Determination of the profiles of secondary metabolites characteristic of Alternaria strains isolated from tomato
Background: Many Alternaria species have been studied for their ability to produce bioactive secondary metabolites, such as tentoxin (TEN), some of which have toxic properties. The main food contaminant toxins are tenuazonic acid, alternariol (AOH), alternariol monomethyl ether (AME), altenuene, and...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Español |
| Publicado: |
Asociacion Espanola de Micologia
2014
|
| Materias: | |
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | Background: Many Alternaria species have been studied for their ability to produce bioactive secondary metabolites, such as tentoxin (TEN), some of which have toxic properties. The main food contaminant toxins are tenuazonic acid, alternariol (AOH), alternariol monomethyl ether (AME), altenuene, and altertoxins i, ii and iii. Aims: To determine the profiles of secondary metabolites characteristic of Alternaria strains isolated from tomato for their chemotaxonomic classification. Methods: The profiles of secondary metabolites were determined by HPLC MS. Results: The Alternaria isolates obtained from spoiled tomatoes belong, according to their morphological characteristics, to the species groups Alternaria alternata, Alternaria tenuissima and Alternaria arborescens, with A. tenuissima being the most frequent. The most frequent profiles of secondary metabolites belonging to the species groups A. alternata (AOH, AME, TEN), A. tenuissima (AOH, AME, TEN, tenuazonic acid) and A. arborescens (AOH, AME, TEN, tenuazonic acid) were determined, with some isolates of the latter being able to synthesize AAL toxins. Conclusions: Secondary metabolite profiles are a useful tool for the differentiation of small spored Alternaria isolates not easily identifiable by their morphological characteristics. © 2013 Revista Iberoamericana de Micología. |
|---|---|
| Bibliografía: | Andersen, B., Frisvad, J., Natural occurrence of fungi and fungal metabolites in moldy tomatoes (2004) J Agric Food Chem, 52, pp. 7507-7513 Andersen, B., Hansen, M.E., Smedsgaard, J., Automated and unbiased image analyses as tools in phenotypic classification of small-spored Alternaria spp (2005) Phytopathology, 95, pp. 1021-1029 Andersen, B., Krøger, E., Roberts, R.G., Chemical and morphological segregation of Alternaria arborescens, A. infectoria and A. tenuissima species-groups (2002) Mycol Res, 106, pp. 170-182 Andrew, M., Peever, T.L., Pryor, B.M., An expanded multilocus phylogeny does not resolve morphological species within the small-spored Alternaria species complex (2009) Mycologia, 101, pp. 95-109 Azevedo, S.S., Mariano, R.L., Michereff, S.J., Survey of the intensity of black rot and Alternaria leaf spot of cabbage in Agreste of Pernambuco and determination of sample size for disease quantification (2000) Summa Phytopathol, 26, pp. 299-306 Bock, C.H., Thrall, P.H., Brubaker, C.L., Burdon, J.J., Detection of genetic variation in Alternaria brassicicola using AFLP fingerprinting (2002) Mycol Res, 106, pp. 428-434 Bottalico, A., Logrieco, A., (1992), pp. 209-232. , Alternaria plant diseases in Mediterranean countries and associated mycotoxin. En: Chelkowski J, Visconti A, editors. Alternaria, biology, plant disease and metabolites. Amsterdam: Elsevier;; Bottalico, A., Logrieco, A., (1998), pp. 65-108. , Toxigenic Alternaria species of economic importance. En: Sinha KK, Bhatnagar D, editors. Mycotoxins in agriculture and food safety. New York: Marcel Dekker Inc;; Cooke, D.E., Forster, J.W., Jenkins, P.D., Jones, D.G., Lewis, D.M., Analysis of intraspecific and interspecific variation in the genus Alternaria by the use of RAPD-PCR (1998) Ann Appl Biol, 132, pp. 197-209 Ellis, M.B., (1971), Dematiaceous hyphomycetes. Kew: Commomwealth Mycological Institute;; Filtenborg, O., Frisvad, J.C., Samson, R.A., (2002), pp. 306-320. , Specific association of fungi to foods and influence of physical environmental factors. En: Samson RA, Hoekstra ES, Frisvad JC, Filtenborg O, editors. Introduction to Food and airborne fungi. Baarn: Centraalbureau voor Schimmelcultures;; Frisvad, J.C., Andersen, B., Thrane, U., The use of secondary metabolite profiling in chemotaxonomy of filamentous fungi (2008) Mycol Res, 112, pp. 231-240 Kusaba, M., Tsuge, T., Nuclear ribosomal DNA variation and pathogenic specialization in Alternaria fungi known to produce host-specific toxins (1994) Appl Environ Microbiol, 60, pp. 3055-3062 Kusaba, M., Tsuge, T., Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA (1995) Curr Genet, 28, pp. 491-498 Kusaba, M., Tsuge, T., Mitochondrial DNA variation in host-specific toxin-producing pathogens in the genus Alternaria (1997) Ann Phytopathol Soc Jpn, 63, pp. 463-469 Logrieco, A., Bottalico, A., Mule, G., Moretti, A., Perrone, G., (2003), pp. 654-667. , Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. En: Xu X, Bailey JA, Cooke BM, editors. Epidemiology of mycotoxin producing fungi. Dordrecht: Kluwer Academic Publishers;; Logrieco, A., Moretti, A., Solfrizzo, M., Alternaria toxins and plant diseases: An overview of origin, occurrence and risks (2009) World Mycotoxin J, 2, pp. 129-140 Mislivec, P.B., Bruce, V.R., Stack, M.E., Bandler, R., Molds and tenuazonic acid in fresh tomatoes used for catsup production (1987) J Food Prot, 50, pp. 38-41 Morris, P.F., Connolly, M.S., St Clair, D.A., Genetic diversity of Alternaria alternata isolated from tomato in California assessed using RAPDs (2000) Mycol Res, 104, pp. 286-292 Nelson, P.E., Toussoun, T.A., Marasas, W.F., (1983) Fusarium species. An illustrated manual for identification, p. 12. , Pennsylvania State University Press, University Park, PA Nielsen, K.F., Smedsgaard, J., Larsen, T.O., Lund, F., Thrane, U., Frisvad, J.C., (2004), pp. 19-35. , Chemical identification of fungi: Metabolite profiling and metabolomics. En: Arora DK, editor. Fungal biotechnology in agricultural, food and environmental applications. New York: Marcel Dekker Inc;; Nishimura, S., Sugihara, M., Kohmoto, K., Otani, H., (1978), 13, pp. 1-10. , Two different phases in pathogenicity of the Alternaria pathogen causing black spot disease of Japanese pear. J Fac Agric Tottori Univ; Ostry, V., Alternaria mycotoxins: An overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs (2008) World Mycotoxin J, 1, pp. 175-188 Peever, T.L., Su, G., Carpenter-Boggs, L., Timmer, L.W., Molecular systematics of citrus-associated Alternaria species (2004) Mycologia, 96, pp. 119-134 Pitt, J.I., Hocking, A.D., (1977) Fungi and food spoilage, pp. 469-488. , Blackie Academic & Professional, London Polizzotto, R., Andersen, B., Martini, M., Grisan, S., Assante, G., Musetti, R., A polyphasic approach for the characterization of endophytic Alternaria strains isolated from grapevines (2012) J Microbiol Methods, 88, pp. 162-171 Pose, G., Patriarca, A., Kyanko, V., Pardo, A., Fernández Pinto, V., Effect of water activity and temperature on growth of Alternaria alternata on a synthetic tomato medium (2009) Int J Food Microbiol, 135, pp. 60-63 Pryor, B., Gilbertson, R., Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences (2000) Mycol Res, 104, pp. 1312-1321 Roberts, R.G., Reymond, S.T., Andersen, B., RAPD fragment pattern analysis and morphological segregation of small-spored Alternaria species and species groups (2000) Mycol Res, 104, pp. 151-160 Rotem, J., (1994) The genus Alternaria: Biology, epidemiology and pathogenicity, , The American Phytopathological Society, St Paul, MN Schulz, B., Boyle, C., Draeger, S., Römmert, A., Krohn, K., Endophytic fungi: A source of novel biologically active secondary metabolites (2002) Mycol Res, 106, pp. 996-1004 Simmons, E.G., (2007) Alternaria. An identification manual, , Centraalbureau voor Schimmelcultures, Utrecht Simmons, E.G., Roberts, R.G., Alternaria themes and variations (1993) Mycotaxon, 73, p. 109 Somma, S., Pose, G., Pardo, P., Mulé, G., Fernández Pinto, V., Moretti, A., AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree (2011) Int J Food Microbiol, 145, pp. 414-419 Terminiello, L., Patriarca, A., Pose, G., Fernández Pinto, V., Occurrence of alternariol, alternariol monomethyl ether and tenuazonic acid in Argentinean tomato puree (2006) Mycotoxin Res, 22, pp. 236-240 Weir, T.L., Huff, D.R., Christ, B.J., Romaine, C.P., RAPD-PCR analysis of genetic variation among isolates of Alternaria solani and A. alternata from potato and tomato (1998) Mycologia, 90, pp. 813-821 |
| ISSN: | 11301406 |
| DOI: | 10.1016/j.riam.2013.09.002 |