RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia

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SUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO conjugase Ubc9. RSUME increases noncovalent binding o...

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Autores principales:	Carbia-Nagashima, A., Gerez, J., Perez-Castro, C., Paez-Pereda, M., Silberstein, S., Stalla, G.K., Holsboer, F., Arzt, E.
Formato:	Artículo publishedVersion
Publicado:	2007
Materias:	CELLBIO PROTEINS cell protein hypoxia inducible factor 1alpha I kappa B immunoglobulin enhancer binding protein protein RSUME protein Ubc9 SUMO 1 protein sumo 2 protein sumo 3 protein SUMO protein unclassified drug animal cell article cell function conjugation controlled study genetic transcription human human cell hypoxia mammal cell mouse nonhuman nucleotide sequence polymerization priority journal protein binding protein domain protein expression protein protein interaction protein stability protein structure rat regulatory mechanism sumoylation Amino Acid Sequence Animals Base Sequence Cell Hypoxia Cell Line, Tumor Cercopithecus aethiops Humans Hypoxia-Inducible Factor 1, alpha Subunit I-kappa B Kinase Molecular Sequence Data NF-kappa B Organ Specificity Protein Binding Small Ubiquitin-Related Modifier Proteins SUMO-1 Protein Transcription Factors Ubiquitin-Conjugating Enzymes Ubiquitins Mammalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00928674_v131_n2_p309_CarbiaNagashima http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00928674_v131_n2_p309_CarbiaNagashima_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

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spelling	I28-R145-paper_00928674_v131_n2_p309_CarbiaNagashima_oai2020-10-19 Carbia-Nagashima, A. Gerez, J. Perez-Castro, C. Paez-Pereda, M. Silberstein, S. Stalla, G.K. Holsboer, F. Arzt, E. 2007 SUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO conjugase Ubc9. RSUME increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation and SUMO polymerization. RSUME enhances the sumoylation of IkB in vitro and in cultured cells, leading to an inhibition of NF-kB transcriptional activity. RSUME is induced by hypoxia and enhances the sumoylation of HIF-1α, promoting its stabilization and transcriptional activity during hypoxia. Disruption of the RWD domain structure of RSUME demonstrates that this domain is critical for RSUME action. Together, these findings point to a central role of RSUME in the regulation of sumoylation and, hence, several critical regulatory pathways in mammalian cells. © 2007 Elsevier Inc. All rights reserved. Fil:Carbia-Nagashima, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gerez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perez-Castro, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paez-Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silberstein, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00928674_v131_n2_p309_CarbiaNagashima info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Cell 2007;131(2):309-323 CELLBIO PROTEINS cell protein hypoxia inducible factor 1alpha I kappa B immunoglobulin enhancer binding protein protein RSUME protein Ubc9 SUMO 1 protein sumo 2 protein sumo 3 protein SUMO protein unclassified drug animal cell article cell function conjugation controlled study genetic transcription human human cell hypoxia mammal cell mouse nonhuman nucleotide sequence polymerization priority journal protein binding protein domain protein expression protein protein interaction protein stability protein structure rat regulatory mechanism sumoylation Amino Acid Sequence Animals Base Sequence Cell Hypoxia Cell Line, Tumor Cercopithecus aethiops Humans Hypoxia-Inducible Factor 1, alpha Subunit I-kappa B Kinase Molecular Sequence Data NF-kappa B Organ Specificity Protein Binding Small Ubiquitin-Related Modifier Proteins SUMO-1 Protein Transcription Factors Ubiquitin-Conjugating Enzymes Ubiquitins Mammalia RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00928674_v131_n2_p309_CarbiaNagashima_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	CELLBIO PROTEINS cell protein hypoxia inducible factor 1alpha I kappa B immunoglobulin enhancer binding protein protein RSUME protein Ubc9 SUMO 1 protein sumo 2 protein sumo 3 protein SUMO protein unclassified drug animal cell article cell function conjugation controlled study genetic transcription human human cell hypoxia mammal cell mouse nonhuman nucleotide sequence polymerization priority journal protein binding protein domain protein expression protein protein interaction protein stability protein structure rat regulatory mechanism sumoylation Amino Acid Sequence Animals Base Sequence Cell Hypoxia Cell Line, Tumor Cercopithecus aethiops Humans Hypoxia-Inducible Factor 1, alpha Subunit I-kappa B Kinase Molecular Sequence Data NF-kappa B Organ Specificity Protein Binding Small Ubiquitin-Related Modifier Proteins SUMO-1 Protein Transcription Factors Ubiquitin-Conjugating Enzymes Ubiquitins Mammalia
spellingShingle	CELLBIO PROTEINS cell protein hypoxia inducible factor 1alpha I kappa B immunoglobulin enhancer binding protein protein RSUME protein Ubc9 SUMO 1 protein sumo 2 protein sumo 3 protein SUMO protein unclassified drug animal cell article cell function conjugation controlled study genetic transcription human human cell hypoxia mammal cell mouse nonhuman nucleotide sequence polymerization priority journal protein binding protein domain protein expression protein protein interaction protein stability protein structure rat regulatory mechanism sumoylation Amino Acid Sequence Animals Base Sequence Cell Hypoxia Cell Line, Tumor Cercopithecus aethiops Humans Hypoxia-Inducible Factor 1, alpha Subunit I-kappa B Kinase Molecular Sequence Data NF-kappa B Organ Specificity Protein Binding Small Ubiquitin-Related Modifier Proteins SUMO-1 Protein Transcription Factors Ubiquitin-Conjugating Enzymes Ubiquitins Mammalia Carbia-Nagashima, A. Gerez, J. Perez-Castro, C. Paez-Pereda, M. Silberstein, S. Stalla, G.K. Holsboer, F. Arzt, E. RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
topic_facet	CELLBIO PROTEINS cell protein hypoxia inducible factor 1alpha I kappa B immunoglobulin enhancer binding protein protein RSUME protein Ubc9 SUMO 1 protein sumo 2 protein sumo 3 protein SUMO protein unclassified drug animal cell article cell function conjugation controlled study genetic transcription human human cell hypoxia mammal cell mouse nonhuman nucleotide sequence polymerization priority journal protein binding protein domain protein expression protein protein interaction protein stability protein structure rat regulatory mechanism sumoylation Amino Acid Sequence Animals Base Sequence Cell Hypoxia Cell Line, Tumor Cercopithecus aethiops Humans Hypoxia-Inducible Factor 1, alpha Subunit I-kappa B Kinase Molecular Sequence Data NF-kappa B Organ Specificity Protein Binding Small Ubiquitin-Related Modifier Proteins SUMO-1 Protein Transcription Factors Ubiquitin-Conjugating Enzymes Ubiquitins Mammalia
description	SUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO conjugase Ubc9. RSUME increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation and SUMO polymerization. RSUME enhances the sumoylation of IkB in vitro and in cultured cells, leading to an inhibition of NF-kB transcriptional activity. RSUME is induced by hypoxia and enhances the sumoylation of HIF-1α, promoting its stabilization and transcriptional activity during hypoxia. Disruption of the RWD domain structure of RSUME demonstrates that this domain is critical for RSUME action. Together, these findings point to a central role of RSUME in the regulation of sumoylation and, hence, several critical regulatory pathways in mammalian cells. © 2007 Elsevier Inc. All rights reserved.
format	Artículo Artículo publishedVersion
author	Carbia-Nagashima, A. Gerez, J. Perez-Castro, C. Paez-Pereda, M. Silberstein, S. Stalla, G.K. Holsboer, F. Arzt, E.
author_facet	Carbia-Nagashima, A. Gerez, J. Perez-Castro, C. Paez-Pereda, M. Silberstein, S. Stalla, G.K. Holsboer, F. Arzt, E.
author_sort	Carbia-Nagashima, A.
title	RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_short	RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_full	RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_fullStr	RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_full_unstemmed	RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_sort	rsume, a small rwd-containing protein, enhances sumo conjugation and stabilizes hif-1α during hypoxia
publishDate	2007
url	http://hdl.handle.net/20.500.12110/paper_00928674_v131_n2_p309_CarbiaNagashima http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00928674_v131_n2_p309_CarbiaNagashima_oai
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RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia

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