Specific methionine oxidation of cytochrome c in complexes with zwitterionic lipids by hydrogen peroxide: potential implications for apoptosis
Cytochrome c (Cyt-c) has been previously shown to participate in cardiolipin (CL) oxidation and, therefore,in mitochondrial membrane permeabilization during the early events of apoptosis. The gain in this function has been ascribed to specific CL/Cyt-c interactions. Here we report that the cationic...
Guardado en:
Autores principales: | , , , , , , |
---|---|
Formato: | Articulo |
Lenguaje: | Inglés |
Publicado: |
2014
|
Materias: | |
Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/104719 http://hdl.handle.net/11336/5476 |
Aporte de: |
id |
I19-R120-10915-104719 |
---|---|
record_format |
dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Química Cytochrome c Apoptosis Hydrogen peroxide |
spellingShingle |
Química Cytochrome c Apoptosis Hydrogen peroxide Capdevila, Daiana Andrea Marmisollé, Waldemar Alejandro Tomasina, Florencia Demicheli, Verónica Portela, Magdalena Radi, Rafael Murgida, Daniel Horacio Specific methionine oxidation of cytochrome c in complexes with zwitterionic lipids by hydrogen peroxide: potential implications for apoptosis |
topic_facet |
Química Cytochrome c Apoptosis Hydrogen peroxide |
description |
Cytochrome c (Cyt-c) has been previously shown to participate in cardiolipin (CL) oxidation and, therefore,in mitochondrial membrane permeabilization during the early events of apoptosis. The gain in this function has been ascribed to specific CL/Cyt-c interactions. Here we report that the cationic protein Cyt-c is also able to interact electrostatically with the main lipid components of the mitochondrial membranes, the zwitterionic lipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE), through the mediation of phosphate anions that bind specifically to amino groups in the surfaces of protein and model membranes. In these complexes, Cyt-c reacts efficiently with H2O2 at submillimolar levels, which oxidizes the sulfur atom of the axial ligand Met80. The modified protein is stable and presents significantly enhanced peroxidatic activity. Based on these results, we postulate that the rise of H2O2 concentrations to the submillimolar levels registered during initiation of the apoptotic program may represent one signaling event that triggers the gain in peroxidatic function of the Cyt-c molecules bound to the abundant PE and PC membrane components. As the activated protein is a chemically stable species, it can potentially bind and oxidize important targets, such as CL. |
format |
Articulo Articulo |
author |
Capdevila, Daiana Andrea Marmisollé, Waldemar Alejandro Tomasina, Florencia Demicheli, Verónica Portela, Magdalena Radi, Rafael Murgida, Daniel Horacio |
author_facet |
Capdevila, Daiana Andrea Marmisollé, Waldemar Alejandro Tomasina, Florencia Demicheli, Verónica Portela, Magdalena Radi, Rafael Murgida, Daniel Horacio |
author_sort |
Capdevila, Daiana Andrea |
title |
Specific methionine oxidation of cytochrome c in complexes with zwitterionic lipids by hydrogen peroxide: potential implications for apoptosis |
title_short |
Specific methionine oxidation of cytochrome c in complexes with zwitterionic lipids by hydrogen peroxide: potential implications for apoptosis |
title_full |
Specific methionine oxidation of cytochrome c in complexes with zwitterionic lipids by hydrogen peroxide: potential implications for apoptosis |
title_fullStr |
Specific methionine oxidation of cytochrome c in complexes with zwitterionic lipids by hydrogen peroxide: potential implications for apoptosis |
title_full_unstemmed |
Specific methionine oxidation of cytochrome c in complexes with zwitterionic lipids by hydrogen peroxide: potential implications for apoptosis |
title_sort |
specific methionine oxidation of cytochrome c in complexes with zwitterionic lipids by hydrogen peroxide: potential implications for apoptosis |
publishDate |
2014 |
url |
http://sedici.unlp.edu.ar/handle/10915/104719 http://hdl.handle.net/11336/5476 |
work_keys_str_mv |
AT capdeviladaianaandrea specificmethionineoxidationofcytochromecincomplexeswithzwitterioniclipidsbyhydrogenperoxidepotentialimplicationsforapoptosis AT marmisollewaldemaralejandro specificmethionineoxidationofcytochromecincomplexeswithzwitterioniclipidsbyhydrogenperoxidepotentialimplicationsforapoptosis AT tomasinaflorencia specificmethionineoxidationofcytochromecincomplexeswithzwitterioniclipidsbyhydrogenperoxidepotentialimplicationsforapoptosis AT demicheliveronica specificmethionineoxidationofcytochromecincomplexeswithzwitterioniclipidsbyhydrogenperoxidepotentialimplicationsforapoptosis AT portelamagdalena specificmethionineoxidationofcytochromecincomplexeswithzwitterioniclipidsbyhydrogenperoxidepotentialimplicationsforapoptosis AT radirafael specificmethionineoxidationofcytochromecincomplexeswithzwitterioniclipidsbyhydrogenperoxidepotentialimplicationsforapoptosis AT murgidadanielhoracio specificmethionineoxidationofcytochromecincomplexeswithzwitterioniclipidsbyhydrogenperoxidepotentialimplicationsforapoptosis |
bdutipo_str |
Repositorios |
_version_ |
1764820442230554624 |