First molecular characterization of <i>Babesia vogeli</i> in two naturally infected dogs of Buenos Aires, Argentina

Large piroplasms (&gt;2.5 mm) were detected by direct microscopical investigation in 34 out of 16,767 (0.20%) canine blood smears in the Southern region of Greater Buenos Aires. Genomic DNA was extracted from two parasitemic dogs and the hypervariable 18S RNA gene region of the pathogen was spec...

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Autores principales: Eiras, Diego Fernando, Basabe, Julia, Mesplet, María, Schnittger, Leonhard
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2008
Materias:
Ciencias Veterinarias
Babesia vogeli
Canine babesiosis
PCR
Argentina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/150905
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-150905
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Babesia vogeli
Canine babesiosis
PCR
Argentina
spellingShingle Ciencias Veterinarias
Babesia vogeli
Canine babesiosis
PCR
Argentina
Eiras, Diego Fernando
Basabe, Julia
Mesplet, María
Schnittger, Leonhard
First molecular characterization of <i>Babesia vogeli</i> in two naturally infected dogs of Buenos Aires, Argentina
topic_facet Ciencias Veterinarias
Babesia vogeli
Canine babesiosis
PCR
Argentina
description Large piroplasms (&gt;2.5 mm) were detected by direct microscopical investigation in 34 out of 16,767 (0.20%) canine blood smears in the Southern region of Greater Buenos Aires. Genomic DNA was extracted from two parasitemic dogs and the hypervariable 18S RNA gene region of the pathogen was specifically amplified, sequenced, and aligned with corresponding gene sequences available in the GenBank. Phylogenetic trees were constructed and compared. 18S RNA gene sequences reliably segregated in three clearly distinguishable clades representing Babesia canis, Babesia vogeli and Babesia rossi isolates, respectively. The 18S RNA gene sequences of both Babesia isolates from Argentina affiliated to the B. vogeli branch. This finding represents the first molecular evidence of the existence of B. vogeli in Argentina.
format Articulo
Comunicacion
author Eiras, Diego Fernando
Basabe, Julia
Mesplet, María
Schnittger, Leonhard
author_facet Eiras, Diego Fernando
Basabe, Julia
Mesplet, María
Schnittger, Leonhard
author_sort Eiras, Diego Fernando
title First molecular characterization of <i>Babesia vogeli</i> in two naturally infected dogs of Buenos Aires, Argentina
title_short First molecular characterization of <i>Babesia vogeli</i> in two naturally infected dogs of Buenos Aires, Argentina
title_full First molecular characterization of <i>Babesia vogeli</i> in two naturally infected dogs of Buenos Aires, Argentina
title_fullStr First molecular characterization of <i>Babesia vogeli</i> in two naturally infected dogs of Buenos Aires, Argentina
title_full_unstemmed First molecular characterization of <i>Babesia vogeli</i> in two naturally infected dogs of Buenos Aires, Argentina
title_sort first molecular characterization of <i>babesia vogeli</i> in two naturally infected dogs of buenos aires, argentina
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/150905
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AT mespletmaria firstmolecularcharacterizationofibabesiavogeliiintwonaturallyinfecteddogsofbuenosairesargentina
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