Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements
The salivarian trypanosome Trypanosoma brucei infects mammals and is transmitted by tsetse flies. The mammalian 'bloodstream form' trypanosome has a variant surface glycoprotein coat and relies on glycolysis while the procyclic form from tsetse flies has EP protein on the surface and has a...
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2002
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| LEADER | 17279caa a22017297a 4500 | ||
|---|---|---|---|
| 001 | PAPER-20282 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205141.0 | ||
| 008 | 190411s2002 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0037109118 | |
| 024 | 7 | |2 cas |a 3' Untranslated Regions; Adenine, 73-24-5; Antigens, Surface; ELAV-like protein 1; Phosphoglycerate Kinase, EC 2.7.2.3; Protozoan Proteins; RNA, Messenger; RNA, Protozoan; RNA-Binding Proteins; Uracil, 66-22-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a NARHA | ||
| 100 | 1 | |a Quijada, L. | |
| 245 | 1 | 0 | |a Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements |
| 260 | |c 2002 | ||
| 270 | 1 | 0 | |m Clayton, C.; ZMBH, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany; email: cclayton@zmbh.uni-heidelberg.de |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a The salivarian trypanosome Trypanosoma brucei infects mammals and is transmitted by tsetse flies. The mammalian 'bloodstream form' trypanosome has a variant surface glycoprotein coat and relies on glycolysis while the procyclic form from tsetse flies has EP protein on the surface and has a more developed mitochondrion. We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA in bloodstream forms. The human HuR protein binds to, and stabilises, mammalian mRNAs containing AREs. Expression of HuR in bloodstream-form trypanosomes resulted in growth arrest and in stabilisation of the EP, PGKB and pyruvate, phosphate dikinase mRNAs, while three bloodstream-specific mRNAs were reduced in abundance. The synthesis and abundance of unregulated mRNAs and proteins were unaffected. Our results suggest that regulation of mRNA stability by AREs arose early in eukaryotic evolution. |l eng | |
| 536 | |a Detalles de la financiación: EMBO | ||
| 536 | |a Detalles de la financiación: Deutsche Forschungsgemeinschaft | ||
| 536 | |a Detalles de la financiación: We thank Henry Furneaux (Memorial Sloan Kettering Cancer Center, New York, NY) for HuR and c-fos plasmids, and for the anti-HuR monoclonal antibody. We thank Steve Beverley (University of Washington, MO, USA) and Keith Matthews (Manchester University, UK) for very useful criticism and suggestions, Shula Michaeli (Bar-Ilan University, Israel) for the SRP RNA clone and Paul Michels (ICP, Brussels) for anti-PGK. We thank other members of the laboratory, particularly A. Estévez, for discussions, and L. Krauth-Siegel, BZH, Heidelberg, for the DHLADH antibody. We thank Gitta Erdmann for contributions made during an undergraduate laboratory rotation. L.Q. was supported by an EMBO fellowship, and C.G. by a DAAD scholarship. C.B.-D. contributed during a short-term visit funded by the DAAD. The rest of the support came from the Deutsche Forschungsgemeinschaft. | ||
| 593 | |a ZMBH, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany | ||
| 593 | |a Centro de Biología Molecular 'SO', Lab. CX-203, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain | ||
| 593 | |a Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland | ||
| 593 | |a Yale University School of Medicine, LLCI 8o1, 333 Cedar Street, New Haven, CT 06520-8022, United States | ||
| 593 | |a INGEBI, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ADENINE |
| 690 | 1 | 0 | |a MESSENGER RNA |
| 690 | 1 | 0 | |a PHOSPHOGLYCERATE KINASE |
| 690 | 1 | 0 | |a PROTOZOAL PROTEIN |
| 690 | 1 | 0 | |a PYRUVIC ACID |
| 690 | 1 | 0 | |a RNA BINDING PROTEIN |
| 690 | 1 | 0 | |a URACIL |
| 690 | 1 | 0 | |a VARIANT SURFACE GLYCOPROTEIN |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DISEASE TRANSMISSION |
| 690 | 1 | 0 | |a EUKARYOTE |
| 690 | 1 | 0 | |a EVOLUTION |
| 690 | 1 | 0 | |a GLYCOLYSIS |
| 690 | 1 | 0 | |a GROWTH INHIBITION |
| 690 | 1 | 0 | |a MITOCHONDRION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a PROTEIN RNA BINDING |
| 690 | 1 | 0 | |a PROTEIN STABILITY |
| 690 | 1 | 0 | |a RNA STABILITY |
| 690 | 1 | 0 | |a RNA STRUCTURE |
| 690 | 1 | 0 | |a RNA SYNTHESIS |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA BRUCEI |
| 690 | 1 | 0 | |a TSETSE FLY |
| 690 | 1 | 0 | |a 3' UNTRANSLATED REGIONS |
| 690 | 1 | 0 | |a ADENINE |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIGENS, SURFACE |
| 690 | 1 | 0 | |a BASE SEQUENCE |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION, DEVELOPMENTAL |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a MOLECULAR SEQUENCE DATA |
| 690 | 1 | 0 | |a PHOSPHOGLYCERATE KINASE |
| 690 | 1 | 0 | |a PROTOZOAN PROTEINS |
| 690 | 1 | 0 | |a REGULATORY SEQUENCES, NUCLEIC ACID |
| 690 | 1 | 0 | |a RNA STABILITY |
| 690 | 1 | 0 | |a RNA, MESSENGER |
| 690 | 1 | 0 | |a RNA, PROTOZOAN |
| 690 | 1 | 0 | |a RNA-BINDING PROTEINS |
| 690 | 1 | 0 | |a TRYPANOSOMA BRUCEI BRUCEI |
| 690 | 1 | 0 | |a URACIL |
| 690 | 1 | 0 | |a EUKARYOTA |
| 690 | 1 | 0 | |a GLOSSINA (PROPER) |
| 690 | 1 | 0 | |a MAMMALIA |
| 690 | 1 | 0 | |a PROTOZOA |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA BRUCEI |
| 700 | 1 | |a Guerra-Giraldez, C. | |
| 700 | 1 | |a Drozdz, M. | |
| 700 | 1 | |a Hartmann, C. | |
| 700 | 1 | |a Irmer, H. | |
| 700 | 1 | |a Ben-Dov, C. | |
| 700 | 1 | |a Cristodero, M. | |
| 700 | 1 | |a Ding, M. | |
| 700 | 1 | |a Clayton, C. | |
| 773 | 0 | |d 2002 |g v. 30 |h pp. 4414-4424 |k n. 20 |p Nucleic Acids Res. |x 03051048 |w (AR-BaUEN)CENRE-1769 |t Nucleic Acids Research | |
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