Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina

Introduction. Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological...

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Autores principales: Gertiser, María Laura, Giagante, E., Sgattoni, E., Basabe, N., Rivero, Fernando David, Luján, Hugo Daniel, Occhionero, M., Pannicia, L., Visciarelli, E., Costamagna, Sixto
Formato: Artículo acceptedVersion
Lenguaje:Español
Publicado: Asociación Argentina de Microbiología 2010
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QD Química
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3442/1/A_Gertiser_Giagante_Sgattoni_Basabe_Rivero_Luj%C3%A1n_Occhionero_Paniccia_Visciarelli_Costamagna.pdf
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Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3442
record_format dspace
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QD Química
spellingShingle QD Química
Gertiser, María Laura
Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Pannicia, L.
Visciarelli, E.
Costamagna, Sixto
Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
topic_facet QD Química
description Introduction. Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
format Artículo
Artículo
acceptedVersion
author Gertiser, María Laura
Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Pannicia, L.
Visciarelli, E.
Costamagna, Sixto
author_facet Gertiser, María Laura
Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Pannicia, L.
Visciarelli, E.
Costamagna, Sixto
author_sort Gertiser, María Laura
title Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_short Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_full Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_fullStr Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_full_unstemmed Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_sort queratitis por acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en bahía blanca, provincia de buenos aires, argentina
publisher Asociación Argentina de Microbiología
publishDate 2010
url http://pa.bibdigital.ucc.edu.ar/3442/1/A_Gertiser_Giagante_Sgattoni_Basabe_Rivero_Luj%C3%A1n_Occhionero_Paniccia_Visciarelli_Costamagna.pdf
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