Development of a novel methodology for cryopreservation of melanoma cells applied to CSF470 therapeutic vaccine
CSF470 vaccine is a mixture of four lethally irradiated melanoma cell lines, administered with BCG and GM-CSF, which is currently being tested in a Phase II/III Clinical trial in stage II/III melanoma patients. To prepare vaccine doses, irradiated melanoma cell lines are frozen using dimethyl sulfox...
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| Otros Autores: | , , , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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2013
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 13040caa a22013097a 4500 | ||
|---|---|---|---|
| 001 | PAPER-11230 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204121.0 | ||
| 008 | 190411s2013 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84883555383 | |
| 024 | 7 | |2 cas |a dimethyl sulfoxide, 67-68-5; human serum albumin, 9048-49-1; trehalose, 99-20-7 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a CRYBA | ||
| 100 | 1 | |a Tapia, I.J. | |
| 245 | 1 | 0 | |a Development of a novel methodology for cryopreservation of melanoma cells applied to CSF470 therapeutic vaccine |
| 260 | |c 2013 | ||
| 270 | 1 | 0 | |m Barrio, M.M.; Centro de Investigaciones Oncológicas, Fundación Cáncer FUCA, Cramer 1180, CP 1426, Buenos Aires, Argentina; email: mmbarrio@conicet.gov.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Schebor, C., Mazzobre, M.F., Buera, M.P., Glass transition and time-dependent crystallization behavior of dehydration bioprotectant sugars (2010) Carbohydr. Res., 345, pp. 303-308 | ||
| 504 | |a Tai, T., Cahan, L.D., Tsuchida, T., Saxton, R.E., Irie, R.F., Morton, D.L., Immunogenicity of melanoma-associated gangliosides in cancer patients (1985) Int. J. Cancer, (5), pp. 607-612 | ||
| 504 | |a von Euw, E.M., Barrio, M.M., Furman, D., Bianchini, M., Levy, E.M., Yee, C., Li, Y., Mordoh, J., Monocyte-derived dendritic cells loaded with a mixture of apoptotic/necrotic melanoma cells efficiently cross-present gp100 and MART-1 antigens to specific CD8(+) T lymphocytes (2007) J. Transl. Med., 20, p. 19 | ||
| 504 | |a von Euw, E.M., Barrio, M.M., Furman, D., Levy, E.M., Bianchini, M., Peguillet, I., Lantz, O., Mordoh, J., A phase I clinical study of vaccination of melanoma patients with dendritic cells loaded with allogeneic apoptotic/necrotic melanoma cells. Analysis of toxicity and immune response to the vaccine and of IL-10 -1082 promoter genotype as predictor of disease progression (2008) J. Transl. Med., 6, p. 6 | ||
| 504 | |a Wikström, J., Elomaa, M., Nevala, L., Räikkönen, J., Heljo, P., Urtti, A., Yliperttula, M., Viability of freeze dried microencapsulated human retinal pigment epithelial cells (2012) Eur. J. Pharm. Sci., 47, pp. 520-526 | ||
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| 504 | |a Wolkers, W.F., Loopera, S.A., Fontanilla, R.A., Tsvetkova, N.M., Tablin, F., Crowe, J.H., Temperature dependence of fluid phase endocytosis coincides with membrane properties of pig platelets (2003) Biochimica et Biophysica Acta, 1612, pp. 154-163 | ||
| 504 | |a Wolkers, W.F., Walker, N.J., Tablin, F., Crowe, J.H., Human platelets loaded with trehalose survive freeze-drying (2001) Cryobiology, 42, pp. 79-87 | ||
| 504 | |a Wright, D.L., Eroglu, A., Toner, M., Toth, T.L., Use of sugars in cryopreserving human oocytes (2004) Reprod. Biomed. Online, 9 (2), pp. 179-186 | ||
| 504 | |a https://register.clinicaltrials.gov/prs/app/action/SelectProtocol?sid=S0003VL3&selectaction=View&uid=U0001TJV&ts=8&cx=gvgb80, ClinicalTrials.gov: CASVAC0401 registered at | ||
| 520 | 3 | |a CSF470 vaccine is a mixture of four lethally irradiated melanoma cell lines, administered with BCG and GM-CSF, which is currently being tested in a Phase II/III Clinical trial in stage II/III melanoma patients. To prepare vaccine doses, irradiated melanoma cell lines are frozen using dimethyl sulfoxide (Me2SO) and stored in liquid nitrogen (liqN2). Prior to inoculation, doses must be thawed, washed to remove Me2SO and suspended for clinical administration. Avoiding the use of Me2SO and storage in liqN2 would allow future freeze-drying of CSF470 vaccine to facilitate pharmaceutical production and distribution. We worked on the development of an alternative cryopreservation methodology while keeping the vaccine's biological and immunogenic properties. We tested different freezing media containing trehalose suitable to remain as excipients in a freeze-dried product, to cryopreserve melanoma cells either before or after gamma irradiation. Melanoma cells incorporated trehalose after 5h incubation at 37°C by fluid-phase endocytosis, reaching an intracellular concentration that varied between 70-140mM depending on the cell line. Optimal freezing conditions were 0.2M trehalose and 30mg/ml human serum albumin, at -84°C. Vaccine doses could be frozen in trehalose at -84°C for at least four months keeping their cellular integrity, antigen expression and apoptosis/necrosis profile after gamma-irradiation as compared to Me2SO control. Non-irradiated melanoma cell lines also showed comparable proliferative capacity after both cryopreservation procedures. Trehalose-freezing medium allowed us to cryopreserve melanoma cells, either alive or after gamma irradiation, at -84°C avoiding the use of Me2SO and liqN2 storage. These cryopreservation conditions could be suitable for future freeze-drying of CSF470 vaccine. © 2013 Elsevier Inc. |l eng | |
| 593 | |a Centro de Investigaciones Oncológicas FUCA, Ciudad Autónoma de Buenos Aires, Crámer 1180, Primer Piso, CP1426, Argentina | ||
| 593 | |a Laboratorio de Propiedades Fisicoquímicas y Conservación de Biomoléculas, Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina | ||
| 593 | |a Laboratorio Pablo Cassará, Ciudad Autónoma de Buenos Aires, Caaguazú 7171, CP1408, Argentina | ||
| 593 | |a Instituto de Investigaciones Bioquímicas de Buenos Aires, CONICET and Fundación Instituto Leloir, Patricias Argentinas 435 (CP1405), Ciudad Autónoma de Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CRYOPRESERVATION |
| 690 | 1 | 0 | |a MELANOMA VACCINE |
| 690 | 1 | 0 | |a TREHALOSE |
| 690 | 1 | 0 | |a CSF 470 |
| 690 | 1 | 0 | |a DIMETHYL SULFOXIDE |
| 690 | 1 | 0 | |a HUMAN SERUM ALBUMIN |
| 690 | 1 | 0 | |a LIQUID NITROGEN |
| 690 | 1 | 0 | |a MELANOMA VACCINE |
| 690 | 1 | 0 | |a TREHALOSE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ANTIGEN EXPRESSION |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL DEATH |
| 690 | 1 | 0 | |a CELL LEVEL |
| 690 | 1 | 0 | |a CELL PROLIFERATION |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a CRYOPRESERVATION |
| 690 | 1 | 0 | |a ENDOCYTOSIS |
| 690 | 1 | 0 | |a FREEZING |
| 690 | 1 | 0 | |a GAMMA IRRADIATION |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a LOW TEMPERATURE |
| 690 | 1 | 0 | |a MELANOMA CELL |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROCESS OPTIMIZATION |
| 690 | 1 | 0 | |a THAWING |
| 690 | 1 | 0 | |a CRYOPRESERVATION |
| 690 | 1 | 0 | |a MELANOMA VACCINE |
| 690 | 1 | 0 | |a TREHALOSE |
| 690 | 1 | 0 | |a CANCER VACCINES |
| 690 | 1 | 0 | |a CELL LINE, TUMOR |
| 690 | 1 | 0 | |a CRYOPRESERVATION |
| 690 | 1 | 0 | |a CRYOPROTECTIVE AGENTS |
| 690 | 1 | 0 | |a DIMETHYL SULFOXIDE |
| 690 | 1 | 0 | |a FREEZING |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a MELANOMA |
| 690 | 1 | 0 | |a TREHALOSE |
| 653 | 0 | 0 | |a csf 470 |
| 700 | 1 | |a Aris, M. | |
| 700 | 1 | |a Arriaga, J.M. | |
| 700 | 1 | |a Blanco, P.A. | |
| 700 | 1 | |a Mazzobre, F. | |
| 700 | 1 | |a Vega, J. | |
| 700 | 1 | |a Mordoh, J. | |
| 700 | 1 | |a Barrio, M.M. | |
| 773 | 0 | |d 2013 |g v. 67 |h pp. 163-169 |k n. 2 |p Cryobiology |x 00112240 |w (AR-BaUEN)CENRE-4349 |t Cryobiology | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00112240_v67_n2_p163_Tapia |y Handle |
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