A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to elec...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Kluwer Academic Publishers
1987
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 04275caa a22005657a 4500 | ||
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| 003 | AR-BaUEN | ||
| 005 | 20230518203013.0 | ||
| 008 | 190411s1987 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-34250101643 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BTECE | ||
| 100 | 1 | |a Schijman, A.G. | |
| 245 | 1 | 2 | |a A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. |
| 260 | |b Kluwer Academic Publishers |c 1987 | ||
| 270 | 1 | 0 | |m Schijman, A.G.; Departamento de Química Biológica. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Benassi, F.O., Bloos, R.K., de Rambaldo, L.A., (1983) Rev. Arg. de Microbiol., 15, pp. 65-71 | ||
| 504 | |a Birnboim, H.C., Doly, J., (1979) Nucleic. Acids. Res., 7, pp. 1513-1523 | ||
| 504 | |a Blaschek, H.P., Klacik, M.A., (1984) J.Bacteriol., 147, pp. 262-266 | ||
| 504 | |a Davis, R.W., Botstein, D., Roth, J.R., (1980) advanced Bacterial Genetics Laboratory Manual, p. 201. , Cold Spring Harbor Laboratory, New York | ||
| 504 | |a Eckhardt, T., (1978) Plasmid, 1, pp. 584-588 | ||
| 504 | |a Floccari, M.E., Schijman, A.G., Méndez, B.S., (1986) Abstracts of the IV Southern Cone Meeting of the Panamerican Association of Biochemical Societies Nov26–29, , Pucon, Chile | ||
| 504 | |a Guerry, P., Le Blanc, D.J., Falkow, S., (1973) J. Bacteriol., 116, pp. 1064-1066 | ||
| 504 | |a Herrero, A.H., Gómez, R.F., (1980) Appl. Environ Microbiol., 40, pp. 571-577 | ||
| 504 | |a Jones, D.T., Jones, W.A., Woods, D.R., (1985) J.Gen. Microibol., 131, pp. 1213-1216 | ||
| 504 | |a Lin, Y.L., Blaschek, H.P., (1984) Appl. Environ. Microbiol., 48, pp. 737-742 | ||
| 504 | |a Maniatis, T., Fritsch, E.F., Sambrook, J., (1982) Molecular cloning, p. 160. , Cold Spring Harbor Laboratory, Cold Spring Harbor. New York | ||
| 504 | |a Meyers, J.A., Sánchez, D., Elwell, L.B., Falkow, S., (1976) J. Bacteriol., 127, pp. 1529-1537 | ||
| 504 | |a O'Brien, R.W., Morris, J.A., (1971) J. Gen. Microbiol., 68, pp. 307-318 | ||
| 504 | |a Oultram, J., Young, M., (1985) FEMS. Microbiol. Lett., 27, pp. 129-134 | ||
| 504 | |a Reysset, G., Sebald, M., (1985) Ann. Inst. Pasteur Microbiol., 136, pp. 275-282 | ||
| 504 | |a Schnaitman, C.A., (1971) J. Bacteriol., 108, pp. 553-563 | ||
| 504 | |a Strom, M.S., Eklund, Poysky, F., (1984) Appl.Env.Microbiol., 48, pp. 956-963 | ||
| 504 | |a Truffaut, N., Sebald, M., (1983) Mol.Gen. Genet., 189, pp. 178-180 | ||
| 504 | |a Urano, N., Karube, I., Suzoki, S., Yamada, T., (1983) Eur. J. Appl. Microbiol. Biotechnol., 17, pp. 349-354 | ||
| 504 | |a Youngman, P.J., Perkins, B.J., Losik, R., (1983) Proc. Natl. Acad.Sci. USA., 80, pp. 2305-2309 | ||
| 504 | |a Yu, P., Pearce, L.E., (1986) Biotechnol. Lett., 8, pp. 469-474 | ||
| 520 | 3 | |a A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to electrophoretic analysis. © 1987 Chapman & Hall. |l eng | |
| 593 | |a Departamento de Química Biológica. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina | ||
| 700 | 1 | |a Floccari, M.E. | |
| 700 | 1 | |a Méndez, B.S. | |
| 773 | 0 | |d Kluwer Academic Publishers, 1987 |g v. 1 |h pp. 163-168 |k n. 3 |p Biotechnol Tech |x 0951208X |w (AR-BaUEN)CENRE-1441 |t Biotechnology Techniques | |
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| 856 | 4 | 0 | |u https://doi.org/10.1007/BF00227554 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_0951208X_v1_n3_p163_Schijman |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951208X_v1_n3_p163_Schijman |y Registro en la Biblioteca Digital |
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| 999 | |c 61830 | ||