Detection and identification of a soy protein component that cross-reacts with caseins from cow's milk
Soy-based formulas are the most employed cow's milk substitutes in the treatment of cow's milk allergy in our country. Since adverse reactions have been reported in allergic patients as a consequence of exposure to soy proteins, we have investigated the possible cross-reactivity between co...
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| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2002
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/127135 |
| Aporte de: |
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I19-R120-10915-127135 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas cross-reactivity soybean caseins ELISA Immunoblotting |
| spellingShingle |
Ciencias Exactas cross-reactivity soybean caseins ELISA Immunoblotting Rozenfeld, Paula Adriana Docena, Guillermo Horacio Añón, María Cristina Fossati, Carlos Alberto Detection and identification of a soy protein component that cross-reacts with caseins from cow's milk |
| topic_facet |
Ciencias Exactas cross-reactivity soybean caseins ELISA Immunoblotting |
| description |
Soy-based formulas are the most employed cow's milk substitutes in the treatment of cow's milk allergy in our country. Since adverse reactions have been reported in allergic patients as a consequence of exposure to soy proteins, we have investigated the possible cross-reactivity between components from soybean and cow's milk. A cow's milk specific polyclonal antiserum and casein specific monoclonal antibodies were used in immunoblotting and competitive ELISA studies to identify a 30-kD component from soybean that cross-reacts with cow's milk caseins. Its IgE binding capacity was tested by EAST, employing sera from cow's milk allergic patients, not previously exposed to soy proteins. The 30 kD protein was isolated and partially sequenced. It is constituted by two polypeptides (A₅ and B₃) linked by a disulphide bond. The protein's capacity to bind to the different antibodies relies on the B₃ poly-peptide. These results indicate that soy-based formula, which contains the A5-B3 glycinin molecule, could be involved in allergic reactions observed in cow's milk allergic patients exposed to soy-containing foods. |
| format |
Articulo Articulo |
| author |
Rozenfeld, Paula Adriana Docena, Guillermo Horacio Añón, María Cristina Fossati, Carlos Alberto |
| author_facet |
Rozenfeld, Paula Adriana Docena, Guillermo Horacio Añón, María Cristina Fossati, Carlos Alberto |
| author_sort |
Rozenfeld, Paula Adriana |
| title |
Detection and identification of a soy protein component that cross-reacts with caseins from cow's milk |
| title_short |
Detection and identification of a soy protein component that cross-reacts with caseins from cow's milk |
| title_full |
Detection and identification of a soy protein component that cross-reacts with caseins from cow's milk |
| title_fullStr |
Detection and identification of a soy protein component that cross-reacts with caseins from cow's milk |
| title_full_unstemmed |
Detection and identification of a soy protein component that cross-reacts with caseins from cow's milk |
| title_sort |
detection and identification of a soy protein component that cross-reacts with caseins from cow's milk |
| publishDate |
2002 |
| url |
http://sedici.unlp.edu.ar/handle/10915/127135 |
| work_keys_str_mv |
AT rozenfeldpaulaadriana detectionandidentificationofasoyproteincomponentthatcrossreactswithcaseinsfromcowsmilk AT docenaguillermohoracio detectionandidentificationofasoyproteincomponentthatcrossreactswithcaseinsfromcowsmilk AT anonmariacristina detectionandidentificationofasoyproteincomponentthatcrossreactswithcaseinsfromcowsmilk AT fossaticarlosalberto detectionandidentificationofasoyproteincomponentthatcrossreactswithcaseinsfromcowsmilk |
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Repositorios |
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