Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors

Background: Chagas disease is a parasitic infection caused by Trypanosoma cruzi. It is an important public health problem affecting around seven to eight million people in the Americas. A large number of hematophagous triatomine insect species, occupying diverse natural and human-modified ecological...

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Autores principales: Martínez Barnetche, Jesús, Lavore, Andrés E., Beliera, Melina, Téllez Sosa, Juan, Zumaya Estrada, Federico A., Palacio, Victorio, Godoy Lozano, Ernestina, Rivera Pomar, Rolando Víctor, Rodríguez, Mario Henry
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Exactas
Chagas disease
Reduviid bugs
Transcriptome
metabolism
oxidative phosphorylation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/108010
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5921304&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-108010
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Chagas disease
Reduviid bugs
Transcriptome
metabolism
oxidative phosphorylation
spellingShingle Ciencias Exactas
Chagas disease
Reduviid bugs
Transcriptome
metabolism
oxidative phosphorylation
Martínez Barnetche, Jesús
Lavore, Andrés E.
Beliera, Melina
Téllez Sosa, Juan
Zumaya Estrada, Federico A.
Palacio, Victorio
Godoy Lozano, Ernestina
Rivera Pomar, Rolando Víctor
Rodríguez, Mario Henry
Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors
topic_facet Ciencias Exactas
Chagas disease
Reduviid bugs
Transcriptome
metabolism
oxidative phosphorylation
description Background: Chagas disease is a parasitic infection caused by Trypanosoma cruzi. It is an important public health problem affecting around seven to eight million people in the Americas. A large number of hematophagous triatomine insect species, occupying diverse natural and human-modified ecological niches transmit this disease. Triatomines are long-living hemipterans that have evolved to explode different habitats to associate with their vertebrate hosts. Understanding the molecular basis of the extreme physiological conditions including starvation tolerance and longevity could provide insights for developing novel control strategies. We describe the normalized cDNA, full body transcriptome analysis of three main vectors in North, Central and South America, Triatoma pallidipennis, T. dimidiata and T. infestans. Results: Two-thirds of the de novo assembled transcriptomes map to the Rhodnius prolixus genome and proteome. A Triatoma expansion of the calycin family and two types of protease inhibitors, pacifastins and cystatins were identified. A high number of transcriptionally active class I transposable elements was documented in T. infestans, compared with T. dimidiata and T. pallidipennis. Sequence identity in Triatoma-R. prolixus 1:1 orthologs revealed high sequence divergence in four enzymes participating in gluconeogenesis, glycogen synthesis and the pentose phosphate pathway, indicating high evolutionary rates of these genes. Also, molecular evidence suggesting positive selection was found for several genes of the oxidative phosphorylation I, III and V complexes. Conclusions: Protease inhibitors and calycin-coding gene expansions provide insights into rapidly evolving processes of protease regulation and haematophagy. Higher evolutionary rates in enzymes that exert metabolic flux control towards anabolism and evidence for positive selection in oxidative phosphorylation complexes might represent genetic adaptations, possibly related to prolonged starvation, oxidative stress tolerance, longevity, and hematophagy and flight reduction. Overall, this work generated novel hypothesis related to biological adaptations to extreme physiological conditions and diverse ecological niches that sustain Chagas disease transmission.
format Articulo
Articulo
author Martínez Barnetche, Jesús
Lavore, Andrés E.
Beliera, Melina
Téllez Sosa, Juan
Zumaya Estrada, Federico A.
Palacio, Victorio
Godoy Lozano, Ernestina
Rivera Pomar, Rolando Víctor
Rodríguez, Mario Henry
author_facet Martínez Barnetche, Jesús
Lavore, Andrés E.
Beliera, Melina
Téllez Sosa, Juan
Zumaya Estrada, Federico A.
Palacio, Victorio
Godoy Lozano, Ernestina
Rivera Pomar, Rolando Víctor
Rodríguez, Mario Henry
author_sort Martínez Barnetche, Jesús
title Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors
title_short Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors
title_full Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors
title_fullStr Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors
title_full_unstemmed Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors
title_sort adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three chagas disease triatomine vectors
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/108010
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5921304&blobtype=pdf
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