Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting

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Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on trache...

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Autores principales:	Centanin, L., Dekanty, A., Romero, N., Irisarri, M., Gorr, T.A., Wappner, P.
Formato:	Artículo publishedVersion
Publicado:	2008
Materias:	DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_Centanin https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_15345807_v14_n4_p547_Centanin_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

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spelling	I28-R145-paper_15345807_v14_n4_p547_Centanin_oai2024-08-16 Centanin, L. Dekanty, A. Romero, N. Irisarri, M. Gorr, T.A. Wappner, P. 2008 Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-α homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting. © 2008 Elsevier Inc. All rights reserved. Fil:Centanin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dekanty, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romero, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Irisarri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wappner, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_Centanin info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Dev. Cell 2008;14(4):547-558 DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_15345807_v14_n4_p547_Centanin_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia
spellingShingle	DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia Centanin, L. Dekanty, A. Romero, N. Irisarri, M. Gorr, T.A. Wappner, P. Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
topic_facet	DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia
description	Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-α homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting. © 2008 Elsevier Inc. All rights reserved.
format	Artículo Artículo publishedVersion
author	Centanin, L. Dekanty, A. Romero, N. Irisarri, M. Gorr, T.A. Wappner, P.
author_facet	Centanin, L. Dekanty, A. Romero, N. Irisarri, M. Gorr, T.A. Wappner, P.
author_sort	Centanin, L.
title	Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_short	Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_full	Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_fullStr	Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_full_unstemmed	Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_sort	cell autonomy of hif effects in drosophila: tracheal cells sense hypoxia and induce terminal branch sprouting
publishDate	2008
url	http://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_Centanin https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_15345807_v14_n4_p547_Centanin_oai
work_keys_str_mv	AT centaninl cellautonomyofhifeffectsindrosophilatrachealcellssensehypoxiaandinduceterminalbranchsprouting AT dekantya cellautonomyofhifeffectsindrosophilatrachealcellssensehypoxiaandinduceterminalbranchsprouting AT romeron cellautonomyofhifeffectsindrosophilatrachealcellssensehypoxiaandinduceterminalbranchsprouting AT irisarrim cellautonomyofhifeffectsindrosophilatrachealcellssensehypoxiaandinduceterminalbranchsprouting AT gorrta cellautonomyofhifeffectsindrosophilatrachealcellssensehypoxiaandinduceterminalbranchsprouting AT wappnerp cellautonomyofhifeffectsindrosophilatrachealcellssensehypoxiaandinduceterminalbranchsprouting
_version_	1809356929488650240

Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting

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