Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes

Bordetella parapertussis is a human pathogen that causes whooping cough. The increasing incidence of B. parapertussis has been attributed to the lack of cross protection induced by pertussis vaccines. It was previously shown that B. parapertussis is able to avoid bacterial killing by polymorphonucle...

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Autores principales: Gorgojo, Juan Pablo, Harvill, Eric T., Rodríguez, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Biología
Bordetella parapertussis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85560
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85560
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bordetella parapertussis
spellingShingle Biología
Bordetella parapertussis
Gorgojo, Juan Pablo
Harvill, Eric T.
Rodríguez, María Eugenia
Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes
topic_facet Biología
Bordetella parapertussis
description Bordetella parapertussis is a human pathogen that causes whooping cough. The increasing incidence of B. parapertussis has been attributed to the lack of cross protection induced by pertussis vaccines. It was previously shown that B. parapertussis is able to avoid bacterial killing by polymorphonuclear leukocytes (PMN) if specific opsonic antibodies are not present at the site of interaction. Here, we evaluated the outcome of B. parapertussis innate interaction with human macrophages, a less aggressive type of cell and a known reservoir of many persistent pathogens. The results showed that in the absence of opsonins, O antigen allows B. parapertussis to inhibit phagolysosomal fusion and to remain alive inside macrophages. The O antigen targets B. parapertussis to lipid rafts that are retained in the membrane of phagosomes that do not undergo lysosomal maturation. Fortyeight hours after infection, wild-type B. parapertussis bacteria but not the O antigen-deficient mutants were found colocalizing with lipid rafts and alive in nonacidic compartments. Taken together, our data suggest that in the absence of opsonic antibodies, B. parapertussis survives inside macrophages by preventing phagolysosomal maturation in a lipid raft- and O antigen-dependent manner. Two days after infection, about 15% of macrophages were found loaded with live bacteria inside flotillin-enriched phagosomes that had access to nutrients provided by the host cell recycling pathway, suggesting the development of an intracellular infection. IgG opsonization drastically changed this interaction, inducing efficient bacterial killing. These results highlight the need for B. parapertussis opsonic antibodies to induce bacterial clearance and prevent the eventual establishment of cellular reservoirs of this pathogen.
format Articulo
Articulo
author Gorgojo, Juan Pablo
Harvill, Eric T.
Rodríguez, María Eugenia
author_facet Gorgojo, Juan Pablo
Harvill, Eric T.
Rodríguez, María Eugenia
author_sort Gorgojo, Juan Pablo
title Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes
title_short Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes
title_full Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes
title_fullStr Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes
title_full_unstemmed Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes
title_sort bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85560
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AT rodriguezmariaeugenia bordetellaparapertussissurvivesinsidehumanmacrophagesinlipidraftenrichedphagosomes
bdutipo_str Repositorios
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