Identificación de sustratos fosforilados por la PKA de Yarrowia lipolytica mediante técnicas de proteómica comparativa

The cAMP-dependent protein kinase (PKA) is an enzyme that engages the transference of a phosphate group of ATP to its target protein; its activity is regulated by intracellular cAMP concentration. In Yarrowia lipolytica, a dimorphic fungus of biotechnological interest, we found that PKA is inv...

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Detalles Bibliográficos
Autor principal: Díaz Ludovico, Ivo
Otros Autores: María Florencia Kronberg
Formato: Tesis
Lenguaje:Español
Publicado: Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Carrera Licenciatura de Ciencias Bológicas 2014
Materias:
transición levadura-micelio
yeast-mycelium transition
dimorfismo fúngico
fungal dimorphism
fosfoproteoma
phosphoproteome
Acceso en línea:http://repositorio.ub.edu.ar/handle/123456789/2444
Aporte de:
Repositorio Institucional - Universidad de Belgrano (UB) de Universidad de Belgrano
Descripción
Sumario:The cAMP-dependent protein kinase (PKA) is an enzyme that engages the transference of a phosphate group of ATP to its target protein; its activity is regulated by intracellular cAMP concentration. In Yarrowia lipolytica, a dimorphic fungus of biotechnological interest, we found that PKA is involved in morphogenesis, cell metabolism and adaptation to stress conditions. The regulatory and catalytic subunits are encoded by sole genes, RKA1 and TPK1, respectively. It was possible to identify three proteins: the self regulatory subunit of PKA, Rka1, a protein with homology to Saccharomyces cerevisiae ubiquinone biosynthesis monooxygenase CoQ6 and the translational elongation factor EF1-α.

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