Wastewater based epidemiology: population circulation dynamics of SARS-CoV-2 and its variants of concern (VOC) in Córdoba city and Punilla‘s Valley.
Abstract: Recent studies have shown that enteric cells are targets for SARS-CoV-2 infection, as they express the cellular receptor for the virus (ACE2), allowing viral replication in the gastrointestinal tract. Other studies report that 35% of patients infected with SARS-CoV-2 (sympto...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Artículo revista |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
|
| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/34900 |
| Aporte de: |
| Sumario: | Abstract:
Recent studies have shown that enteric cells are targets for SARS-CoV-2 infection, as they express the cellular receptor for the virus (ACE2), allowing viral replication in the gastrointestinal tract. Other studies report that 35% of patients infected with SARS-CoV-2 (symptomatic and asymptomatic) excrete the virus in fecal matter, with a mean excretion time of 17 days. In this way, wastewater constitutes a matrix that summarizes the population excretion of the virus. Objective: To study the population circulation dynamics of SARS-CoV-2 and its variants of concern (VOC) in wastewater from Córdoba city and Punilla‘s Valley. Materials and Methods: Sampling: From May 2020 to the present, 313 raw wastewater samples were collected from the central network conduit that enters the treatment plant of the city of Córdoba (n = 130) and Carlos Paz (n = 61), Valle Hermoso (n = 61) and La Falda (n = 61) treatment plants, in Punilla‘s Valley. Collection frequency: weekly. SARS-CoV-2 concentration in wastewater: elusion and precipitation with polyethylene glycol 6000, followed by 4°C differential centrifugations. RNA extraction: MagNAPure 96 - Roche. Genomic detection: DisCoVery SARS-CoV-2RT-PCR Detection Kit gen N and LightMix® Modular SARS and Wuhan CoV E-gene kit. Real-time RT-PCR Variants detection: TaqMan™ SARS-CoV-2 Mutation Panel (AppliedBiosystems) for detection of Alpha, Beta and Gamma VOCs. Results and Conclusions: Viral RNA was detected in 38% of the analyzed samples (28% Córdoba, 45% Punilla‘s Valley). SARS-CoV-2 genome detection, through Ct values, was correlated with the epidemiological curve of clinical cases reported between the months analyzed, been detected in water samples up to two weeks before the clinical cases exponential increase both in the first and second waves and in the summer outbreak in all localities. This predictive value of epidemic waves could be explained by a significant number of asymptomatic and pre-symptomatic excretors. In addition, wastewater study described the circulation dynamics of viral variants (VOC) in all the studied localities.
|
|---|