Folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on Zebrafish embryo and larvae

Doxorubicin (DOXO) is a chemotherapeutic agent widely used for the treatment of solid tumors and hematologic malignancies in both adults and children. However, DOXO causes short- and long-term cardiotoxicity and others undesirable side effects, such as nephrotoxicity and neurotoxicity. Magnetic nano...

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Autores principales: Igartúa, Daniela Edith, Azcona, Pamela Liliana, Martínez, Carolina Soledad, Alonso, Silvia del Valle, Lassalle, Verónica Leticia, Prieto, María Jimena
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Química
Biocompatibility
Developmental-toxicity
Doxorubicin
Magnetic nanoparticles
Zebrafish
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97506
https://ri.conicet.gov.ar/11336/94258
https://linkinghub.elsevier.com/retrieve/pii/S0041008X18304095
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97506
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biocompatibility
Developmental-toxicity
Doxorubicin
Magnetic nanoparticles
Zebrafish
spellingShingle Química
Biocompatibility
Developmental-toxicity
Doxorubicin
Magnetic nanoparticles
Zebrafish
Igartúa, Daniela Edith
Azcona, Pamela Liliana
Martínez, Carolina Soledad
Alonso, Silvia del Valle
Lassalle, Verónica Leticia
Prieto, María Jimena
Folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on Zebrafish embryo and larvae
topic_facet Química
Biocompatibility
Developmental-toxicity
Doxorubicin
Magnetic nanoparticles
Zebrafish
description Doxorubicin (DOXO) is a chemotherapeutic agent widely used for the treatment of solid tumors and hematologic malignancies in both adults and children. However, DOXO causes short- and long-term cardiotoxicity and others undesirable side effects, such as nephrotoxicity and neurotoxicity. Magnetic nanoparticles (MNPs) allow the delivery of drugs specifically to target place, employing an external magnet. Moreover, they may act as contrast agents in MRI providing information on the diagnostic of diverse pathologies. In this way, two functions may be combined in a unique nanosystem known as theranostic. Also, the MNPs can be modified with folic acid (MNPs@FA) to increase the uptake by cancer cells that overexpress the FA receptors. In previous works, our collaborators obtained and characterized MNPs, MNPs@FA, and MNPs@FA@DOXO. It is essential to study the biosafety of nanotheranostic, and there is no published study of Fe 3 O 4 nanoparticles developmental toxicity. Because of that, this work aimed to study the in vivo toxicity and biocompatibility of DOXO, MNPs@FA, and MNPs@FA@DOXO using zebrafish embryo and larvae as an animal model. Viability, developmental toxicity, changes in spontaneous movement (neurotoxicity), changes in cardiac rhythm (cardiotoxicity), and efficiency of DOXO-uptake were studied. While the 48-h treatment with 50 μg/mL of DOXO resulted in a 30% larvae death and the development of significant morphological abnormalities, the treatment with MNPs@FA@DOXO and MNPs@FA did not reduce the viability and did not cause developmental abnormalities. Besides, the MNPs@FA@DOXO reduced the cardiotoxicity and promoted a more rapid and significant uptake of DOXO by zebrafish larvae.
format Articulo
Preprint
author Igartúa, Daniela Edith
Azcona, Pamela Liliana
Martínez, Carolina Soledad
Alonso, Silvia del Valle
Lassalle, Verónica Leticia
Prieto, María Jimena
author_facet Igartúa, Daniela Edith
Azcona, Pamela Liliana
Martínez, Carolina Soledad
Alonso, Silvia del Valle
Lassalle, Verónica Leticia
Prieto, María Jimena
author_sort Igartúa, Daniela Edith
title Folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on Zebrafish embryo and larvae
title_short Folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on Zebrafish embryo and larvae
title_full Folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on Zebrafish embryo and larvae
title_fullStr Folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on Zebrafish embryo and larvae
title_full_unstemmed Folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on Zebrafish embryo and larvae
title_sort folic acid magnetic nanotheranostics for delivering doxorubicin: toxicological and biocompatibility studies on zebrafish embryo and larvae
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/97506
https://ri.conicet.gov.ar/11336/94258
https://linkinghub.elsevier.com/retrieve/pii/S0041008X18304095
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