Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points

Methods previously described for glycogen or amylopectin branching enzymatic activity are insufficiently sensitive and not quantitative. A new, more sensitive, specific, and quantitative one was developed. It is based upon the quantitation of the glucose residues joined by α1,6 bonds introduced by v...

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Autores principales:	Krisman de Fischman, Clara Rebeca, Tolmasky, Diana Silvia
Publicado:	1985
Materias:	amylo-α1,4-α1,6-transglucosylase amylopectin branching enzyme assay glycogen quantitation of branching points α1,6-glucosydic linkage 1,4 alpha glucan branching enzyme enzyme assay nonhuman priority journal 1,4-alpha-Glucan Branching Enzyme Animal Glucans Glucosyltransferases Polysaccharides Rabbits
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v147_n2_p491_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v147_n2_p491_Krisman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00032697_v147_n2_p491_Krisman
record_format	dspace
spelling	paper:paper_00032697_v147_n2_p491_Krisman2023-06-08T14:23:59Z Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points Krisman de Fischman, Clara Rebeca Tolmasky, Diana Silvia amylo-α1,4-α1,6-transglucosylase amylopectin branching enzyme assay glycogen quantitation of branching points α1,6-glucosydic linkage 1,4 alpha glucan branching enzyme enzyme assay nonhuman priority journal 1,4-alpha-Glucan Branching Enzyme Animal Glucans Glucosyltransferases Polysaccharides Rabbits Methods previously described for glycogen or amylopectin branching enzymatic activity are insufficiently sensitive and not quantitative. A new, more sensitive, specific, and quantitative one was developed. It is based upon the quantitation of the glucose residues joined by α1,6 bonds introduced by varying amounts of branching enzyme. The procedure involved the synthesis of a polysaccharide from Glc-1-P and phosphorylase in the presence of the sample to be tested. The branched polysaccharide was then purified and the glucoses involved in the branching points were quantitated after degradation with phosphorylase and debranching enzymes. This method appeared to be useful, not only in enzymatic activity determinations but also in the study of the structure of α-d-glucans when combined with those of total polysaccharide quantitation, such as iodine and phenol-sulfuric acid. © 1985. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tolmasky, D.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v147_n2_p491_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v147_n2_p491_Krisman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	amylo-α1,4-α1,6-transglucosylase amylopectin branching enzyme assay glycogen quantitation of branching points α1,6-glucosydic linkage 1,4 alpha glucan branching enzyme enzyme assay nonhuman priority journal 1,4-alpha-Glucan Branching Enzyme Animal Glucans Glucosyltransferases Polysaccharides Rabbits
spellingShingle	amylo-α1,4-α1,6-transglucosylase amylopectin branching enzyme assay glycogen quantitation of branching points α1,6-glucosydic linkage 1,4 alpha glucan branching enzyme enzyme assay nonhuman priority journal 1,4-alpha-Glucan Branching Enzyme Animal Glucans Glucosyltransferases Polysaccharides Rabbits Krisman de Fischman, Clara Rebeca Tolmasky, Diana Silvia Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
topic_facet	amylo-α1,4-α1,6-transglucosylase amylopectin branching enzyme assay glycogen quantitation of branching points α1,6-glucosydic linkage 1,4 alpha glucan branching enzyme enzyme assay nonhuman priority journal 1,4-alpha-Glucan Branching Enzyme Animal Glucans Glucosyltransferases Polysaccharides Rabbits
description	Methods previously described for glycogen or amylopectin branching enzymatic activity are insufficiently sensitive and not quantitative. A new, more sensitive, specific, and quantitative one was developed. It is based upon the quantitation of the glucose residues joined by α1,6 bonds introduced by varying amounts of branching enzyme. The procedure involved the synthesis of a polysaccharide from Glc-1-P and phosphorylase in the presence of the sample to be tested. The branched polysaccharide was then purified and the glucoses involved in the branching points were quantitated after degradation with phosphorylase and debranching enzymes. This method appeared to be useful, not only in enzymatic activity determinations but also in the study of the structure of α-d-glucans when combined with those of total polysaccharide quantitation, such as iodine and phenol-sulfuric acid. © 1985.
author	Krisman de Fischman, Clara Rebeca Tolmasky, Diana Silvia
author_facet	Krisman de Fischman, Clara Rebeca Tolmasky, Diana Silvia
author_sort	Krisman de Fischman, Clara Rebeca
title	Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_short	Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_full	Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_fullStr	Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_full_unstemmed	Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_sort	branching enzyme assay: selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
publishDate	1985
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v147_n2_p491_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v147_n2_p491_Krisman
work_keys_str_mv	AT krismandefischmanclararebeca branchingenzymeassayselectivequantitationofthea16linkedglucosylresiduesinvolvedinthebranchingpoints AT tolmaskydianasilvia branchingenzymeassayselectivequantitationofthea16linkedglucosylresiduesinvolvedinthebranchingpoints
_version_	1768541729405796352

Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points

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