z A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis Krisman de Fischman, Clara Rebeca Blumenfeld, Marta Liliana Glycogen synthase assay glycogen synthase gel electrophoresis glycogen synthase polyacrylamide gel detection iodine staining α-1,4 glucan synthase autoradiography α-1,4 glucan synthase iodine staining glycogen synthase enzyme assay methodology nonhuman polyacrylamide gel electrophoresis priority journal alpha-Amylase Animal Autoradiography Electrophoresis, Polyacrylamide Gel Glycogen Synthase Iodine Myocardium Rats Solutions Staining and Labeling Support, Non-U.S. Gov't Time Factors A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity patterns were obtained by autoradiography or iodine staining of the gels. The method here described offers several advantages in terms of speed, sensitivity, and economy when compared with other procedures. © 1986. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Blumenfeld, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v154_n2_p409_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v154_n2_p409_Krisman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Glycogen synthase assay glycogen synthase gel electrophoresis glycogen synthase polyacrylamide gel detection iodine staining α-1,4 glucan synthase autoradiography α-1,4 glucan synthase iodine staining glycogen synthase enzyme assay methodology nonhuman polyacrylamide gel electrophoresis priority journal alpha-Amylase Animal Autoradiography Electrophoresis, Polyacrylamide Gel Glycogen Synthase Iodine Myocardium Rats Solutions Staining and Labeling Support, Non-U.S. Gov't Time Factors
spellingShingle	Glycogen synthase assay glycogen synthase gel electrophoresis glycogen synthase polyacrylamide gel detection iodine staining α-1,4 glucan synthase autoradiography α-1,4 glucan synthase iodine staining glycogen synthase enzyme assay methodology nonhuman polyacrylamide gel electrophoresis priority journal alpha-Amylase Animal Autoradiography Electrophoresis, Polyacrylamide Gel Glycogen Synthase Iodine Myocardium Rats Solutions Staining and Labeling Support, Non-U.S. Gov't Time Factors Krisman de Fischman, Clara Rebeca Blumenfeld, Marta Liliana A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
topic_facet	Glycogen synthase assay glycogen synthase gel electrophoresis glycogen synthase polyacrylamide gel detection iodine staining α-1,4 glucan synthase autoradiography α-1,4 glucan synthase iodine staining glycogen synthase enzyme assay methodology nonhuman polyacrylamide gel electrophoresis priority journal alpha-Amylase Animal Autoradiography Electrophoresis, Polyacrylamide Gel Glycogen Synthase Iodine Myocardium Rats Solutions Staining and Labeling Support, Non-U.S. Gov't Time Factors
description	A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity patterns were obtained by autoradiography or iodine staining of the gels. The method here described offers several advantages in terms of speed, sensitivity, and economy when compared with other procedures. © 1986.
author	Krisman de Fischman, Clara Rebeca Blumenfeld, Marta Liliana
author_facet	Krisman de Fischman, Clara Rebeca Blumenfeld, Marta Liliana
author_sort	Krisman de Fischman, Clara Rebeca
title	A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_short	A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_full	A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_fullStr	A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_full_unstemmed	A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
title_sort	method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis
publishDate	1986
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v154_n2_p409_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v154_n2_p409_Krisman
work_keys_str_mv	AT krismandefischmanclararebeca amethodforthedirectmeasurementofglycogensynthaseactivityongelsafterpolyacrylamidegelelectrophoresis AT blumenfeldmartaliliana amethodforthedirectmeasurementofglycogensynthaseactivityongelsafterpolyacrylamidegelelectrophoresis AT krismandefischmanclararebeca methodforthedirectmeasurementofglycogensynthaseactivityongelsafterpolyacrylamidegelelectrophoresis AT blumenfeldmartaliliana methodforthedirectmeasurementofglycogensynthaseactivityongelsafterpolyacrylamidegelelectrophoresis
_version_	1768545580788744192

A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis

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