Insulin inhibits δ-aminolevulinate synthase gene expression in rat hepatocytes and human hepatoma cells
Insulin has been known to regulate intracellular metabolism by modifying the activity or location of many enzymes but it is only in the past few years that the regulation of gene expression is recognized to be a major action of this hormone. The present work provides evidences that insulin inhibits...
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Academic Press Inc.
1998
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| LEADER | 15427caa a22013217a 4500 | ||
|---|---|---|---|
| 001 | PAPER-19776 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205109.0 | ||
| 008 | 190411s1998 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0032212803 | |
| 024 | 7 | |2 cas |a 5 aminolevulinate synthase, 9037-14-3; insulin, 9004-10-8; protein kinase C, 141436-78-4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ECREA | ||
| 100 | 1 | |a Scassa, M.E. | |
| 245 | 1 | 0 | |a Insulin inhibits δ-aminolevulinate synthase gene expression in rat hepatocytes and human hepatoma cells |
| 260 | |b Academic Press Inc. |c 1998 | ||
| 270 | 1 | 0 | |m Canepa, E.T.; Departamento de Quimica Biologica, Facultad de Cie. Exactas/Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina; email: ecanepa@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Cheatham, B., Kahn, C.R., Insulin action and the insulin signaling network (1995) Endocrin. Rev., 16, pp. 117-142 | ||
| 504 | |a O'Brien, R.M., Granner, D.K., Regulation of gene expression by insulin (1996) Physiol. Rev., 76, pp. 1109-1161 | ||
| 504 | |a White, M.F., Kahn, C.R., The insulin signaling system (1994) J. Biol. Chem., 269, pp. 1-4 | ||
| 504 | |a Saltiel, A.R., The paradoxical regulation of protein phosphorylation in insulin action (1994) FASEB J., 8, pp. 1034-1040 | ||
| 504 | |a Myers, M.G., Jian Sun, X., White, M.F., The IRS-1 signaling system (1994) Trends Biochem. Sci., 19, pp. 289-293 | ||
| 504 | |a O'Brien, R.M., Granner, D.K., Regulation of gene expression by insulin (1991) Biochem. J., 278, pp. 609-619 | ||
| 504 | |a Meisler, M.H., Howard, G., Effects of insulin on gene transcription (1989) Annu. Rev. Physiol., 51, pp. 701-714 | ||
| 504 | |a O'Brien, R.M., Lucas, P.C., Forest, C.D., Magnuson, M.A., Granner, D.K., Identification of a sequence in the phosphoenolpyruvate carboxykinase gene that mediates a negative effect of insulin on transcription (1990) Science, 249, pp. 533-537 | ||
| 504 | |a Prager, D., Melmed, S., Insulin regulates expression of the human growth hormone gene in transfected cells (1988) J. Biol. Chem., 263, pp. 16580-16585 | ||
| 504 | |a Stanley, F.M., An element in the prolactin promoter mediates the stimulatory effect of insulin on transcription of the prolactin gene (1992) J. Biol. Chem., 267, pp. 16719-16726 | ||
| 504 | |a Shimura, Y., Shimura, H., Ohmori, M., Ikuyama, S., Kohn, L., Identification of a novel insulin-responsive element in the rat thyrotropin receptor promoter (1994) J. Biol. Chem., 269, pp. 31908-31914 | ||
| 504 | |a Jacob, K.K., Ouyang, L., Stanley, F.M., A consensus insulin response element is activated by an ETS-related transcription element (1995) J. Biol. Chem., 270, pp. 27773-27779 | ||
| 504 | |a Giger, U., Meyer, U.A., Induction of δ-aminolevulinate synthase and cytochrome P-450 hemoproteins in hepatocytes culture. Effect of glucose and hormones (1981) J. Biol. Chem., 256, pp. 11182-11190 | ||
| 504 | |a Cánepa, E.T., Llambías, E.B.C., Grinstein, M., Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from normal and experimental-diabetic rats. Role of Insulin (1990) Biochem. Cell Biol., 68, pp. 914-921 | ||
| 504 | |a Moore, M.R., Biochemistry of porphyria (1993) Int. J. Biochem., 25, pp. 1353-1368 | ||
| 504 | |a Srivastava, G., Hansen, A.J., Bawden, M.J., May, B.K., Hemin administration to rats reduces levels of hepatic mRNAs for phenobarbitone-inducible enzymes (1990) Mol. Pharmacol., 38, pp. 486-493 | ||
| 504 | |a Dematteis, F., Marks, G.S., Cytochrome P-450 and its interaction with the heme biosynthetic pathway (1996) Can. J. Physiol. Pharmacol., 74, pp. 1-8 | ||
| 504 | |a Dogra, S.C., Hahn, C.N., May, B.K., Superinduction by cycloheximide of cytochrome P4502H1 and 5-aminolevulinate synthase gene transcription in chick embryo liver (1993) Arch. Biochem. Biophys., 300, pp. 531-534 | ||
| 504 | |a Varone, C.L., Cánepa, E.T., Llambías, E.B.C., Grinstein, M., CAMP regulation of phenobarbital-mediated induction of δ-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats (1994) Biochem. Cell Biol., 72, pp. 381-390 | ||
| 504 | |a Varone, C.L., Cánepa, E.T., Evidence that protein kinase C is involved in δ-aminolevulinate synthase expression in rat hepatocytes (1997) Arch. Biochem. Biophys., 341, pp. 259-266 | ||
| 504 | |a Messina, J.L., Insulin's regulation of c-fos (1990) J. Biol. Chem., 265, pp. 11700-11705 | ||
| 504 | |a O'Brien, R.M., Bonovich, M.T., Forest, C.D., Granner, D.K., Signal transduction convergence: Phorbol esters and insulin inhibit phophoenolpyruvate carboxykinase gene transcription through the same 10-base-pair-sequence (1991) Proc. Natl. Acad. Sci. USA, 88, pp. 6580-6584 | ||
| 504 | |a Messina, J.L., Standaert, M.L., Ishizuka, T., Weinstock, R.S., Farese, R.V., Role of protein kinase C in insulin's regulation of c-fos (1992) J. Biol. Chem., 267, pp. 9223-9228 | ||
| 504 | |a Sutherland, C., O'Brien, R.M., Granner, D.K., Phosphatidylinositol 3-kinase, but not p70/p85 ribosomal S6 protein kinase, is required for the regulation of phosphoenolpyruvate carboxykinase (PEPCK) gene expression by insulin (1995) J. Biol. Chem., 270, pp. 15501-15506 | ||
| 504 | |a Fry, J.R., Jones, C.A., Webkin, P., Bridges, J.W., The enzymic isolation of adult rat hepatocytes in a functional and viable state (1976) Anal. Biochem., 71, pp. 341-350 | ||
| 504 | |a Cánepa, E.T., Llambías, E.B.C., Grinstein, M., Effect of glucose on induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide (1984) Biochem. Biophys. Acta, 804, pp. 8-15 | ||
| 504 | |a Chomczynski, P., Sacchi, N., Single-step method of RNA isolation by acid guanidium-thiocyanate-phenol-chloroform extraction (1987) Anal. Biochem., 162, pp. 156-159 | ||
| 504 | |a Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY | ||
| 504 | |a Varone, C.L., Cánepa, E.T., Llambías, E.B.C., Grinstein, M., Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes (1996) Biochem. Cell Biol., 74, pp. 271-281 | ||
| 504 | |a Yamamoto, M., Kure, S., Engel, J.D., Hiraga, K., Structure, turnover, and heme-mediated supression of the level of mRNA encoding rat liver δ-aminolevulinate synthase (1988) J. Biol. Chem., 263, pp. 15973-15979 | ||
| 504 | |a Boshart, M., Klüppel, M., Schmidt, A., Schütz, G., Luckow, B., Reporter constructs with low background activity utilizing thecat (1992) Gene, 110, pp. 129-130 | ||
| 504 | |a Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K., Introduction of DNA into mammalian cells (1992) Current Protocols in Molecular Biology, , Wiley | ||
| 504 | |a Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254 | ||
| 504 | |a Edlund, T., Walker, M.D., Barr, P.J., Rutter, W.J., Cell-specific expression of the rat insulin gene: Evidence for role of two distinct 5′ flanking elements (1985) Science, 230, pp. 912-916 | ||
| 504 | |a Seed, B., Sheen, J.Y., A single phase-extraction assay for chloramphenicol acetyltransferase activity (1988) Gene, 67, pp. 271-277 | ||
| 504 | |a Hargrove, J.L., Schmidt, F.H., The role of mRNA and protein stability in gene expression (1989) FASEB J., 3, pp. 2360-2370 | ||
| 504 | |a Hanson, R.W., Reshef, L., Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression (1997) Annu. Rev. Biochem., 66, pp. 581-611 | ||
| 504 | |a Kappas, A., Sassa, S., Galbraith, R.A., Nordmann, Y., The porphyrias (1989) The Metabolic Basis of Inherited Diseases, , New York: McGraw-Hill. p. 1305-1365 | ||
| 504 | |a Sasaki, K., Cripe, T.P., Koch, S.R., Andreone, T.L., Petersen, D.D., Beale, E.G., Granner, D.K., Multihormonal regulation of phosphoenolpyruvate carboxykinase gene transcription (1984) J. Biol. Chem., 259, pp. 15242-15251 | ||
| 504 | |a Ooi, G., Brown, D., Suh, D., Tseng, L., Rechler, M., Cycloheximide stabilizes insulin-like growth factor-binding protein-1 (IGFBP-1) mRNA and inhibits IGFBP-1 transcription in H4-II-E rat hepatoma cells (1993) J. Biol. Chem., 268, pp. 16664-16672 | ||
| 504 | |a Newton, R., Stevens, D., Hart, I., Lindsay, M., Adcock, I., Barnes, P., Superinduction of COX-2 mRNA by cycloheximide and interleukin-1beta involves increased transcription and correlates with increased NF-kappaB and JNK activation (1997) FEBS Lett., 418, pp. 135-138 | ||
| 504 | |a Zinck, R., Cahill, M., Kracht, M., Sachsenmaier, C., Hipskind, R., Nordheim, A., Protein synthesis inhibitors reveal differential regulation of mitogen-activated protein kinase and stress-activated protein kinase pathways that converge on Elk-1 (1995) Mol. Cell. Biol., 15, pp. 4930-4938 | ||
| 504 | |a Rolland, V., Dugah, Y., Le Liepvre, X., Lavau, M., Evidence of increased glyceraldehyde-3-phosphate dehydrogenase and fatty acid synthetase promoter activities in transiently transfected adipocytes from genetically obese rats (1995) J. Biol. Chem., 270, pp. 1102-1106 | ||
| 504 | |a Yamahuchi, K., Holt, K., Pessin, J.E., Phosphatidylinositol 3-kinase functions upstream of Ras and Raf in mediating insulin stimulation ofc-fos (1993) J. Biol. Chem., 268, pp. 14597-14600 | ||
| 504 | |a Jacob, K.K., Stanley, F.M., Insulin and cyclic adenosine monophosphate increase prolactin gene expression through different response pathways (1995) Mol. Cell Endocrinol., 109, pp. 175-181 | ||
| 504 | |a O'Brien, R.M., Noisin, E.L., Suwanichkul, A., Yamasaki, T., Lucas, P.C., Wang, J.C., Powell, D.R., Granner, D.K., Hepatic nuclear factor-3 and hormone-regulated espression of the phosphoenolpyruvate carboxykinase and insulin-like growth factor-binding protein 1 genes (1995) Mol. Cell. Biol., 15, pp. 1747-1758 | ||
| 504 | |a Roux, J., Pictet, R., Grange, T., Hepatocyte nuclear factor 3 determines the amplitude of the glucocorticoid response of the rat tyrosine aminotransferase gene (1995) DNA Cell Biol., 14, pp. 385-396 | ||
| 504 | |a Robertson, D.G., Marino, E.M., Thule, P.M., Seneviratne, C.K., Murphy, L.J., Insulin and glucocorticoids regulate IGFBP-1 expression via a common promoter region (1994) Biochem. Biophys. Res. Commun., 200, pp. 226-232 | ||
| 504 | |a Quinn, P.G., Inhibition by insulin of protein kinase A-induced transcription of the phosphoenolpyruvate carboxykinase gene (1994) J. Biol. Chem., 269, pp. 14375-14378 | ||
| 504 | |a Nakajima, T., Fukamizu, A., Takahashi, J., Gage, F.H., Fisher, T., Blenis, J., Montminy, M.R., The signal-dependent coactivator CBP is a nuclear target for pp90RSK (1996) Cell, 86, pp. 465-474 | ||
| 504 | |a Kolch, W., Heidecker, G., Kochs, G., Hummel, R., Vahidi, H., Mischak, H., Finkenzeller, G., Rapp, U.R., Protein kinase C alpha activates RAF-1 by direct phosphorylation (1993) Nature, 364, pp. 249-252 | ||
| 504 | |a Gabbay, R.A., Sutherland, C., Gnudi, L., Kahn, B.B., O'Brien, R.M., Granner, D.K., Flier, J.S., Insulin regulation of phosphoenolpyruvate carboxykinase gene expression does not require activation of the Ras/mitogen-activated protein kinase signaling pathway (1996) J. Biol. Chem., 271, pp. 1890-1897 | ||
| 504 | |a Messina, J., Weinstock, R., Evidence for diverse roles of protein kinase-C in the inhibition of gene expression by insulin: The tyrosine aminotransferase, albumin, and phosphoenolpyruvate carboxykinase genes (1994) Endocrinology, 135, pp. 2327-2334 | ||
| 520 | 3 | |a Insulin has been known to regulate intracellular metabolism by modifying the activity or location of many enzymes but it is only in the past few years that the regulation of gene expression is recognized to be a major action of this hormone. The present work provides evidences that insulin inhibits δ- aminolevulinate synthase (ALA-S) gene expression, the enzyme which governs the rate-limiting step in heme biosynthesis. The addition of 5 nM insulin to hepatocytes culture led to a significant decrease of both basal and phenobarbital-induced ALA-S mRNA in a dose-dependent manner, as measured by Northern and slot-blot analysis. Several clues as to how insulin regulates ALA-S transcription were determined. The inhibitory effect is achieved at physiological concentrations but much higher proinsulin doses are needed. Insulin's effect is rapid, quite specific, and protein synthesis is not required. Moreover, ALA-S mRNA half-life is not modified by the presence of the peptidic hormone. Our results demonstrate that the insulin effect is dominant; it overrides 8-CPT-cAMP plus phenobarbital-mediated induction. Also, insulin requires the activation of protein kinase C to exert its full effect. On the other hand, a 870-bp fragment of the ALA-S promoter region is able to sustain the inhibition of CAT expression in plasmid-transfected HepG2 cells. Thus, these results indicate that insulin plays an important role in regulating ALA-S expression by inhibiting its transcription. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: 1This work was supported by research grants from the Univer-sidad de Buenos Aires and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina). | ||
| 593 | |a Lab. de Biología Molecular, Regulacion de la Expresion Genica, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a GENE EXPRESSION |
| 690 | 1 | 0 | |a HEME |
| 690 | 1 | 0 | |a HEPATIC CELLS |
| 690 | 1 | 0 | |a INSULIN |
| 690 | 1 | 0 | |a PROTEIN KINASE |
| 690 | 1 | 0 | |a Δ-AMINOLEVULINATE SYNTHASE |
| 690 | 1 | 0 | |a 5 AMINOLEVULINATE SYNTHASE |
| 690 | 1 | 0 | |a INSULIN |
| 690 | 1 | 0 | |a MESSENGER RNA |
| 690 | 1 | 0 | |a PROTEIN KINASE C |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ENZYME ACTIVATION |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION |
| 690 | 1 | 0 | |a HEPATOMA CELL |
| 690 | 1 | 0 | |a HORMONE ACTION |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a LIVER CELL |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a NORTHERN BLOTTING |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 700 | 1 | |a Varone, C.L. | |
| 700 | 1 | |a Montero, L. | |
| 700 | 1 | |a Cánepa, E.T. | |
| 773 | 0 | |d Academic Press Inc., 1998 |g v. 244 |h pp. 460-469 |k n. 2 |p Exp. Cell Res. |x 00144827 |w (AR-BaUEN)CENRE-797 |t Experimental Cell Research | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00144827_v244_n2_p460_Scassa |y Handle |
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