Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi
Gene expression in trypanosomes is controlled mainly by post-transcriptional processes. This study was designed to analyse HX1, one of the TcP2β upstream intergenic regions. It is an efficient pre-mRNA processing region that has been widely and successfully used in Trypanosoma cruzi transfection vec...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2005
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 09161caa a22009257a 4500 | ||
|---|---|---|---|
| 001 | PAPER-22201 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205344.0 | ||
| 008 | 190411s2005 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-13444267356 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MBIPD | ||
| 100 | 1 | |a Ben-Dov, C.P. | |
| 245 | 1 | 0 | |a Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi |
| 260 | |b Elsevier |c 2005 | ||
| 270 | 1 | 0 | |m Vázquez, M.P.; Lab. Biol. Molec. Enfermedad Chagas, INGEBI-CONICET, University of Buenos Aires, Vuelta de Obligado 2490 2P, 1428 Buenos Aires, Argentina; email: mvazquez@dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Vanhamme, L., Pays, E., Control of gene expression in trypanosomes (1995) Microbiol Rev, 59, pp. 223-240 | ||
| 504 | |a Liang, X.H., Haritan, A., Uliel, S., Michaeli, S., Trans and cis splicing in trypanosomatids: Mechanism, factors, and regulation (2003) Eukaryot Cell, 2, pp. 830-840 | ||
| 504 | |a Clayton, C.E., Life without transcriptional control? from fly to man and back again (2002) EMBO J, 21, pp. 1881-1888 | ||
| 504 | |a Sutton, R.E., Boothroyd, J.C., Evidence for trans-splicing in trypanosomes (1986) Cell, 47, pp. 527-535 | ||
| 504 | |a Lebowitz, J.H., Smith, H.Q., Rusche, L., Beverley, M., Coupling of poly (A) site selection and trans-splicing in Leishmania (1993) Genes Dev, 7, pp. 996-1007 | ||
| 504 | |a Matthews, K.R., Tschudi, C., Ullu, E., A common pyrimidine-rich motif governs trans-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes (1994) Genes Dev, 8, pp. 491-501 | ||
| 504 | |a Vasella, E., Braun, E., Roditi, I., Control of polyadenylation and alternative splicing of transcripts from adjacent genes in procyclic expression site: A dual role for polypirimidine tracts in trypanosomes? (1994) Nucleic Acids Res, 22, pp. 1359-1364 | ||
| 504 | |a Hartmann, C., Hotz, H.R., McAndrew, M., Clayton, C., Effect of multiple downstream splice sites on polyadenylation in Trypanosoma brucei (1998) Mol Biochem Parasitol, 93, pp. 149-152 | ||
| 504 | |a Patzelt, E., Perry, K., Agabian, N., Mapping of branch sites in trans-spliced pre-mRNAs of Trypanosoma brucei (1989) Mol Cell Biol, 9, pp. 4291-4297 | ||
| 504 | |a Hummel, H.S., Gilleespie, D.R., Swindle, J., Mutational analyses of 3′ splice site selection during trans-splicing (2000) J Biol Chem, 275, pp. 35522-35531 | ||
| 504 | |a Ullu, E., Tschudi, C., Günzl, A., Trans-splicing in trypanosomatid protozoa (1996) Frontiers in Molecular Biology: Molecular Biology of Parasitic Protozoa, pp. 115-133. , D.F. Smith M. Parsons Oxford | ||
| 504 | |a Vázquez, M.P., Levin, M.J., Functional analysis of the intergenic regions of TcP2β gene loci allowed the construction of an improved Trypanosoma cruzi vector (1999) Gene, 239, pp. 217-225 | ||
| 504 | |a Darocha, W.D., Otsu, K., Teixeira, S.M., Donelson, J.E., Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi (2004) Mol Biochem Parasitol, 133, pp. 175-186 | ||
| 504 | |a Lorenzi, H.A., Vazquez, M.P., Levin, M.J., Integration of expression vectors into the ribosomal locus of Trypanosoma cruzi (2003) Gene, 310, pp. 91-99 | ||
| 504 | |a Vázquez, M., Schijman, A., Levin, M.J., A short interspersed repetitive element provides a new 3′ acceptor site for trans-splicing in certain ribosomal TcP2β protein genes of Trypanosoma cruzi (1994) Mol Biochem Parasitol, 67, pp. 327-336 | ||
| 504 | |a Vázquez, M., Ben-Dov, C., Lorenzi, H., Moore, T., Schijman, A., Levin, M.J., The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons (2000) Proc Natl Acad Sci USA, 97, pp. 2128-2133 | ||
| 504 | |a Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K., Pease, L.R., Site directed mutagenesis by overlap extension using polymerase chain reaction (1989) Gene, 77, pp. 51-59 | ||
| 504 | |a Drozdz, M., Clayton, C., Structure of a regulatory 3′ untranslated region from Trypanosoma brucei (1999) RNA, 5, pp. 1632-1644 | ||
| 504 | |a Burge, C.B., Tuschl, T.H., Sharp, P.A., Splicing of precursors to mRNA by the spliceosomes (1999) The RNA World, pp. 525-560. , R.F. Gesteland T.R. Cech J.F. Atkins 2nd ed. Cold Spring Harbor Laboratory Press | ||
| 504 | |a Vázquez, M., Atorrasagasti, C., Bercovich, N., Volcovich, R., Levin, M.J., Unique features of the Trypanosoma cruzi U2AF35 splicing factor (2003) Mol Biochem Parasitol, 128 (1), pp. 77-81 | ||
| 504 | |a López-Estranio, C., Tschudi, C., Ullu, E., Exonic sequences in the 5′ untranslated region of α-tubulin mRNA modulate trans-splicing in Trypanosoma brucei (1998) Mol Cell Biol, 8, pp. 4620-4628 | ||
| 520 | 3 | |a Gene expression in trypanosomes is controlled mainly by post-transcriptional processes. This study was designed to analyse HX1, one of the TcP2β upstream intergenic regions. It is an efficient pre-mRNA processing region that has been widely and successfully used in Trypanosoma cruzi transfection vectors. Herein we compared its performance with other regions within the same locus, and we identified the sequence elements responsible for the HX1 efficiency in trans-splicing and protein synthesis. Our mutational analysis showed the flexibility of the branch point site selection for HX1 trans-splicing process. We demonstrated also that its 12 nt 5′UTR sequence contributes to both trans-splicing and translation efficiency. The natural insertion of the repetitive element short interspersed repetitive element (SIRE) in one of the HX1 polypyrimidine tracts decreases the translated protein level by 40%. In this report, we demonstrated that this reduction is a consequence of a decrease of five-fold in the level of processed mRNA balanced by an increased efficiency of translation due to the inclusion of a 38 nt SIRE specific sequence in the 5′UTR of the mRNA. © 2005 Elsevier B.V. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: TWAS, 00-311 RG/BIO/LA, PICT 01-06803 | ||
| 536 | |a Detalles de la financiación: Howard Hughes Medical Institute | ||
| 536 | |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica, PICT 01-05225 | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: We thank Dr. Sergio Angel, Dr. Esteban Serra and Dr. Juan Valcárcel for helpful comments to this manuscript. This work was supported by grants from FONCYT – PICT 01-05225 and TWAS research grant 00-311 RG/BIO/LA to MV, and FONCYT – PICT 01-06803, University of Buenos Aires, and World Health Organization/Special Program for Research and Training in Tropical Diseases (WHO/TDR) to MJL. In addition, MJL was supported in part by an International Research Scholar grant from the Howard Hughes Medical Institute, Chevy Chase, MD, USA. CBD had a fellowship from the University of Buenos Aires; MV and MJL are members of the career of scientific investigator of CONICET, Argentina. Appendix A | ||
| 593 | |a Lab. Biol. Molec. Enfermedad Chagas, INGEBI-CONICET, University of Buenos Aires, Vuelta de Obligado 2490 2P, 1428 Buenos Aires, Argentina | ||
| 593 | |a Programme of Gene Regulation, Centre for Genomic Regulation, 08003 Barcelona, Spain | ||
| 690 | 1 | 0 | |a CHAGAS DISEASE |
| 690 | 1 | 0 | |a POST-TRANSCRIPTIONAL REGULATION |
| 690 | 1 | 0 | |a REPETITIVE SEQUENCES |
| 690 | 1 | 0 | |a TRANS-SPLICING |
| 690 | 1 | 0 | |a MESSENGER RNA |
| 690 | 1 | 0 | |a RIBOSOME PROTEIN |
| 690 | 1 | 0 | |a 5' UNTRANSLATED REGION |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a GENE LOCUS |
| 690 | 1 | 0 | |a MUTATIONAL ANALYSIS |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN ANALYSIS |
| 690 | 1 | 0 | |a PROTEIN SYNTHESIS |
| 690 | 1 | 0 | |a RNA PROCESSING |
| 690 | 1 | 0 | |a TRANS SPLICING |
| 690 | 1 | 0 | |a TRANSCRIPTION REGULATION |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a INSERTION SEQUENCES |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Levin, M.J. | |
| 700 | 1 | |a Vázquez, M.P. | |
| 773 | 0 | |d Elsevier, 2005 |g v. 140 |h pp. 97-105 |k n. 1 |p Mol. Biochem. Parasitol. |x 01666851 |w (AR-BaUEN)CENRE-6136 |t Molecular and Biochemical Parasitology | |
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