Reversible nuclear-lipid-droplet morphology induced by oleic acid: A link to cellular-lipid metabolism

Neutral lipids - involved in many cellular processes - are stored as lipid droplets (LD), those mainly cytosolic (cLD) along with a small nuclear population (nLD). nLD could be involved in nuclear-lipid homeostasis serving as an endonuclear buffering system that would provide or incorporate lipids a...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lagrutta, Lucía Carolina, Montero Villegas, Sandra, Layerenza, Juan Pablo, Sisti, Martín Sebastián, García de Bravo, Margarita María, Ves Losada, Ana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Ciencias Médicas
lipids
metabolism
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87571
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87571
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Ciencias Médicas
lipids
metabolism
spellingShingle Ciencias Exactas
Ciencias Médicas
lipids
metabolism
Lagrutta, Lucía Carolina
Montero Villegas, Sandra
Layerenza, Juan Pablo
Sisti, Martín Sebastián
García de Bravo, Margarita María
Ves Losada, Ana
Reversible nuclear-lipid-droplet morphology induced by oleic acid: A link to cellular-lipid metabolism
topic_facet Ciencias Exactas
Ciencias Médicas
lipids
metabolism
description Neutral lipids - involved in many cellular processes - are stored as lipid droplets (LD), those mainly cytosolic (cLD) along with a small nuclear population (nLD). nLD could be involved in nuclear-lipid homeostasis serving as an endonuclear buffering system that would provide or incorporate lipids and proteins involved in signalling pathways as transcription factors and as enzymes of lipid metabolism and nuclear processes. Our aim was to determine if nLD constituted a dynamic domain. Oleic-acid (OA) added to rat hepatocytes or HepG2 cells in culture produced cellular-phenotypic LD modifications: increases in TAG, CE, C, and PL content and in cLD and nLD numbers and sizes. LD increments were reversed on exclusion of OA and were prevented by inhibition of acyl-CoA synthetase (with Triacsin C) and thus lipid biosynthesis. Under all conditions, nLD corresponded to a small population (2-10%) of total cellular LD. The anabolism triggered by OA, involving morphologic and size changes within the cLD and nLD populations, was reversed by a net balance of catabolism, upon eliminating OA. These catabolic processes included lipolysis and the mobilization of hydrolyzed FA from the LD to cytosolic-oxidation sites. These results would imply that nLD are actively involved in nuclear processes that include lipids. In conclusion, nLD are a dynamic nuclear domain since they are modified by OA through a reversible mechanism in combination with cLD; this process involves acyl-CoA-synthetase activity; ongoing TAG, CE, and PL biosynthesis. Thus, liver nLD and cLD are both dynamic cellular organelles.
format Articulo
Articulo
author Lagrutta, Lucía Carolina
Montero Villegas, Sandra
Layerenza, Juan Pablo
Sisti, Martín Sebastián
García de Bravo, Margarita María
Ves Losada, Ana
author_facet Lagrutta, Lucía Carolina
Montero Villegas, Sandra
Layerenza, Juan Pablo
Sisti, Martín Sebastián
García de Bravo, Margarita María
Ves Losada, Ana
author_sort Lagrutta, Lucía Carolina
title Reversible nuclear-lipid-droplet morphology induced by oleic acid: A link to cellular-lipid metabolism
title_short Reversible nuclear-lipid-droplet morphology induced by oleic acid: A link to cellular-lipid metabolism
title_full Reversible nuclear-lipid-droplet morphology induced by oleic acid: A link to cellular-lipid metabolism
title_fullStr Reversible nuclear-lipid-droplet morphology induced by oleic acid: A link to cellular-lipid metabolism
title_full_unstemmed Reversible nuclear-lipid-droplet morphology induced by oleic acid: A link to cellular-lipid metabolism
title_sort reversible nuclear-lipid-droplet morphology induced by oleic acid: a link to cellular-lipid metabolism
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87571
work_keys_str_mv AT lagruttaluciacarolina reversiblenuclearlipiddropletmorphologyinducedbyoleicacidalinktocellularlipidmetabolism
AT monterovillegassandra reversiblenuclearlipiddropletmorphologyinducedbyoleicacidalinktocellularlipidmetabolism
AT layerenzajuanpablo reversiblenuclearlipiddropletmorphologyinducedbyoleicacidalinktocellularlipidmetabolism
AT sistimartinsebastian reversiblenuclearlipiddropletmorphologyinducedbyoleicacidalinktocellularlipidmetabolism
AT garciadebravomargaritamaria reversiblenuclearlipiddropletmorphologyinducedbyoleicacidalinktocellularlipidmetabolism
AT veslosadaana reversiblenuclearlipiddropletmorphologyinducedbyoleicacidalinktocellularlipidmetabolism
bdutipo_str Repositorios
_version_ 1764820490211295233

Ejemplares similares