Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease
It is still unclear to what extent myocarditis-associated, chronic Chagas' heart disease is due to persisting Trypanosoma cruzi. In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the pres...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v68_n2_p242_Elias http://hdl.handle.net/20.500.12110/paper_00029637_v68_n2_p242_Elias |
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paper:paper_00029637_v68_n2_p242_Elias2023-06-08T14:23:18Z Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease cell nucleus DNA kinetoplast DNA protozoal DNA primer DNA protozoal DNA adult animal experiment animal model animal tissue article case report Chagas disease controlled study female heart human human tissue in situ hybridization inflammation macrophage male mouse myocarditis nonhuman persistent infection tissue section Trypanosoma cruzi zoonosis animal Bagg albino mouse Chagas disease chronic disease genetics heart muscle isolation and purification middle aged molecular genetics myocarditis nucleotide sequence parasitology pathology polymerase chain reaction Animalia Trypanosoma Trypanosoma cruzi Animals Base Sequence Chagas Cardiomyopathy Chronic Disease DNA Primers DNA, Protozoan Female Humans Male Mice Mice, Inbred BALB C Middle Aged Molecular Sequence Data Myocarditis Myocardium Polymerase Chain Reaction Trypanosoma cruzi It is still unclear to what extent myocarditis-associated, chronic Chagas' heart disease is due to persisting Trypanosoma cruzi. In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the presence of intact T. cruzi, even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both T. cruzi kinetoplast DNA and nuclear DNA. Trypanosoma cruzi DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that T. cruzi kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. Since we rarely detected intact T. cruzi parasites during the chronic phase of Chagas' heart disease, we can exclude heart tissue as a major parasite reservoir. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v68_n2_p242_Elias http://hdl.handle.net/20.500.12110/paper_00029637_v68_n2_p242_Elias |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cell nucleus DNA kinetoplast DNA protozoal DNA primer DNA protozoal DNA adult animal experiment animal model animal tissue article case report Chagas disease controlled study female heart human human tissue in situ hybridization inflammation macrophage male mouse myocarditis nonhuman persistent infection tissue section Trypanosoma cruzi zoonosis animal Bagg albino mouse Chagas disease chronic disease genetics heart muscle isolation and purification middle aged molecular genetics myocarditis nucleotide sequence parasitology pathology polymerase chain reaction Animalia Trypanosoma Trypanosoma cruzi Animals Base Sequence Chagas Cardiomyopathy Chronic Disease DNA Primers DNA, Protozoan Female Humans Male Mice Mice, Inbred BALB C Middle Aged Molecular Sequence Data Myocarditis Myocardium Polymerase Chain Reaction Trypanosoma cruzi |
| spellingShingle |
cell nucleus DNA kinetoplast DNA protozoal DNA primer DNA protozoal DNA adult animal experiment animal model animal tissue article case report Chagas disease controlled study female heart human human tissue in situ hybridization inflammation macrophage male mouse myocarditis nonhuman persistent infection tissue section Trypanosoma cruzi zoonosis animal Bagg albino mouse Chagas disease chronic disease genetics heart muscle isolation and purification middle aged molecular genetics myocarditis nucleotide sequence parasitology pathology polymerase chain reaction Animalia Trypanosoma Trypanosoma cruzi Animals Base Sequence Chagas Cardiomyopathy Chronic Disease DNA Primers DNA, Protozoan Female Humans Male Mice Mice, Inbred BALB C Middle Aged Molecular Sequence Data Myocarditis Myocardium Polymerase Chain Reaction Trypanosoma cruzi Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease |
| topic_facet |
cell nucleus DNA kinetoplast DNA protozoal DNA primer DNA protozoal DNA adult animal experiment animal model animal tissue article case report Chagas disease controlled study female heart human human tissue in situ hybridization inflammation macrophage male mouse myocarditis nonhuman persistent infection tissue section Trypanosoma cruzi zoonosis animal Bagg albino mouse Chagas disease chronic disease genetics heart muscle isolation and purification middle aged molecular genetics myocarditis nucleotide sequence parasitology pathology polymerase chain reaction Animalia Trypanosoma Trypanosoma cruzi Animals Base Sequence Chagas Cardiomyopathy Chronic Disease DNA Primers DNA, Protozoan Female Humans Male Mice Mice, Inbred BALB C Middle Aged Molecular Sequence Data Myocarditis Myocardium Polymerase Chain Reaction Trypanosoma cruzi |
| description |
It is still unclear to what extent myocarditis-associated, chronic Chagas' heart disease is due to persisting Trypanosoma cruzi. In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the presence of intact T. cruzi, even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both T. cruzi kinetoplast DNA and nuclear DNA. Trypanosoma cruzi DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that T. cruzi kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. Since we rarely detected intact T. cruzi parasites during the chronic phase of Chagas' heart disease, we can exclude heart tissue as a major parasite reservoir. |
| title |
Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease |
| title_short |
Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease |
| title_full |
Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease |
| title_fullStr |
Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease |
| title_full_unstemmed |
Analysis of the presence of Trypanosoma cruzi in the heart tissue of three patients with chronic Chagas' heart disease |
| title_sort |
analysis of the presence of trypanosoma cruzi in the heart tissue of three patients with chronic chagas' heart disease |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00029637_v68_n2_p242_Elias http://hdl.handle.net/20.500.12110/paper_00029637_v68_n2_p242_Elias |
| _version_ |
1768545533116284928 |