Wired-enzyme core-shell Au nanoparticle biosensor
We report a fully integrated core-shell nanoparticle system responsive to glucose. The system is comprised of self-assembled glucose oxidase and an osmium molecular wire on core-shell Au nanoparticles. Characterization of the functional nanoparticles by spectroscopy, quartz crystal microbalance and...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v130_n38_p12690_Scodeller http://hdl.handle.net/20.500.12110/paper_00027863_v130_n38_p12690_Scodeller |
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paper:paper_00027863_v130_n38_p12690_Scodeller2023-06-08T14:22:44Z Wired-enzyme core-shell Au nanoparticle biosensor Scodeller, Pablo David Flexer, Victoria Calvo, Ernesto Julio Biosensors Computer operating systems Crystal structure Food additives Glucose Glucose oxidase Glucose sensors Nanoparticles Nanostructured materials Nanostructures Osmium Oxide minerals Quartz Quartz crystal microbalances Reduction Shells (structures) Amperometric Au nano-particles Bio sensor Bio-sensing Contactless Core shells Electrochemical techniques Enzymatic reduction Fully integrated Functional nanoparticles Glucose oxidases Molecular wires Photonic Quartz crystal micro-balance Reagent-less Resonant Raman Self-assembled Self-assembled multilayer Gold flavine adenine nucleotide flavine mononucleotide reductase glucose glucose oxidase gold nanoparticle nanowire osmium silicon dioxide amperometry article biosensor calibration catalysis electrochemical analysis enzyme substrate complex oxidation Raman spectrometry reduction signal transduction spectroscopy surface property synthesis Biosensing Techniques Calibration Electrochemical Techniques Flavin-Adenine Dinucleotide Glucose Glucose Oxidase Gold Metal Nanoparticles Microscopy, Atomic Force Microscopy, Electron, Transmission Nanowires Osmium Spectrophotometry, Ultraviolet Spectrum Analysis, Raman We report a fully integrated core-shell nanoparticle system responsive to glucose. The system is comprised of self-assembled glucose oxidase and an osmium molecular wire on core-shell Au nanoparticles. Characterization of the functional nanoparticles by spectroscopy, quartz crystal microbalance and electrochemical techniques has shown that the catalytically active shell has a structure as designed and all components are active in the self-assembled multilayer shell. Furthermore, amperometric reagentless detection of glucose and contactless photonic biosensing by the Os(II) resonant Raman signal have been demonstrated. The enzymatic reduction of FAD by glucose and further reduction of the Raman silent Os(III) by FADH2 yields a characteristic enzyme-substrate calibration curve in the millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra enhancement of the biosensor signal. We present a proof of concept extending the work done with planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected by SERS-like methods. © 2008 American Chemical Society. Fil:Scodeller, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flexer, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v130_n38_p12690_Scodeller http://hdl.handle.net/20.500.12110/paper_00027863_v130_n38_p12690_Scodeller |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biosensors Computer operating systems Crystal structure Food additives Glucose Glucose oxidase Glucose sensors Nanoparticles Nanostructured materials Nanostructures Osmium Oxide minerals Quartz Quartz crystal microbalances Reduction Shells (structures) Amperometric Au nano-particles Bio sensor Bio-sensing Contactless Core shells Electrochemical techniques Enzymatic reduction Fully integrated Functional nanoparticles Glucose oxidases Molecular wires Photonic Quartz crystal micro-balance Reagent-less Resonant Raman Self-assembled Self-assembled multilayer Gold flavine adenine nucleotide flavine mononucleotide reductase glucose glucose oxidase gold nanoparticle nanowire osmium silicon dioxide amperometry article biosensor calibration catalysis electrochemical analysis enzyme substrate complex oxidation Raman spectrometry reduction signal transduction spectroscopy surface property synthesis Biosensing Techniques Calibration Electrochemical Techniques Flavin-Adenine Dinucleotide Glucose Glucose Oxidase Gold Metal Nanoparticles Microscopy, Atomic Force Microscopy, Electron, Transmission Nanowires Osmium Spectrophotometry, Ultraviolet Spectrum Analysis, Raman |
| spellingShingle |
Biosensors Computer operating systems Crystal structure Food additives Glucose Glucose oxidase Glucose sensors Nanoparticles Nanostructured materials Nanostructures Osmium Oxide minerals Quartz Quartz crystal microbalances Reduction Shells (structures) Amperometric Au nano-particles Bio sensor Bio-sensing Contactless Core shells Electrochemical techniques Enzymatic reduction Fully integrated Functional nanoparticles Glucose oxidases Molecular wires Photonic Quartz crystal micro-balance Reagent-less Resonant Raman Self-assembled Self-assembled multilayer Gold flavine adenine nucleotide flavine mononucleotide reductase glucose glucose oxidase gold nanoparticle nanowire osmium silicon dioxide amperometry article biosensor calibration catalysis electrochemical analysis enzyme substrate complex oxidation Raman spectrometry reduction signal transduction spectroscopy surface property synthesis Biosensing Techniques Calibration Electrochemical Techniques Flavin-Adenine Dinucleotide Glucose Glucose Oxidase Gold Metal Nanoparticles Microscopy, Atomic Force Microscopy, Electron, Transmission Nanowires Osmium Spectrophotometry, Ultraviolet Spectrum Analysis, Raman Scodeller, Pablo David Flexer, Victoria Calvo, Ernesto Julio Wired-enzyme core-shell Au nanoparticle biosensor |
| topic_facet |
Biosensors Computer operating systems Crystal structure Food additives Glucose Glucose oxidase Glucose sensors Nanoparticles Nanostructured materials Nanostructures Osmium Oxide minerals Quartz Quartz crystal microbalances Reduction Shells (structures) Amperometric Au nano-particles Bio sensor Bio-sensing Contactless Core shells Electrochemical techniques Enzymatic reduction Fully integrated Functional nanoparticles Glucose oxidases Molecular wires Photonic Quartz crystal micro-balance Reagent-less Resonant Raman Self-assembled Self-assembled multilayer Gold flavine adenine nucleotide flavine mononucleotide reductase glucose glucose oxidase gold nanoparticle nanowire osmium silicon dioxide amperometry article biosensor calibration catalysis electrochemical analysis enzyme substrate complex oxidation Raman spectrometry reduction signal transduction spectroscopy surface property synthesis Biosensing Techniques Calibration Electrochemical Techniques Flavin-Adenine Dinucleotide Glucose Glucose Oxidase Gold Metal Nanoparticles Microscopy, Atomic Force Microscopy, Electron, Transmission Nanowires Osmium Spectrophotometry, Ultraviolet Spectrum Analysis, Raman |
| description |
We report a fully integrated core-shell nanoparticle system responsive to glucose. The system is comprised of self-assembled glucose oxidase and an osmium molecular wire on core-shell Au nanoparticles. Characterization of the functional nanoparticles by spectroscopy, quartz crystal microbalance and electrochemical techniques has shown that the catalytically active shell has a structure as designed and all components are active in the self-assembled multilayer shell. Furthermore, amperometric reagentless detection of glucose and contactless photonic biosensing by the Os(II) resonant Raman signal have been demonstrated. The enzymatic reduction of FAD by glucose and further reduction of the Raman silent Os(III) by FADH2 yields a characteristic enzyme-substrate calibration curve in the millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra enhancement of the biosensor signal. We present a proof of concept extending the work done with planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected by SERS-like methods. © 2008 American Chemical Society. |
| author |
Scodeller, Pablo David Flexer, Victoria Calvo, Ernesto Julio |
| author_facet |
Scodeller, Pablo David Flexer, Victoria Calvo, Ernesto Julio |
| author_sort |
Scodeller, Pablo David |
| title |
Wired-enzyme core-shell Au nanoparticle biosensor |
| title_short |
Wired-enzyme core-shell Au nanoparticle biosensor |
| title_full |
Wired-enzyme core-shell Au nanoparticle biosensor |
| title_fullStr |
Wired-enzyme core-shell Au nanoparticle biosensor |
| title_full_unstemmed |
Wired-enzyme core-shell Au nanoparticle biosensor |
| title_sort |
wired-enzyme core-shell au nanoparticle biosensor |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v130_n38_p12690_Scodeller http://hdl.handle.net/20.500.12110/paper_00027863_v130_n38_p12690_Scodeller |
| work_keys_str_mv |
AT scodellerpablodavid wiredenzymecoreshellaunanoparticlebiosensor AT flexervictoria wiredenzymecoreshellaunanoparticlebiosensor AT calvoernestojulio wiredenzymecoreshellaunanoparticlebiosensor |
| _version_ |
1768542670135754752 |