Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco
Triatoma sordida is a secondary vector of Trypanosoma cruzi in the Gran Chaco and Cerrado eco-regions where it frequently infests peridomestic and domestic habitats. In a well-defined area of the humid Argentine Chaco, very few T. sordida were found infected when examined by optical microscopic exam...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v143_n_p97_Macchiaverna http://hdl.handle.net/20.500.12110/paper_0001706X_v143_n_p97_Macchiaverna |
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paper:paper_0001706X_v143_n_p97_Macchiaverna2023-06-08T14:21:15Z Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco Bloodmeal identification DTU Molecular diagnosis Triatoma sordida Trypanosoma cruzi Vector control insecticide antitrypanosomal agent insecticide kinetoplast DNA protozoal DNA disease control disease incidence disease prevalence disease transmission disease vector humid tropics infectious disease insecticide parasitic disease Argentina Article Chagas disease chicken controlled study disease transmission habitat infestation microscopy nonhuman opossum polymerase chain reaction prevalence rodent Triatoma infestans Triatoma sordida Trypanosoma cruzi turkey (bird) animal Chagas disease disease carrier domestic animal genetics parasitology physiology transmission Triatoma Trypanosoma cruzi Argentina Chaco [Argentina] Aves Didelphidae Gallus gallus Hexapoda Rodentia Triatoma sordida Trypanosoma cruzi Animals Animals, Domestic Argentina Chagas Disease Chickens DNA, Kinetoplast DNA, Protozoan Insect Vectors Insecticides Polymerase Chain Reaction Prevalence Triatoma Trypanocidal Agents Trypanosoma cruzi Turkeys Triatoma sordida is a secondary vector of Trypanosoma cruzi in the Gran Chaco and Cerrado eco-regions where it frequently infests peridomestic and domestic habitats. In a well-defined area of the humid Argentine Chaco, very few T. sordida were found infected when examined by optical microscopic examination (OM). In order to further assess the role of T. sordida and the relative magnitude of subpatent bug infections, we examined the insects for T. cruzi infection, parasite Discrete Typing Units (DTUs) and bloodmeal sources using various molecular techniques. Among 205 bugs with a negative or no OM-based diagnosis, the prevalence of infection determined by kDNA-PCR was nearly the same in bugs captured before (6.3%) and 4 months after insecticide spraying (6.4%). On average, these estimates were sixfold higher than the prevalence of infection based on OM (1.1%). Only TcI was identified, a DTU typically associated with opossums and rodents. Chickens and turkeys were the only bloodmeal sources identified in the infected specimens and the main local hosts at the bugs' capture sites. As birds are refractory to T. cruzi infection, further studies are needed to identify the infectious bloodmeal hosts. The persistent finding of infected T. sordida after community-wide insecticide spraying highlights the need of sustained vector surveillance to effectively prevent T. cruzi transmission in the domestic and peridomestic habitats. © 2014 Elsevier B.V.. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v143_n_p97_Macchiaverna http://hdl.handle.net/20.500.12110/paper_0001706X_v143_n_p97_Macchiaverna |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bloodmeal identification DTU Molecular diagnosis Triatoma sordida Trypanosoma cruzi Vector control insecticide antitrypanosomal agent insecticide kinetoplast DNA protozoal DNA disease control disease incidence disease prevalence disease transmission disease vector humid tropics infectious disease insecticide parasitic disease Argentina Article Chagas disease chicken controlled study disease transmission habitat infestation microscopy nonhuman opossum polymerase chain reaction prevalence rodent Triatoma infestans Triatoma sordida Trypanosoma cruzi turkey (bird) animal Chagas disease disease carrier domestic animal genetics parasitology physiology transmission Triatoma Trypanosoma cruzi Argentina Chaco [Argentina] Aves Didelphidae Gallus gallus Hexapoda Rodentia Triatoma sordida Trypanosoma cruzi Animals Animals, Domestic Argentina Chagas Disease Chickens DNA, Kinetoplast DNA, Protozoan Insect Vectors Insecticides Polymerase Chain Reaction Prevalence Triatoma Trypanocidal Agents Trypanosoma cruzi Turkeys |
| spellingShingle |
Bloodmeal identification DTU Molecular diagnosis Triatoma sordida Trypanosoma cruzi Vector control insecticide antitrypanosomal agent insecticide kinetoplast DNA protozoal DNA disease control disease incidence disease prevalence disease transmission disease vector humid tropics infectious disease insecticide parasitic disease Argentina Article Chagas disease chicken controlled study disease transmission habitat infestation microscopy nonhuman opossum polymerase chain reaction prevalence rodent Triatoma infestans Triatoma sordida Trypanosoma cruzi turkey (bird) animal Chagas disease disease carrier domestic animal genetics parasitology physiology transmission Triatoma Trypanosoma cruzi Argentina Chaco [Argentina] Aves Didelphidae Gallus gallus Hexapoda Rodentia Triatoma sordida Trypanosoma cruzi Animals Animals, Domestic Argentina Chagas Disease Chickens DNA, Kinetoplast DNA, Protozoan Insect Vectors Insecticides Polymerase Chain Reaction Prevalence Triatoma Trypanocidal Agents Trypanosoma cruzi Turkeys Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco |
| topic_facet |
Bloodmeal identification DTU Molecular diagnosis Triatoma sordida Trypanosoma cruzi Vector control insecticide antitrypanosomal agent insecticide kinetoplast DNA protozoal DNA disease control disease incidence disease prevalence disease transmission disease vector humid tropics infectious disease insecticide parasitic disease Argentina Article Chagas disease chicken controlled study disease transmission habitat infestation microscopy nonhuman opossum polymerase chain reaction prevalence rodent Triatoma infestans Triatoma sordida Trypanosoma cruzi turkey (bird) animal Chagas disease disease carrier domestic animal genetics parasitology physiology transmission Triatoma Trypanosoma cruzi Argentina Chaco [Argentina] Aves Didelphidae Gallus gallus Hexapoda Rodentia Triatoma sordida Trypanosoma cruzi Animals Animals, Domestic Argentina Chagas Disease Chickens DNA, Kinetoplast DNA, Protozoan Insect Vectors Insecticides Polymerase Chain Reaction Prevalence Triatoma Trypanocidal Agents Trypanosoma cruzi Turkeys |
| description |
Triatoma sordida is a secondary vector of Trypanosoma cruzi in the Gran Chaco and Cerrado eco-regions where it frequently infests peridomestic and domestic habitats. In a well-defined area of the humid Argentine Chaco, very few T. sordida were found infected when examined by optical microscopic examination (OM). In order to further assess the role of T. sordida and the relative magnitude of subpatent bug infections, we examined the insects for T. cruzi infection, parasite Discrete Typing Units (DTUs) and bloodmeal sources using various molecular techniques. Among 205 bugs with a negative or no OM-based diagnosis, the prevalence of infection determined by kDNA-PCR was nearly the same in bugs captured before (6.3%) and 4 months after insecticide spraying (6.4%). On average, these estimates were sixfold higher than the prevalence of infection based on OM (1.1%). Only TcI was identified, a DTU typically associated with opossums and rodents. Chickens and turkeys were the only bloodmeal sources identified in the infected specimens and the main local hosts at the bugs' capture sites. As birds are refractory to T. cruzi infection, further studies are needed to identify the infectious bloodmeal hosts. The persistent finding of infected T. sordida after community-wide insecticide spraying highlights the need of sustained vector surveillance to effectively prevent T. cruzi transmission in the domestic and peridomestic habitats. © 2014 Elsevier B.V.. |
| title |
Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco |
| title_short |
Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco |
| title_full |
Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco |
| title_fullStr |
Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco |
| title_full_unstemmed |
Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco |
| title_sort |
trypanosoma cruzi infection in triatoma sordida before and after community-wide residual insecticide spraying in the argentinean chaco |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v143_n_p97_Macchiaverna http://hdl.handle.net/20.500.12110/paper_0001706X_v143_n_p97_Macchiaverna |
| _version_ |
1768543060780646400 |