The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow's Milk Proteins

Reactions to soy have been reported in a proportion of patients with IgE-mediated cow’s milk allergy (CMA). In this work, we analyzed if Gly m Bd 28K/P28, one of the major soybean allergens, is a cross-reactive allergen with cow milk proteins (CMP). We showed that P28 was recognized by IgE sera from...

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Autores principales: Candreva, Ángela María, Smaldini, Paola Lorena, Curciarello, Renata, Fossati, Carlos Alberto, Docena, Guillermo Horacio, Petruccelli, Silvana
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Médicas
Food allergy
P28 soybean protein
Bovine caseins
Cross-reactivity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/101012
https://ri.conicet.gov.ar/11336/48714
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-101012
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Food allergy
P28 soybean protein
Bovine caseins
Cross-reactivity
spellingShingle Ciencias Médicas
Food allergy
P28 soybean protein
Bovine caseins
Cross-reactivity
Candreva, Ángela María
Smaldini, Paola Lorena
Curciarello, Renata
Fossati, Carlos Alberto
Docena, Guillermo Horacio
Petruccelli, Silvana
The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow's Milk Proteins
topic_facet Ciencias Médicas
Food allergy
P28 soybean protein
Bovine caseins
Cross-reactivity
description Reactions to soy have been reported in a proportion of patients with IgE-mediated cow’s milk allergy (CMA). In this work, we analyzed if Gly m Bd 28K/P28, one of the major soybean allergens, is a cross-reactive allergen with cow milk proteins (CMP). We showed that P28 was recognized by IgE sera from CMA patients and activated human peripheral basophils degranulation. Moreover, IgE sera of mice exclusively sensitized to CMP recognized P28. Splenocytes from sensitized animals secreted IL-5 and IL-13 when incubated with CMP or soy proteins, but only IL-13 when treated with P28. In addition, a skin test was strongly positive for CMP and weakly positive for P28. Remarkably, milk-sensitized mice showed hypersensitivity symptoms following sublingual challenge with P28 or CMP. With the use of bioinformatics’ tools seven putative cross-reactive epitopes were identified. In conclusion, using in vitro and in vivo tests we demonstrated that P28 is a novel cross-reactive allergen with CMP.
format Articulo
Preprint
author Candreva, Ángela María
Smaldini, Paola Lorena
Curciarello, Renata
Fossati, Carlos Alberto
Docena, Guillermo Horacio
Petruccelli, Silvana
author_facet Candreva, Ángela María
Smaldini, Paola Lorena
Curciarello, Renata
Fossati, Carlos Alberto
Docena, Guillermo Horacio
Petruccelli, Silvana
author_sort Candreva, Ángela María
title The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow's Milk Proteins
title_short The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow's Milk Proteins
title_full The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow's Milk Proteins
title_fullStr The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow's Milk Proteins
title_full_unstemmed The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow's Milk Proteins
title_sort major soybean allergen gly m bd 28k induces hypersensitivity reactions in mice sensitized to cow's milk proteins
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/101012
https://ri.conicet.gov.ar/11336/48714
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